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Author: BUJJI, BANDARU × Start date: 2000 ×
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    ThesisItemOpen Access
    HEMATOBIOCHEMICAL PROFILE AND ELECTROPHORETIC PATTERN OF SERUM PROTEINS IN PUNGANUR CATTLE DURING DIFFERENT SEASONS
    (SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2021-07) BUJJI, BANDARU; ASWANI KUMAR, K (MAJOR); Prasada Rao, T; Srinivasa Prasad, CH
    A total of sixty apparently healthy Punganur cattle aged two years and above grouped as: Females (n=30) and Males (n=30) maintained at Livestock Research Station, SVVU, Palamaner, Chittor district were selected to study hemato-biochemical profiles and to compare the electrophoretic pattern of serum proteins over different seasons.THI findings during the study period suggests that the summer season was having moderate heat stress (79.03±1.22) on Punganur cattle. Hematological parameters such as Hb, TEC, PCV, MCV, MCH, MCHC, Monocytes, Granulocytes, Lymphocytes and Platelets did not show any significant change among all groups between seasons. WBC values were high during summer in males and herd while there was no change in females. Total Protein and globulins were high during summer while albumins and A/G ratio showed higher values during winter in all the groups. BUN and Creatinine concentration were high in summer compared to other seasons, but the values were within normal range. Blood glucose concentration was low in summer in all the groups while there was no change in Total Bilirubin. Total Cholesterol, Triglycerides and HDL cholesterol concentrations were significantly high in all the groups during summer than in winter and monsoon. No significant difference could be observed in the values of LDL cholesterol. High Sodium values with lower Mg was observed in winter among all the groups of animals. There was no significant difference in Serum Calcium and Phosphorus levels. High AST, ALT and ALP enzyme concentrations during summer with no alterations in GGT was observed in all the groups. SDS PAGE pattern of all the animals during all the seasons showed same pattern with six prominent bands at 22, 27, 40, 53, 66 and 130 kDa. 2D-PAGE analysis of serum protein samples of the Punganur cattle showed More than 415 spots. The present study revealed presence of 12, 23 and 21 differentially expressed protein spots in males; 18, 39 and 37spots in females between summer vs monsoon, summer vs winter and monsoon vs winter respectively.