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Subject: Microbiology × Author: BALKAR, SINGH × End date: 2016 × Start date: 2010 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    ISOLATION AND CHARACTERIZATION OF PLANT GROWTH PROMOTING RHIZOBACTERIA (PGPR) FROM TURMERIC (Curcuma longa L.)
    (2013) BALKAR, SINGH; KAUSHAL, RAJESH
    ABSTRACT Plant growth promoting rhizobacteria (PGPR) due to myriad plant growth promoting/stimulating traits have become an emerging area of research and important commercial agro-input in past two decades. They have acquired a strategic place in sustainable agriculture development around the globe. Supplementation of chemical fertilizers by PGPR can reduce chemical input upto 25-30%. Beside this PGPR also provides a low cost eco-friendly way of detoxification and bioremediation of soil. Hence, investigation entitled “Isolation and characterization of plant growth promoting rhizobacteria (PGPR) from Turmeric (Curcuma longa L.)” was carried out during the years 2010-12 for the development a potential bio-inoculum for turmeric crop growing in low mid hills of Himachal Pradesh. The rhizospheric soil samples, rhizome/root samples of Turmeric (Curcuma longa L.) plants were collected from Kangra (31º2 to 32º5 N and 75º to 77º45 E) and Hamirpur (31º25 to 31º52 N and 76º18 to 76º44 E) districts of Himachal Pradesh. Using modified replica plating technique a sum of 27 isolates were selected and purified. Only 7 isolates out of 27 isolates were selected for net house studies, on the basis of various PGPR traits (N-fixation, P-solubilization, sidrophore, HCN and auxin production) and antagonism against Pythium spp. and collectotrichum spp. causing rhizome rot and leaf spot respectively. Bacterial isolate A10 showed maximum P-solubilization qualitative (81.5%) and quantitative (128.8μg/ml) as well, growth on Jensen’s medium, sidrophpre production (103.7%), IAA production (29.4μg/ml), HCN production and antagonism against Pythium spp.(41.4%) and collectotrichum spp.(39.3%). Bacterial isolate A10 tentatively identified as Bacillus spp. on the basis of its morphological, physiological and biochemical characteristics. Plants bacterized with isolate A10 showed significantly increase in leaf number (33.1%), shoot length (16.5%), shoot biomass (28.4%), root length (29.2%), root biomass (52.3%), rhizome size (58.8%) and rhizome yield (54.6%) over uninoculated control, along with maximum NPK uptake and availability. Biofertization and bioprotactant characters of selected bacterial isolate A10 (Bacillus spp.) paved the way of its use at commercial level to supplement chemical fertilizers for enhance production after conducting multilocational field trial in different agro-climatic zones of the state.