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Author: NAVYA, M × End date: 2024 ×
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    ThesisItemOpen Access
    INVESTIGATION OF INTERCONNECTION BETWEEN ANTIMICROBIAL RESISTANCE TO BIOCIDES AND ANTIBIOTICS IN Staphylococcus SPECIES ISOLATED FROM BUFFALOES
    (SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2021-04) NAVYA, M; ANAND KUMAR, P (MAJOR); RAMANI PUSHPA, N; BINDU KIRANMAY, CH
    The present study is undertaken to investigate the interconnection between antimicrobial resistance to biocides and antibiotics in Staphylococcus species isolated from buffaloes and to know the distribution of these genetic determinants in the isolates. Based on culture on mannitol salt agar and conventional biochemical tests (catalase test, oxidase test and coagulase test) 73 samples (60.84%) out of 120 collected in the present study were provisionally confirmed for Staphylococcus species. In PCR test with Staphylococcus genus specific primers 16S rRNA, all the 73 samples yielded PCR product of 228 bp. In PCR test with S. aureus specific oligonucleotide primers Staur 4 and Staur 6, 19 out of 73 Staphylococcus species isolates yielded specific PCR product of 1250 bp. Certain Staphylococcus species (other than S. aureus) are found to be coagulase negative staphylococci (CoNS). The S. aureus isolate P1 is a mannitol non-fermenter, but positive for coagulase production. With regard to antibiotic sensitivity of 73 Staphylococcus species isolates in in vitro antibiotic sensitivity test (AST) with antibiotic discs, majority isolates were sensitive to Gentamicin (n=71, 97.26%) followed by Amoxicillin/Clavulanic acid (n=70, 95.89%), Chloramphenicol (n=68, 93.15%) and Enrofloxacin (n=63, 86.30%), but highly resistant to Oxacillin (n=64, 87.67%). The Multiple Antibiotic Resistance index results with all the Staphylococcus species isolates, including S. aureus showed that a total of 31 samples (42.46%) showed the MAR index below 0.2 and remaining 42 samples (57.53%) showed the MAR index ≥ 0.2. In PCR test for detecting the genetic determinants of antibiotic and biocide resistance, out of 73 isolates of Staphylococcus species (including S. aureus) a total of 44 (60.27%) were blaZ positive, 42 (57.53%) were mecA positive, 32 (43.83%) were tetK positive, 6 (8.22%) were qacA/B positive and 5 (6.85%) were smr positive. In Staphylococcus species other than S. aureus, the coexistence of genetic determinants of biocide and antibiotic resistance were detected only in 3 isolates viz. M5, M7 and S11. In S. aureus isolates the coexistence of genetic determinants of biocide and antibiotic resistance were detected only in 5 isolates viz. M9, M10, N4, N5 and N6. In conclusion, perhaps for the first time the genetic determinants of biocides and antibiotic resistance in Staphylococcus species isolated from buffaloes is investigated in Andhra Pradesh. However, further detailed investigation is required with a greater number of samples from different settings.