Thumbnail Image

Thesis

Browse

20191
Reset filters
Author: DEEPTHI, CHANDAKA × Type: Thesis × Thesis Type: M.V.Sc. ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    MITOCHONDRIAL DIVERSITY AND PHYLOGENETIC STATUS OF PUNGANUR CATTLE
    (SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517502. (A.P.) INDIA, 2019-11) DEEPTHI, CHANDAKA; MURALIDHAR, M (MAJOR); VINOO, R; SUDHAKAR, K
    Cattle in India played an important role in the rural economy as supplementing family incomes and generating gainful employment in the rural sector, particularly among the landless laborers, small and marginal farmers. Among all the cattle breeds the dwarf breeds are more preferred among farmers as they can be maintained with less fodder, high disease resistant ability, high survivability in adverse environmental conditions and optimum milk production. Punganur is one such dwarf breed present in Andhra Pradesh. According to the available literature, the Punganur cattle were crossed with Mysore cattle and Kerry breed with time. Punganur cow has become a status symbol in recent years as wealthy livestock farmers began buying it, due to its cultural value. However, the present day Punganur cattle description was not in accordance with the available literature. With this in mind the objective of the present research is to study the genetic variation and phylogenetic status of Punganur cattle. using mitochondrial DNA D-loop region. Blood samples were collected from 28 unrelated Punganur animals which were further grouped as 14 Punganur animals and 14 Punganur type animals based on literature available. Unrelated Blood samples were also collected from Ongole, Hallikar, Vechur, Malnad Gidda and Bargur. Genomic DNA was isolated and 1024 bp mitochondrial D-loop region was amplified and sequenced. Sequence of Indian cattle breeds were obtained from NCBI database and total of 212 sequences were aligned and trimmed to have a final length of 912 bp. The measures like polymorphism, divergence, gene flow and genetic differentiation were estimated using DnaSP software. A phylogenetic tree was constructed using Neighbour joining method by MEGA software. The tree topology was tested using 1000 bootstrap replications. The results of polymorphism and divergence analysis disclosed the existence of high within breed polymorphism compared to between breed polymorphism which is in accordance with other cattle breeds of India. The findings of gene flow and genetic differentiation estimates concluded the presence of low differentiation and presence of gene flow between Punganur and Punganur type animals. The phylogenetic analysis indicated that, there is no differentiation between Punganur and Punganur type animals.