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Author: SAILAJA RANI, P × End date: 2009 × Start date: 2009 × Type: Thesis ×
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    ThesisItemOpen Access
    COMPARATIVE EVALUATION OF ANTI-INFLAMMATORY ACTIVITY AND SAFETY OF ALCOHOLIC EXTRACT AND ETHYL ACETATE FRACTION OF Ocimum sanctum IN RATS
    (Sri Venkateswara Veterinary University, TIRUPATI – 517 502,A.P, 2009-10) SAILAJA RANI, P; ADILAXMAMMA, K (Major); MADAN MOHAN, E; ESWARA PRASAD, P
    ABSTRACT : The methanolic extract and ethyl acetate fraction of Ocimum sanctum (Family: Labiatae) were investigated for anti-inflammatory activity at the dose rate of 90 and 45 mg/kg, respectively in the carrageenan (acute) and cotton pellet-induced granuloma (chronic) models in rats. Toxicity potential was also evaluated for the extracts in sub-acute toxicity model following oral administration. The methanolic extract and ethyl acetate fraction of Ocimum sanctum exhibited significant (P<0.05) anti-inflammatory effect at the doses tested and also significantly reduced the activities of ALT and AST against carrageenan-induced paw edema and cotton pellet-induced granuloma model, while the concentration of TBARS was significantly (P<0.05) reduced and the concentration of GSH was significantly (P<0.05) increased in granuloma model. The methanolic extract (450 mg/kg and 900 mg/kg) and ethyl acetate fraction (225 mg/kg and 450 mg/kg) were further evaluated for their toxicity potential by administering them orally for 28 days in rats. At the end of the experiment, haematological, sero-biochemical, oxidant-antioxidant profile and histological studies were conducted. The administration of methanolic and ethyl acetate fraction did not affect any alterations in the parameters assessed including body weights, haematology and biochemistry, except mild histological changes in visceral organs. The animals did not exhibit untoward signs of toxicity. The results revealed that methanolic and ethyl acetate fraction of Ocimum sanctum exhibited significant anti-inflammatory activity in the tested models and the extracts are non-toxic at the doses tested.