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20191
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Subject: Plant Pathology × Author: SHARMA, SHIVANI ×
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    ThesisItemOpen Access
    STUDIES ON PRODUCTION AND ANTIMICROBIAL PROPERTIES OF Volvariella volvacea (Bull.Ex.Fr.) Sing
    (COLLEGE OF HORTICULTURE AND FORESTRY, DR Y S P UHF, NERI, HAMIRPUR, 2019-10-23) SHARMA, SHIVANI; JARIAL, R S
    The present investigations entitled “Studies on production and antimicrobial properties of Volvariella volvacea (Bull.Ex.Fr.) Sing’’ was undertaken to evaluate various cultural requirements for the vegetative growth of different strains of V. volvacea, to evaluate different locally available substrates for the production of different strains of V. volvacea and to study antimicrobial properties of different strains of V. volvacea against five human pathogenic bacteria viz., E. Coli, Proteus sp., Bacillus sp., Pseudomonas sp. and Streptococcus sp. Among five liquid media studied, malt extract was found to be the best medium for the growth of all the strains of V. volvacea both in solid (38.92 mm) as well as liquid (2625.42 mg) phase. Out of five different pH levels evaluated for the growth of different strains of V. volvacea, pH 7.0 (30.75 mm) was observed to be the best pH for the growth of all the strains of the test fungus. Out of five different temperature regimes evaluated, 30°C (41.78 mm) was observed to be the ideal temperature for the growth of V. volvacea. Out of four strains under study DMR-484 was observed to grow best in all the nutrient media (Solid; 41.38 mm and Liquid; 1780.67 mg), different pH levels (29.64 mm) and different temperature regimes (37.00 mm) studied. In production trials, among five different substrates, cotton waste was observed to be the best substrate in terms of spawn run period (8.25 days), time taken for first flush (13.91 days), number of fruit bodies (35.50/bed), yield (472.91 g/Kg) and biological efficiency (15.76%). The aqueous and methanolic extracts of the mycelium of strain DMR-484 were found to have antimicrobial activities against E. coli with average zone of inhibition 10.33 mm and 13.00 mm, respectively. However, mycelia of all other strains were unable to inhibit any of five human pathogenic bacteria under study. Further, it was also found that none of the four fruit body extracts of all the strains under study exhibited antimicrobial activity against any of the human pathogenic bacteria.