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ThesisItem Open Access A STUDY ON SHEEP PRODUCTION PRACTICES IN GUNTUR DISTRICT OF ANDHRA PRADESH(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2011) SIREESHA, K; PRASAD, R.M.V(MAJOR)ABSTRACT : A study was undertaken to study various sheep production practices followed by the shepherds and to record the physical parameters of sheep in Guntur district. A total of 240 farmers selected at random from three divisions of the district were interviewed by a questionnaire schedule and required information was collected. Out of the total farmers studied, 92.5% were illiterates and 7.5% were literates. Among all the shepherds, 44.58% belonged to BC community, 20% to SC community, 15% to ST community and 20.42% to other communities. It was further recorded that out of the total farmers studied, 52.5 % were landless, 37.92 % were marginal, 7.08 % were small and 2.50 % were large farmers. It was observed that all the farmers in the area were rearing sheep extensively, 95% of the farmers studied were providing housing to their sheep and out of which 60.70% of the shepherds housed their sheep nearer to their houses. 91.70% of the farmers grazed their animals for duration of 8-10 h, while the grazing distance ranged from 2-4 km (67.90 %) to 4-6 km (32.10%). Flock mating commonly followed and flushing was not at all practiced by the shepherds. June – August and January – March were found to be the breeding seasons for the sheep in the area. From the study it was observed that the marketing of meat was done mainly through middlemen (87.08%). The average body length observed in different age groups of male sheep were 45.69 ± 0.66, 67.13 ± 0.90, 74.04 ± 0.39, 76.54 ± 0.54, 77.32 ± 0.49 and 78.23 ± 0.47 cm for 0-3 month, 4-12 month, 2 teeth, 4 teeth, 6 teeth and full mouth animals, respectively while the corresponding values for female sheep were 44.14 ± 0.80, 62.75 ± 0.60, 70.03 ± 0.37, 70.78 ± 0.41, 71.19 ± 0.36 and 70.29 ± 0.42 cm, respectively. The mean body height of male sheep in different age groups were 50.71 ± 0.79, 69.14 ± 0.85, 77.15 ± 0.43, 81.09 ± 0.54, 83.47 ± 0.43 and 82.80 ± 0.43 cm for 0-3 month, 4-12 month, 2 teeth, 4 teeth, 6 teeth and full mouth animals, respectively while the corresponding values for female sheep were 49.49 ± 1.13, 65.24 ± 0.59, 73.42 ± 0.31, 75.48 ± 0.38, 76.55 ± 0.44 and 77.97 ± 0.37 cm, respectively. It was further observed that the mean body girth measurements of male sheep in Guntur district were 52.01 ± 1.01, 70.86 ± 0.92, 81.60 ± 0.46, 85.64 ± 0.64, 89.97 ± 0.52 and 88.84 ± 0.60 cm for 0-3 month, 4-12 month, 2 teeth, 4 teeth, 6 teeth and full mouth animals, respectively while the corresponding values for females were 48.82 ± 1.05, 67.28 ± 0.62, 77.02 ± 0.31, 79.36 ± 0.36, 81.86 ± 0.38 and 83.46 ± 0.63 cm, respectively. The study revealed that the mean body weights of male sheep in Guntur district were 11.05 ± 0.55, 22.13 ± 0.77, 34.96 ± 0.50, 38.57 ± 0.50, 43.14 ± 0.33 and 44.56 ± 0.76 kg for 0-3 month, 4-12 month, 2 teeth, 4 teeth, 6 teeth and full mouth animals, respectively whiles the corresponding values for females were 9.60 ± 0.64, 19.92 ± 0.55, 27.36 ± 0.22, 30.20 ± 0.39, 32.91 ± 0.28 and 34.37 ± 0.36 kg, respectively.ThesisItem Open Access CLINICO-BIOCHEMICAL AND THERAPEUTIC STUDIES OF RUMINAL ACIDOSIS IN GOATS(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2013-12) TAMBE SATISH VALMIK; PADMAJA, K(MAJOR); NAGARAJ, P; GOPALA REDDY, AABSTRACT:The present study was designed to investigate the clinico-biochemical parameters and response to therapeutic agents in clinical cases of goats suffering with ruminal acidosis. Thirty clinical cases of ruminal acidosis presented to the Teaching Veterinary Clinical Complex, Campus Veterinary Hospital, and Veterinary Ambulatory Clinic, Mylardevpally, College of Veterinary Science, Rajendranagar, Hyderabad with the history of accidental ingestion of carbohydrate rich diet, inappetance to anorexia, suspended rumination and clinical manifestations of distended rumen and diarrhoea were selected and were divided based on severity of ruminal acidosis into three different groups consisting of 10 goats in each. The group I (mild acidosis), group II (moderate acidosis), and group III (severe acidosis) goats were subjected to the therapeutic trials and compared against ten apparently healthy goats from the same area with similar husbandry practices which served as healthy control (Group IV). Clinical signs exhibited by mild acidotic goats (Group I) were inappetance, mild bloat, reduced rumen motility, reduced rumination, semisolid faeces, firm and doughy condition of rumen on palpation of left flank and dull sound on percussion of left flank. The signs observed in moderate acidotic goats (Group II) were anorexia, distended abdomen, absence of rumination, dull appearance, absence of ruminal motility, grinding of teeth, frequent bleating, thick nasal discharge, pasty faeces, dehydration, fluid flashing sound on percussion and gurgling sound on auscultation of rumen. Whereas in severe acidotic goats (Group III), the signs were observed anorexia, severe depression, sunken eyes, staggering gait, lameness, absence of rumination, ruminal motility absent, watery diarrhoea, dehydration, oligouria to anuria, dysponea, tachycardia, subnormal body temperature, sternal and lateral recumbency. Clinical signs after treatment in all affected goats disappeared. Ruminal acidotic goats had subnormal body temperature, heart and respiration rates and skin fold time in all affected groups of goats significantly (p<0.05) increased, ruminal motility significantly (p<0.05) decreased in group I and absent in group II and III, conjunctival mucous membrane was normal, slightly congested and congested in group I, group II and III respectively. There was no significant difference in the values of temperature, heart and respiration rates, skin fold time and ruminal motility in all affected groups of goats and conjunctival mucous membrane was normal after treatment. Rumen fluid samples were analysed for physical changes. Colour was gray in group I and milky gray in both group II and III, consistency was semisolid or slightly watery in group I and watery in both group II and III, odour was acidic in both group I and II and pungent acidic in group I, and significant (p<0.05) decrease in pH between the groups of affected goats before therapy. Micro-biochemical changes of rumen fluid like MBRT was significantly (p<0.05) increased in all affected groups of goats, SAT was significantly (p<0.05) increased in group I and absent in group II and III, iodophilic activity was reduced in group I and nil in both group II and III, protozoal motility was sluggish in group I and absent in both group II and III, protozoal density decreased in group I and absent in both group II and III, significant (p<0.05) decrease in live and increase in dead proportion in group I, complete absence of protozoa in both group II and III, and replacement of rumen fluid gram negative bacteria by gram positive bacteria in all affected groups of goats before treatment. Rumen fluid analysis was normal in all the groups after the treatment. In haematological parameters, a significant (p<0.05) increase in haemoglobin, packed cell volume, total leucocyte count, neutrophils, eosinophils, monocytes and significant (p<0.05) decrease in lymphocytes in all affected groups of goats before treatment. Haematological parameters were retured to normal in all the groups after the treatment. Blood biochemical analysis revealed significant (p<0.05) increase in plasma glucose, serum blood urea nitrogen and creatinine, serum aspartate aminotransferase and serum lactate dehydrogenase enzymes were observed in all affected groups of goats before treatment. After treatment all blood biochemical parameters returned to normalcy in all the groups after the treatment. The mean values of urine pH before treatment was significantly (P < 0.05) decreased between the all affected groups as compared with group IV. The mean values of urine pH did not differ significantly after treatment in group I, II and III. The Benedict’s test of urine was positive in all affected goats before treatment as compared to group IV. After therapy, it was negative in all ruminal acidosis affected goats. The therapeutic efficacy of the drugs used against various types of ruminal acidosis in goats was assessed. Group I goats were given sodium bicarbonate @ 1 g/kg body weight, single dose orally daily for 5 days. Group II goats were given ‘Bufzone’ @ 50 g single dose orally daily for 4 days. Whereas, goats of group III were administered ‘Bufzone’ @ 50 g single dose orally daily for 4 days and Sodium bicarbonate @ 1 g/kg body weight orally for 5 days. In addition goats of all the three groups were administered with Chlorphenarmine maleate @ 0.5 mg/kg body weight im, Tribivet @ 2ml im and Ringer’s lactate @ 25 ml, 50-75 ml, 75-74 100 ml iv in group I, II and III respectively. It was concluded that ruminal acidosis is a common disease of goats and its severity can be effectively reduced by combination of Bufzone and sodium bicarbonate along with supportive therapy.ThesisItem Open Access CHOLESTEROL ASSIMILATION ABILITY OF Lactobacillus paraplantarum 321 and Bifidobacterium bifidum 231 IN MILK FAT RICH PRODUCTS BY IN VITRO EVALUATION(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2013-11) PARAM DEBBARMA; KONDAL REDDY, K(MAJOR); SHASHI KUMAR, M; MADHAVA RAO, T; SARAT CHANDRA, AABSTRACT: A strain of Lactobacillus paraplantarum321 and a strain of Bifidobacterium bifidum 231were studied for their cholesterol assimilation ability and growth in MRS broth with added ghee/water soluble cholesterol (polyoxyethanyl-cholesterylsebacate, Sigma, USA), and supplemented with 0.1 and 0.3% oxgall after 24 and 48 h incubation respectively. The residual concentration of cholesterol of added ghee in the pellet cells of MRS broth was also determined. Scanning electron microscopy was also performed to ascertain whether cholesterol binds to the cellular surface of probiotics. Results showed that a considerable variation existed between the two strains and at two levels of bile salts in their cholesterol assimilation ability and the mean probiotic bacterial counts (log 10 CFU/mL). In broth added with ghee, both the strains were able to assimilate cholesterol at varying levels ranging from to 13.24-26.16 μg/mL or 13.46-26.61% and ranging from 17.50- 30.38 μg/mL or 18.03-31.31% after 24 and 48 h incubation. Highest cholesterol assimilation ability was achieved by Lactobacillus paraplantarum321 26.61% (0.3% oxgall) and 31.31% (0.3% oxgall) after 24 and 48 h incubation respectively. In broth with added water soluble cholesterol, both strains also assimilated cholesterol at varying levels ranging from to 16.18-35.15 μg/mL or 17.05-37.00% and ranging from 24.35-40.02 μg/mL or 25.71-42.26% after 24 and 48 h incubation. Highest cholesterol assimilation ability was carried out by Lactobacillus paraplantarum321 37.00% (0.3% oxgall) and 42.26% (0.3% oxgall) after 24 and 48 h incubation respectively. Cholesterol removed by pellet cells of both strains ranged from 12.77-18.80 μg/mL or 12.99-19.12% and 15.42-22.84 μg/mL or 15.89-23.53% after 24 and 48 h incubation in MRS broth. The mean probiotic bacteria counts (log 10 CFU/mL) of Lactobacillus paraplantarum 321 and Bifidobacterium bifidum 231 in both added ghee/water soluble cholesterol after 24 and 48 h incubation, decreased significantly with an increasing concentration of oxgall when compared to the control. In broth with added ghee, Lactobacillus paraplantarum 321 at 0.3% oxgall concentration showed least bacterial counts 9.78±0.09 log 10 CFU/mL and 9.51±0.09 log 10 CFU/mL after 24 and 48 h incubation. While, in broth with added water soluble cholesterol, Bifidobacterium bifidum 231 at 0.3% oxgall concentration showed least bacterial count 9.55±0.04 log 10 CFU/mL after 24 h incubation and Lactobacillus paraplantarum 321showed least bacterial counts 9.44±0.12 log 10 CFU/mL after 48 h incubation. The scanning electron microscopy of the tested probiotics strains did not reveal any traces of cholesterol binding on the cellular surface of organisms as reported by some authors when the samples are processed with standard dehydration solvents. However, when the samples were processed without dehydration using alcohol, it did reveal some traces of cholesterol binding on cellular surface of organisms. From this study, it may be concluded that, the probiotic strains i.e. Lactobacillus paraplantarum 321 and Bifidobacterium bifidum 231 have the ability to assimilate cholesterol from the laboratory media. Lactobacillus paraplantarum 321 was found to assimilate more cholesterol than Bifidobacterium bifidum 231. The oxgall concentration of 0.3% resulted in higher reduction of cholesterol than 0.1% oxgall by both above strains. The cholesterol removal ability is higher initially upto 24 h and slows down upto 48 h thereafter. The SEM sample preparation method may influence the observation of cholesterol binding on the cellular surface of organism.ThesisItem Open Access STUDIES ON PESTICIDE RESIDUES IN WATER, SOIL, FODDER AND MILK ALONG MUST RIVER BELT(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2013-10) KOTINAGU, KORRAPATI; KRISHNAIAH, N(MAJOR); MADAHAVA RAO, T; KONDAL REDDY, K; SASHI BHUSHAN, VABSTRACT: A study was conducted to estimate residues of certain pesticides of organochlorines viz., DDT (o,p'- DDE, o,p' - DDD, p,p'- DDT and o,p'- DDT and Dicofol), HCH (Alpha, Beta, Gamma, Delta), cyclodiene compounds (Aldrin, Endosulfan Sulphate and Heptachlor) and organophosphates (Triazophos, Dimetheoate, Chlorpyrifos and Methyl-chlorpyrifos) in soil, water, fodder and milk samples collected from six zones of Musi river belt area. To evaluate the pollution level of Musi river, the river belt was divided in to six zones viz., Zone 1 (Attapur to High court), Zone 2 (Chadhar ghat to Uppal), Zone 3 (Peerzadiguda to Chinna viralla), Zone 4 (Pillai Palli to Alinagar), Zone 5 (Indriyala to Manimadde), Zone 6 (Musi reservoir to Wazirabad). Only soil samples collected hm Zone 1 showed residual levels (in ppm) of 0.06 + 0.005 (0.035 to 0.083), 0.73 * 0.01 (0.675 to 0.791), 1.27 * 0.09 (1.023 to l.893), 0.14 =k 0.015 (0.098 to 0.243) and 0.55 * 0.02 (0.481 to 0.685) for p,p'- DDE, o,p'- DDD, p,p'- DDT, o,p' - DDT and Total DDT respectively. Dicofol was present only in fodder samples of zone 5 at concentration of 0.07 + 0.0007 (0.071 to 0.077). Among HCH compounds only delta HCH was found in soil samples of Zone 1 at a concentration of 0.08 *0.003 (0.065 to 0.098). Water, fodder and milk samples from zone 2 to 6 did not contain any residues of DDT and HCH. None of the samples water, soil, fodder and Milk from all the 6 zones contain the residues of Cyclodiene compounds. Among organophosphorus compounds Triazophos was present in soil samples of zone 1 at a level of 0.03 * 0.001 (0.032 to 0.045) and Dimetheoate was present in milk samples collected from Zone 6 at a level of 0.13 & 0.006 (0.1 11 to 0.167). From this study, it can be concluded that all the pesticide residues in soil were well below the MRL values, whereas Dicofol in fodder and Dimethoate in milk were slightly above the MRL values specified by EU and CODEX.ThesisItem Open Access ASSESSMENT OF GENETIC DIVERSITY IN ONGOLE AND PUNGANUR CATTLE BREEDS THROUGH MICROSATELLITE TYPING(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2013-04) ASHA, UPPUTURI; Sakunthala Devi, K(MAJOR); Rajkumar, U; Harikrishna, ChABSTRACT: Twenty dinucleotide microsatellite markers viz ETHOLO, ILSTSOOS, TGLA122, INRA035, INRA063, HEL001, TGLA 126, INRAOOS, ILSTS006, INRA032, ETH225, CSRM060, BMI 824, TGLA053, INRAO37, ETHO03, TGLA227, MM012, HAUT024 and HAUT027 were used for Assessment of genetic diversity in Ongole and Punganur cattle breeds through Microsatellite typing. The mean quantity of DNA was 2.96 pdml in Ongole and 3.16 pg/ml in Punganur cattle. Mean optical absorbance ratio (2601280nm) was 1.78 in both Ongole and Punganur cattle indicating good quality of genomic DNA. Out of the total 216 alleles detected, 98 alleles were specific to Ongole and 75 alleles were specific to Punganur. Mean number of alleles obtained at each locus varied from 1 to 12 in Ongole and 1 to 13 in Punganur cattle. The overall mean effective number of alleles was found to be 4.09 in Ongole and 3.41 in Punganur cattle. The overall mean expected heterozygosity and observed heterozygosity were 0.65 and 0.33 in Ongole and 0.55 and 0.3 1 in Punganur breed, respectively. The overall mean PIC values observed were 0.62 and 0.53 in Ongole and Punganur breeds respectively. The mean inbreeding coefficient (Fls) obtained in the present study was 0.456 for Ongole and 0.434 in Punganur breed. F-statistics revealed moderate inbreeding within the breeds and existence of moderate relationship between the Ongole and Punganur cattle. In Ongole population, loci ETHO10, TGLA122, INRA035, INRA063, HEL001, ILSTS006, TGLA126, ETH225, CSRM60, TGLA053, INRA037, ETH003, MM012, HAUT027 and HAUT024 were deviated significantly from the equilibrium frequency (I'c0.01). In Punganur breed, except the locus TGLA126, ETH225 and CSRMO60, all other loci deviated significantly (Ps0.01) from the Hardy-Weinberg equilibrium.ThesisItem Open Access EFFECT OF FEEDING MAIZE STOVER BASED COMPLETE RATIONS WITH DIFFERENT ROUGHAGE TO CONCENTRATE RATIO ON PERFORMANCE IN NELLORE X DECCANI RAM LAMBS(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2013-02) VENKATESHWARLU, V; VENKATESHWARLU, M(MAJOR); RAMANA REDDY, Y; HARI KRISHA, ChABSTRACT : An experiment was conducted for 120 days to investigate the effect of feeding maize (Zea mays) stover based complete rations (mash form) at MS70 (maize stover 70% + concentrate 30%), MS60 (maize stover 60% + concentrate 40%) and MS50 (maize stover 50% + concentrate 50%) ratios to find out the suitable ratio of maize stover and concentrate for optimum growth, production, nutrient utilization and carcass characteristics in growing Nellore X Deccani ram lambs. A significant (P<0.05) difference in the ADG was noticed in ram lambs fed three experimental complete rations. There was 44.43 and 17.06 per cent higher gain in ram lambs fed MS50 ration in comparison to those fed MS70 (P<0.05) and MS60 (P>0.05) rations, respectively. The FCR was better (P>0.05) by 11.72 and 17.39 per cent, respectively with MS60 and MS70 rations in comparison to MS50 ration. The cost ()/kg live weight gain was comparable among the three rations but was numerically higher in ram lambs fed ration MS50 followed by MS60 and MS70 rations. No significant difference was observed among the three experimental complete rations in the digestibility coefficients of DM, OM, CP and NFE. The EE digestibility in MS60 (P>0.05) and MS50 (P<0.05) was higher by 4.77 and 8.30 per cent, respectively in comparison to MS70 ration. The CF digestibility in MS60 (P>0.05) and MS50 (P<0.05) rations was higher by 12.18 and 22.13 per cent, respectively in comparison to MS70 ration. The apparent digestibility coefficients of NDF, ADF and cellulose were comparable (P>0.05) among the three complete rations and the digestibility of hemicellulose was significantly (P<0.05) different among the rations. The per cent DCP was significantly (P<0.05) higher in MS50 than MS60 and MS70 rations. Whereas, the per cent TDN was either significantly (P<0.05) or non-significantly (P>0.05) higher in MS50 ration than MS60 and MS70 rations. The N balance and N absorbed (g/d) were significantly lower in lambs fed MS60 and MS70 rations than those fed MS50 ration. The N balance was linearly increased with increased level of concentrate. Among all the rations, the nitrogen balance was significantly (P<0.05) higher in MS50 ration, but there was no significant difference between MS60 and MS70 rations. The daily DCP and TDN intake (g/d) was significantly (P<0.05) higher in ram lambs fed MS50 ration in comparison to those fed MS60 and MS70 rations. The DCP intake ( g/kg w0.75) was significantly (P<0.05) different among the ram lambs fed MS70, MS60 and MS50 rations, the values (g/d or g/kg w0.75) being higher in lambs fed MS50 ration than those offered MS60 and MS70 rations. The intake of DCP (g/d or g/kg w0.75) was not significantly different between the lambs fed MS60 and MS70 rations. The TDN intake (g/d) was also significantly (P<0.05) different among the lambs fed MS70, MS60 and MS50 rations and the intake was significantly higher in the lambs fed MS50 ration followed by lambs fed MS60 and MS70 rations. However, TDN intake was not significantly different between lambs fed MS60 and MS70 complete rations. The live body weight and empty body weight was significantly (P<0.05) different among the experimental ram lambs fed three complete rations and was lower in lambs fed MS70 ration compared to those fed MS60 and MS50 rations. The hot carcass weight was significantly (P<0.05) different among the ram lambs fed experimental complete rations. However, no difference was observed in the animals fed MS60 and MS50 complete rations. There was no significant difference in dressing percentage either on live weight or empty body weight basis. The proportion of lean (%) was significantly higher (P<0.05) in MS50 complete ration than MS60 and MS70 rations, but was comparable between MS60 and MS70 complete rations. No significant variation could be seen in bone and fat (%) among the experimental rations. Increased (P>0.05) trend in bone meat ratio in carcasses was observed as the level of concentrate increased in the ration of experimental ram lambs. The moisture, protein and ash contents of meat were not affected and there was a significant (P<0.05) increase in fat (%) as the level of concentrate increased in maize stover based complete rations with different roughage to concentrate ratio. Based on the results of present study, it is concluded that maize stover, a potential cereal crop residue hitherto unused can be incorporated as roughage component in complete rations (mash form) at 50-60 per cent for obtaining optimum growth with better feed efficiency in growing Nellore X Deccani ram lambs. Varying levels of stover and concentrate in complete rations did not affect the digestibilities of nutrients barring fat and fibre in growing ram lambs. Level of inclusion of maize stover in complete rations has no effect on dressing percentage either on live weight or empty body weight basis.ThesisItem Open Access SUB-ACUTE TOXICITY STUDIES ON UNPROCESSED AND PROCESSED PONGAMIA PINNATA SEED CAKE IN RATS(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2013-02) SIRISHA, K; KALA KUMAR, B.D.P(MAJOR); GOPALA REDDY, A; MADHAVA RAO, T; ANJANEYULU, YABSTRACT : The present study was aimed to evaluate the toxic and protective effects of unprocessed and processed Pongamia pinnata seed cake in rats, which were divided into 3 groups as follows: Group 1: sham control, group 2: unprocessed Pongamia pinnata seed cake included at the level of 9% in the feed (toxic control) and group 3: processed (solvent extracted-isopropyl alcohol) Pongamia pinnata seed cake (detoxified cake) included at the level of 9% in the feed. Average body weights were recorded at weekly intervals and on 28th day, organs were collected for estimation of TBARS, protein carbonyls and GSH in kidney, liver and testes homogenates and estimation of epididymal sperm count from testes collected. Sero-biochemical parameters like ALT, total proteins & globulins, total cholesterol, HDL & LDL cholesterol, creatinine and LDH were estimated at fortnight intervals. Haemotological parameters (RBC, WBC, Hb and PCV) were also estimated at fortnight intervals. Serum troponins, PHA assay and testicular LDH were estimated at the end of the experiment. Histopathology of heart, liver, kidney, spleen and testis was also studied at the end. Mean body weight gain, GSH, total proteins and globulins, PHA assay and sperm count were significantly (P < 0.05) decreased in toxic control group (group 2), while TBARS, protein carbonyls, serum LDH, intra-testicular LDH, serum ALT, creatinine, total cholesterol and serum troponins were significantly (P < 0.05) increased in group 2. There was no significant difference in TEC, TLC, Hb, PCV, HDL cholesterol, LDL cholesterol, mean body weight gain (in the 1st week) in group 2. Group 1 did not reveal any abnormalities on histopathology. Group 2 showed interfibrillar haemorrhages, congestion and edema with disruption of cardiac myofibres in heart, marked degenerative changes in tubular epithelial cells and marked dilatation of tubules in kidney, marked central vein congestion and marked bile duct hyperplasia in liver, congestion and thickening of trabecular arteries in spleen and finally marked congestion and edema with disrupted cell wall in seminiferous tubules of testes. Group 3 showed mild lesions in heart, kidney, liver, spleen and testis. From this study, it is concluded that unprocessed Pongamia pinnata seed cake induces toxicity to heart, kidney, liver, spleen and testes, and group 3 showed restoration in all the parameters studied, suggesting reduced toxic potential of processed seed cake.ThesisItem Open Access ANATOMICAL STUDIES ON HAIR OF INDIAN SPOTTED DEER (Axis axis), BLACKBUCK (Antelope cervicapra) AND ASIAN ELEPHANT (Elephas maximus)(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2012-12) VINAYA SHEELA, S; PURUSHOTHAM, G(MAJOR); PRAMOD KUMAR, D; LAKSHMAN, MABSTRACT : The present study was undertaken on hair samples of Spotted deer, Black buck and Asian Elephant since they are considered as endangered species. Further the former two species are listed under Indian Wild life Act 1972. Six animals were selected from each species from Nehru Zoological park. Hair samples from six different regions viz., neck, back, lateral abdomen, forelimb, hind limb and tail were collected from each animal and processed for microscopic physical observations, cast technique for scale pattern and for SEM (scanning electron microscope) studies. Microscopic structure of hair of spotted deer and black buck comprised of cuticle, cortex and medulla from without inwards. Hairs of tail and back region of elephant had a cortex, cuticle and whereas all other regions lacked a typical medulla. Color variation was observed between the hairs of different species within an individual and also within an individual hair. Color of hairs collected from six different regions varied from colorless to blackish brown in spotted deer, colorless to dark brown in black buck and from light yellowish brown to dark brown in elephant. Tips of hair shafts in spotted deer were frayed in neck, abdominal and tail regions and were blunt in rest of the regions. In black buck hair tips were frayed in neck region, rounded in hind limb region whereas it was pointed in other regions. In elephant, hair tip was rounded in all regions except in the back and forelimb where hair tips were broken. The cuticle was a translucent colorless outer structure of hair shaft but light brown in hair of neck and back regions in spotted deer and in neck of black buck hair. In elephant hair it was colorless in all regions with light blackish brown margin. Smooth cuticular pattern throughout the length of hair was noticed in most of the regions of three species. Serrated cuticle was seen in the tip of forelimb hair in spotted deer, throughout the length of hair in tail region and mid shaft of forelimb in blackbuck. In back region of elephant it was wavy throughout the length. Cross sectional shape of hair shaft of spotted deer varied from oval to round. It was bean shaped in black buck but was rod shaped in the tip, oval, triangular or round near to base of the shaft in hair of tail region and round in the base of neck. Cross sections of hair in elephant were round. Cortex pattern was smooth throughout the length of hair in spotted deer whereas in black buck it was coarse in the tip and mid shaft of abdomen hair. It was coarse in the hair of all regions in elephant except for tip hairs of neck region. Cortical fusi were present in the proximal part of hair in tail region of spotted deer and back region of blackbuck. No ovoid bodies were observed in hair of spotted deer but were seen towards the base of the shaft of back, hind limb region hair in black buck and in the tip of the hair in abdomen and hind limb hair of elephant. Medulla was lattice type in spotted deer hair whereas it was non lattice type in black buck hair. Vacuolated medulla was evident towards base of the shaft in the hair of tail region in black buck. Medulla was absent in the tip and was tapered towards tip of the shaft in spotted deer and black buck hair. Medulla was not evident in the hair of elephant from all regions except in back and tail hair where multiple medulla was noticed. It was fragmentary or trace like towards tip of the shaft in spotted deer and black buck and its margins were scalloped in spotted deer. Scalloped, irregular and straight medullary margins were present in black buck hair. Wine glass shaped tapered medulla, fragmentary or widened towards base of the shaft were seen in spotted deer and black buck hair. Variation in pigment distribution was evident within individual hair of spotted deer and black buck. In former it was uniform, medial and random while in black buck it was uniform and banded and in elephant it was uniform. A significant difference of mean cortical thickness, medullary diameter, and medullary index of hair between spotted deer and blackbuck was noticed. Shaft diameter of three species differed significantly not only between species and different body regions but also within an individual hair. SEM studies revealed difference in scale pattern in the tip, mid shaft and base of an individual hair in spotted deer and black buck. Imbricate scale pattern with overlapped scales were present in the mid shaft and coronal type in the tip of the shaft. They were faint and distantly placed towards base of the shaft, but were compact in the elephant hair. Margins of the scales were smooth to slightly rippled in mid shaft region and towards the base of the shaft in spotted deer whereas it was smooth in black buck and rippled in elephant hair. Trough on surface of hair was an important feature of blackbuck hair that made it easy to differentiate from that of spotted deer hair. Number of scales per 100 μm length of hair was more at the tip in spotted deer (21.55-22.50) and blackbuck (18.05-20.52). In general scale width was more in the base of the shaft and diameter of mid shaft was more than base. Imbricate scale cast pattern was seen in hairs of spotted deer and black buck towards mid shaft and base. Hair tip showed coronal pattern in spotted deer and black buck. Scale cast imprints were not amenable in shaft of elephant hair which indicates adherence of scales to the shaft surface.ThesisItem Open Access EFFECT OF IRRADIATION ON QUALITY ATTRIBUTES AND SHELF LIFE OF CHICKEN EMULSION(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2012-12) RAVI, T; SHASHI KUMAR, M(MAJOR); SUDHAKAR REDDY, K; KRISHNAIAH, N; MUTHUKUMAR, MABSTRACT: A study was conducted to evaluate the effect of irradiation on shelf life of chicken meat emulsion stored at refrigerated temperature under vacuum package with addition of natural and chemical antioxidants. The overall mean pH of control samples were significantly lower than irradiated samples. The pH decreased with increased storage period. The overall mean 2-TBARS of control groups recorded significantly higher 2-TBARS when compared to treatment groups. Among treatment group, T2 recorded significantly lower 2-TBARS. The mean 2-TBARS values on day 35 were significantly higher than other storage days. The control group had significantly higher total plate counts, psychrotropic counts and lactobacillus counts than treatment group. No significant difference was noticed among treatment group in total plate counts, psychrotropic counts and Lactobacillus counts. A significant increase in all the bacterial counts was observed with increase in storage length. The mean E.coli and Salmonella counts (cfu/g) showed a gradual increase from day 1 to day 35 in control while no colonies could be detected in irradiated groups. The overall mean scores of control samples for appearance and colour, flavor, juiciness, texture and overall acceptability were significantly lower than treated samples. No significant difference in the sensory attributes was observed among the irradiated samples. The scores were lower on day 35 when compared to other days of storage. Thus, the present study indicated the promising potential of irradiation as a safe and efficient preservation method.