Thumbnail Image

Thesis

Browse

20161
Reset filters
Author: ANIL KUMAR, P ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    INFLUENCE OF LEPTIN ON THE EXPRESSION OF AROMATASE GENE IN CULTURED OVARIAN FOLLICLES OF SHEEP
    (SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2016-11) ANIL KUMAR, P; Veerabramhaiah, K(MAJOR); Siva Kumar, A.V.N; MuthaRao, M
    ABSTRACT: The expression of P450 aromatase gene was studied in the cumulus cells and oocytes isolated from : (i) In vivo grown preantral, early antral, antral, large antral follicles and COCs obtained from large antral follicles and subjected to 24 h of IVM and (ii) Preantral Follicles exposed to TCM 199, TCM 199 + Leptin (10 ng/ml) and standard medium + Leptin (10 ng/ml) media for 3 minutes, cultured in vitro for two, four or six days and subsequently matured in vitro for 24 h in respective cultures separately. Aromatase gene expression was observed at all the stages of in vivo grown ovarian follicles in both cumulus cells and oocytes and a decrease in the expression from PFs’ to early antral follicles followed by an increase in the antral follicles.The expression in cumulus cells and oocytes obtained from PFs’ cultured in TCM 199 and TCM 199 + Leptin supplemented medium was observed only in a few stages and the expression was significantly lower than the in vivo or standard + Leptin group. While the expression of the same in the cumulus cells in standard + Leptin medium in different stages was comparable to the expression of aromatase gene in in vivogrown ovarian follicles. But in oocytes the expression was significantly lower at all the stages in Standard + Leptin supplemented group compared to in vivo grown follicles. Estradioland Progesterone concentrations in the spent medium collected from PFs’ cultured in TCM 199, TCM 199 + Leptin and Standard + Leptin media showed an increasing trend from PFs’ to COCs obtained from six day cultured PFs’ and matured in vitro for 24 h. Estradiol concentration in Standard + Leptin medium showed a significant increase between the PFs’ and two day and six day cultured PFs’ and COCs obtained from six day cultured PFs’ matured in vitro for 24 h. Estradiol levels did not differ among similar stages except the COCs matured in vitro for 24 h between TCM 199 and TCM 199 + Leptin and TCM 199 + Leptin and Standard + Leptin. Progesterone levels in different media at similar stages showed no significant difference in spent medium collected between PFs’ and two day cultured follicles but in spent medium collected from four day, six day and COCs from six day cultured follicles after IVM showed significantly higher levels of progesterone in standard + Leptin supplemented medium. It may be concluded that, the supplementation of Leptin in the culture medium for sheep PFs’ is able to support the expression of aromatase gene in cumulus cells and oocytes. But the expression levels are not on par with the expression in in vivo grown follicles. Supplementation of Leptin @10 ng/ml enhances E2 and P4 concretions but it was not significant.