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Subject: Veterinary Physiology × Author: NIRUPAMA .M.U.L × Start date: 2010 ×
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    ThesisItemOpen Access
    GENE EXPRESSION PATTERNS OF SELECTIVE MONOCARBOXYLATE TRANSPORTERS IN BUBALINE SPERMATOZOA
    (SRI VENKATESWARA VETERINARY UNIVERSITY, TIRUPATI - 517 502. (A.P.) INDIA, 2023-10) NIRUPAMA .M.U.L; SRINIVASA PRASAD .CH (MAJOR); IQBAL HYDER; ASWANI KUMAR .K; MUTHA RAO .M
    The objective of the present study was to evaluate the gene expression of certain monocarboxylate transporters in bubaline spermatozoa. Eight adult healthy buffalo bulls between three to six years of age were randomly selected from ABC Semen Station, Veeravalli, Krishna District, Andhra Pradesh. The fresh semen samples were collected from eight buffalo bulls once in a week for four weeks by AV method. As a preliminary quality check, fresh semen samples (4 samples from each bull, n=32) were evaluated at the semen station for volume, concentration, mass motility, individual progressive motility, viability, HOST, acrosomal integrity and the mean±SE values were found to be 4.09±0.26mL, 1243.875±65.621 million/mL, 3.86±0.34 and 70.04±9.92%, 73.42±0.49%, 73.98±0.56% and 87.75±0.52% respectively. With respect to evaluation of gene expression of monocarboxylate transporters, the fresh semen samples were transported at 37°C. The samples were divided in to two groups. Fresh uncapacitated as control (4 samples from each bull n=32) and fresh capacitated (n=32). It was taken care that fresh semen samples had sperm concentration of 50-60 million spermatozoa. In vitro capacitation of fresh semen samples was performed in BO media supplemented with heparin for 2 hrs. Genes such as SLC16A1 and SLC16A7 were selected for the study. The mRNA isolated from respective samples were converted to cDNA and subjected to q-PCR analysis. The target genes were normalized to endogenous control GLUT5. The mRNA expression of genes in fresh capacitated was analyzed relative to fresh uncapacitated as control. Fold change in gene expression was calculated using ∆∆Cq method. Significant difference between groups for fold change was analyzed using t test and multiple comparison was performed by Duncan multiple range test. No significant difference (p<0.05) was observed in the mRNA expression of MCT1and MCT2 between fresh capacitated and fresh uncapacitated (control) semen samples. It was concluded that, in vitro capacitation induces no significant change in the mRNA expression of both SLC16A1 and SLC16A7 genes which encode for MCT1 and MCT2 respectively and MCTs exert minimal role in capacitation related biochemical changes in bubaline spermatozoa.