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20121
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Subject: Veterinary Microbiology × End date: 2012 × Start date: 2012 × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    CHARACTERIZATION OF AN INDIAN ISOLATE OF BLUETONGUE VIRUS SEROTYPE 16
    (SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2012-03) YOGANAND, BRUNDAVANAM; NARASIMHA REDDY, Y(MAJOR); DHANALAKSHMI, K; NAGENDRA HEGDE, R
    ABSTRACT: Bluetongue is an arthropod-borne disease, transmitting to ruminants through the bites of Culicoides. Bluetongue is listed as a ‘notifiable disease’ by the Office International des Epozooties (OIE), causing severe hemorrhagic disease with fever, lameness, coronitis, edematous lips and tongue and death. Export of animals and animal products from Bluetongue endemic countries like India are seriously being hampered. Bluetongue virus (BTV) belongs to the family Reoviridae, genus Orbivirus. Out of 24 serotypes identified worldwide, 12 serotypes have been reported by isolation and 11 serotypes by serology in India. VP2 gene is highly variable and responsible for neutralization and serotype specificity of BTV. Characterization of isolates which are definitively sero-typed is very much essential for developing suitable vaccine to control the disease in a given geographical region. This research work is focused on the characterization of an Indian isolate (VJW784) of bluetongue virus serotype 16, by RT-PCR and molecular techniques. This is the first time to document the complete sequencing of protein coding region of VP2 gene of an Indian isolate of bluetongue virus serotype 16. The VJW784 isolate was propagated on BHK-21 cell lines and harvested. dsRNA extracted from BHK-21 cells was used for testing by RT-PCR with serotype specific primers of BTV 1, 9, 10, 16, 21 and 23. Further serotype-specific primers for VP2 gene of BTV 16 were designed and used for specific amplification of segment 2. Cloning was carried out using pDK101 vector. The positive plasmid DNA and PCR amplicons were sequenced. The sequence information was aligned with DNAstar software and BLAST analysis was carried out. The sequence information provided herein may help to determine the geographic origin of VJW784isolate and defined the phylogenetic relationship of this isolate to other BTV strains.