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1997 - 199912010 - 20111
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Subject: Veterinary Physiology × End date: 2015 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    AMELIORATION OF HEAT STRESS INDUCED OXIDATIVE DISTURBANCES BY HERBAL ANTIOXIDANTS IN BROILER CHICKEN
    (SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2011-04) SWATHI, B; Gupta, P.S.P(MAJOR); Nagalaxmi, D; Rajasekhar Reddy, A; RaJu, M.V.L.N
    ABSTRACT: A study was petformed to evaluate the antioxidative potential of herbals Tulsi (Ocimum sanctum) and Turmeric (Curcuma longa) in combating heat stress with two batches of broiler chicks in hot summer months. Each batch consisted of a total of 108 one day OM Vencobb broiler chicks which were grouped and offered 9 types of dietary regimens wtth variable concentrations and combinations of antioxidants. A group of 12 chicks was raised separately in stress free environment on basal diet without any antioxidant supplementation as control. Body weights and feed intake were measured at weekly intentab. The blood samples were colleded at 4" and 6" wk of age and haernatological estimations (RBC, WBC, Hb. & PCV) were carried out within 2 hrs of collection. While serum samples were preserved at -20°C until utilized for estimation of protein profiles (TP, albumen, globulin concentrations and A:G ratios), serum cortisol concentrations, humoral and cell mediated immune responses. Plasma samples were prepared by centrifugation, stored and utilized for analysis of enzymatic and non enzymatic antioxidant concentration. At the end of trail, breast and meat samples were procured by excision of sacrificed birds for studying organoleptic properties and lipid peroxidation. Histopathological studies of liver, bursa, brain and spleen were conducted to observe the tissue changes at the end of the experiment. The performance parameters (Body weights and feed efficiency), haernatologoical parameters (RBC, Hb. PCV and WBC), enzymatic (GSH-PX, Catalase and SOD), non enzymatic (Reducedglutathione) antioxidant concentrations, immunological indices (HI titers and CMI responses) were signifiintly low (PsO.01) in heat stressed birds compared to control and antioxidant supplemented groups. However, WBC count, serum cortisol concentrations, serum ALP and ALT activities were significantly (PS0.01) higher in heat stressed birds compared to control group. Histopathological study evidenced degenerative changes and pathological lesions in tissues collected from heat 8tmssed birds, While no lesions of pathological importance were observed with the tissues d control group. Sensory chamderistics of breast meat samples from heat stressed birds scored low on 5 point hedonic scale (3.42 for colour, 3.12 for texture, 3.42 for juiciness, 3.22 for tlavour and 3.14 for overall acceptability) compared to higher scores recorded for meat samples of control group. Dietary supplementation with herbals Tulsi and Turmeric was proved beneficial in terms of broiler performance, haematological, serological values. immunological indims and antioxidant status. Their inclusion also contributed to low levels of serum cortisol and improved sensory characteristics and reduced lipoperoxidative damage of broiler meat. Of the two herbals employed for the study. Turmeric had contributed to hgher body weights (1629.17 g and 1627.50 g with 0.2% and 0.4% level of inclusions, respectively), which were comparable with the weights achieved by supplementing the diet either with vitamin E alone (1637.52 g) and its combination with Selenium (1656.67 g) end control group (1684.17 g) at the end of 6'" week, Feed efficiency though low in the initial stages, an accelerated trend was noticed from 4m wk onwards wrth Turmeric supplementation. And even sensory attributes showed higher sensory scores with Turmeric supplementation at either of the dose. While, Tulsi, contributed to significantly (Ps0.01) higher concentration of enzymatic and non enzymatic antioxidants, blood and serum variables, and immunological indices at either of the dose supplemented. Herbals at higher concentration (Ocimum at 0.5% and Turmeric at 0.4%) had shown improved antioxidant -8. However, the two herbals when given in combination at different doses could not yield any additive effect. The benefdal results obtained with the supplementation of herbals at different doses were however remained low when compared to the values obtained with the diets inclusive of vitamin E alone, or its combination with Se and control group. But, considering the cost of these synthetic antioxidants vitamin E and Se and maintaining the birds in stress free environment, the herbals employed Tulsi and Turmeric in this study may be suggested as natural and economical alternatives in amelioration of heat stress and achieving higher performance
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    ThesisItemOpen Access
    Molecular Identification Of Opioid Receptors From Bovine Brain Tissue
    (SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 1997-01) Sastry, K.V.H; Yadgiri, B(MAJOR); Reddy, J.M; Janardanasarma, M.K; Somasekhar reddy, K