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ThesisItem Open Access CHARACTERIZATION OF Staphylococcus aureus ISOLATES OF ANIMAL ORIGIN(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2003-11) ARUNA, VEDAVALLI; DHANA LAKSHMI, K(MAJOR); JANAKIRAMA SARMA, B; ANJANEYULU, YABSTRACT : The present investigation was carried out to isolate and characterize S. aureus from different animal and poultry species. Characterization was done by examination of the isolates for simultaneous coagulase and manitol fermentation, DNase, hemolysin, dermonecrotoxin production and antibiogram. The isolates were also subjected to finger printing analysis by REP and BOX-PCR. A total of 50 samples were collected from different animal species viz., buffalo, dog, goat, poultry and emu for isolation of S. aureus. The number of isolates from 14 buffalo milk samples,13 from birds, 13 from dogs and 10 from goats were 8, 6, 10 and 7, respectively. Identification of isolates by simultaneous testing of coagulase and mannitol fermentation was less time consuming and a convenient method. Most of the coagulase positive strains showed thermonuclease activity in toluidine blue DNA agar. Out of all the hemolysis positive isolates only one isolate from Emu bird exhibited dermonecrosis in rabbits. The RF for metronidazole was 100% and was more than 50% for ampicillin, penicllin-G, nitrofurantoin, amoxycillin and streptomycin. The RF of 25 – 50% was noticed for kanamycin and erythromycin. For doxycylcline, tetracycline, trimethoprim norfloxacin and cephalexin the RF was less than 25%. Out of 31 DNA samples of S. aureus analyzed by PCR technique, 14 samples produced PCR amplified products in REP and BOX and they were grouped into 6 major clusters in REP and 10 in BOX. There was extensive variability in DNA finger prints of S. aureus indicating differences in the genetic make up of S. aureus strains without any consideration for the animal source.