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Subject: Veterinary Physiology × Author: Siva Kumar, A.V.N. × End date: 2019 × Start date: 2010 ×
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    ThesisItemOpen Access
    INFLUENCE OF LEPTIN ON IN VITRO DEVELOPMENT OF PREANTRAL FOLLICLES OF SHEEP
    (SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2014-11) Siva Kumar, A.V.N.; Rao, V.H(MAJOR); Punya Kumari, B
    ABSTRACT : The role of human and ovine leptin on in vitro development of preantral follicles (PFs’) isolated from sheep ovaries was studied. Experiment I, was conducted to standardize the optimum concentration of human leptin for the growth of PFs’. The parameters set for the evaluation of the growth of PFs’ were increase in diameter, proportion of PFs’ exhibiting growth, antrum formation and maturation of oocytes to M II stage. All these parameters were higher in the leptin (10ng/ml) supplemented medium. Experiment II was conducted with the optimum level of leptin (10ng/ml) derived in experiment I to evaluate the effect of human or ovine leptin @10ng/ml in combination with various growth factors viz., TCM 199 B with FSH, T4, IGF-I and GH. Two controls viz., TCM 199 B and standard medium i.e., TCM 199 B supplemented with FSH (2ug/ml), T4 (1μg/ml), IGF-I (10ng/ml) and GH (1mIU/ml) were used. In the standard medium supplemented with human leptin, the proportion of PFs’ exhibiting growth (66.14±3.9), average increase in the diameter (11.1±0.87 μ), antrum formation (51.5±5.33) and maturation of oocytes to M II stage (24.1±3.7) were higher compared to the standard medium and standard medium supplemented with ovine leptin (10ng/ml). But there was no significant difference between the standard medium and the standard medium supplemented with ovine/human leptin. It is concluded that (i) the optimum dose of leptin for the growth of sheep PFs’ in vitro was 10ng/ml and (ii) human or ovine leptin supported similar growth of PFs’ cultured in the standard medium.