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20193
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Subject: Veterinary Gynaecology and Obstetrics × End date: 2019 × Start date: 2019 ×
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    ThesisItemOpen Access
    STUDIES ON EFFICACY OF GnRH AND hCG DURING MID LUTEAL STAGE OF THE CYCLE IN REPEAT BREEDER GRADED MURRAH BUFFALOES (Bubalus bubalis)
    (SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517502. (A.P.) INDIA, 2019-04) VENKATESWARLU, M; SADASIVA RAO, K(MAJOR); SRINIVAS, M; DEVI PRASAD, V
    The present investigation entitled “Studies on efficacy of GnRH and hCG during mid luteal stage of the cycle in repeat breeder Graded Murrah buffaloes (Bubalus bubalis)” was taken up by screening 130 repeat breeder lactating buffaloes to study the causes of repeat breeding. The efficacy of GnRH (Group 1 – 10μg of GnRH 8 hours before AI at observed estrus and Group 2 – 10μg of GnRH 8 hours before AI at observed estrus and 13th day of post insemination) and hCG (Group 3 – 1500 IU of hCG 8 hours before AI at observed estrus and Group 4 – 1500 IU of hCG 8 hours before AI at observed estrus and 13th day of post insemination) protocols for enhancement of conception rates were studied in 60 repeat breeding Graded Murrah buffaloes in comparison with normal cyclical buffaloes as untreated control (Group 5). The causes of repeat breeding in Graded Murrah buffaloes reared under rural climatic condition includes, repeat breeders without any palpable abnormalities in the genital tract, subclinical endometritis and tubal blockage was recorded to be 60, 66, 4 buffaloes and the percentage was 46.15, 50.78, 3.07, respectively among 130 repeat breeder buffaloes examined. White side test and PSP dye test could be effectively used to diagnosis the subclinical endometritis and tubal blockage, respectively. The mean length of estrous cycle in repeat breeders and normal cyclical buffaloes was 21.68±0.39 and 22.60±0.31 days; duration of estrus was 22.10±0.33 and 24.39±0.08 hours; intensity of estrus 13.42±0.37 and 15.4±0.63 points, respectively. There was no significant difference (P<0.05) in length of the estrous cycle, whereas significantly (P<0.05) less in duration of estrus and lower intensity of estrus in repeat breeder buffaloes when compared with that of normal cyclical buffaloes. Repeat breeder Graded Murrah buffaloes exhibited lowered levels of mean haemoglobin (8.61±0.20 gm%), PCV (35.21±0.99 %) when compared to normal cyclic buffaloes. Further, biochemical parameters viz., mean serum glucose (48.26±2.83 mg/dl), calcium (7.21±0.18 mg/dl), phosphorus (4.44±0.12 mg/dl), total protein (6.57±0.09 g/dl) and cholesterol (85.57±1.90 mg/dl) concentration also showed lowered levels in repeat breeder buffaloes when compared to normal cyclical buffaloes. The conception rates of repeat breeding Graded Murrah buffaloes in Group 1, 2, 3, 4 and control group (Group 5) were recorded to be 40.00, 46.67, 53.33, 66.67 and 42.85 per cent, respectively. It was concluded that all the treatments except that of Group 1 could improve the conception in repeat breeder buffaloes compared with that of normal cyclical buffaloes. However, hCG treatment groups revealed higher per cent of conception rates (53.33 and 66.67%) when compared with that of GnRH treatment groups (40.00 and 46.67%). The human chorionic gonadotropin administered at observed estrus and on 13th day post insemination could effectively improve the conception rates in repeat breeding Graded Murrah buffaloes.
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    ThesisItemOpen Access
    STUDIES ON UTERINE TORSION AND CERVICAL CHANGES AFTER ITS CORRECTION IN GRADED MURRAH BUFFALOES (Bubalus bubalis)
    (SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517502. (A.P.) INDIA, 2019-03) BHAVANA, V; SRINIVAS, M(MAJOR); VENKATA NAIDU, G; LAKSHMI RANI, N
    In the present study analysis of dystocia in Graded Murrah buffaloes presented to Obstetrical ward during a two year period revealed a high prevalence of maternal dystocia (80.80%) over fetal dystocia (19.19%) was observed. The prevalence of uterine torsion was highest (95.62%) among the maternal dystocia and was 77.27 % among all the dystocia cases presented. Detailed obstetrical examinations were carried out in 148 buffaloes which exhibited signs of uterine torsion and were treated by using Modified Schaffer’s method. A total of 79 (53.38 per cent) buffaloes were detorted with 1-2 rotations, 53 buffaloes (35.81 per cent) with more than 2 rotations and 16 buffaloes (10.81 per cent) failed to respond for detorsion and were subjected to caesarean section. Buffaloes which responded for detorsion had a dilated cervix (46.97%), incompletely dilated cervix (43.94%) and closed and intact cervix (9.09%). The buffaloes which were successful for detorsion (n=35) were randomly divided into two groups, in which 15 buffaloes had completely dilated cervix and 20 buffaloes which had incompletely dilated cervix. Hemato-biochemical parameters of these buffaloes were compared with normally calved buffaloes (n=10). Hemogram revealed a significant (P<0.05) reduction in hemoglobin, packed cell volume, eosinophil, and lymphocyte counts in uterine torsion affected buffaloes with completely dilated and incompletely dilated cervix post detorison when compared to normally calved buffaloes. The total leucocyte, neutrophil and monocyte counts were significantly higher in buffaloes having completely dilated cervix, incompletely dilated cervix post detorsion as compared to normally calved buffaloes. Biochemical analysis revealed a significant (P<0.05) decrease in calcium, phosphorus, magnesium and MDA concentrations in buffaloes having completely dilated and incompletely dilated cervix post detorsion when compared to normally calved buffaloes. There was a significant (P<0.05) increase in glucose, β hydroxy butyric acid, creatinine phosphokinase, lactate dehydrogenase, and certain oxidative stress parameters like maloinaldehyde, superoxide dismutase, glutathione peroxidase concentrations in the uterine torsion affected buffaloes with completely dilated and incompletely dilated cervix post detorsion compared to eutocia buffaloes. Buffaloes with incompletely dilated cervix (n=58) post detorsion were randomly, subjected to two treatment protocols and cervical changes and efficacy of the treatment were recorded. Buffaloes of each group were further grouped based on the texture of the external os of cervix into grade 1 (smooth and soft cervix), grade 2 (partially lobulated and moderately soft cervix) and grade 3 (lobulated and hard cervix). The buffaloes in Group I are treated with Dextrose salines, Calcium Borogluconate, Oxytocin, Valethamate Bromide along with periodic massage with warm CMC gel and overall mean interval between treatment and complete dilatation of cervix was 229.60±10.69 minutes with a success rate of 86.21%. Group II buffaloes (n=29) were treated with Dextrose salines, Calcium Borogluconate, Oxytocin, Valethamate Bromide and massage with CMC gel in combination with Misoprostol tablet (PGE1) @ 1000 μg powdered and applied to the cervical lumen and subsequent hourly massages were done with CMC alone. The overall mean interval between treatment and complete dilatation of cervix was 203.13±5.41 minutes with a success rate of 100.00%. It was concluded from the present study that the therapeutic efficacy of Group II protocol was better as it required lesser number of massages, less mean interval (min) between treatment and complete dilatation of cervix with 100.00 per cent success rate. Further, it was evident that Misoprostol caused cervical softening and dilatation of the cervix without any side effects.
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    ThesisItemOpen Access
    STUDIES FOR AUGMENTATION OF FERTILITY BY HORMONAL TREATMENTS IN POSTPARTUM ANESTROUS ONGOLE COWS (Bos indicus)
    (SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517502. (A.P.) INDIA, 2019-01) SAHITHI, KONGARA; SADASIVA RAO, K(MAJOR); SRINIVAS, M; LAKSHMI RANI, N
    In the present study, treatment of postpartum lactating anestrous Ongole cows (Bos indicus) using Heatsynch and G6G hormonal protocols and their influence on fertility was studied. The mean level of PCV is statistically significant (P<0.05) on the day 0 and on the day of induced estrus. The total serum cholesterol, glucose and protein profiles varied non-significantly between conceived and non-conceived Ongole cows, in both the treatments. The mineral profile did not reveal significant variations (P>0.05) in serum calcium, phosphorus, iron, copper and zinc concentrations of Ongole cows between periods of the treatments with Heatsynch and G6G protocols. The estrus detection rate was 75.0 (6/8), and 87.5 (7/8) per cent in Heatsynch (Group A) and G6G (Group B) groups. Analysis of data of onset of estrus revealed significant difference (P<0.05) between two treatment groups. Heatsynch treated group showed intense to weak estrus signs, while the G6G treated group showed intense to intermediate estrus signs. Peak estrus synchrony with highest number of animals exhibiting estrus between 48 to 72 h with 4/6 in Heatsynch group and between 24 to 48 h with 4/7 in G6G group was observed. The artificial insemination submission rate was 75.0 (6/8) and 87.5 (7/8) per cent in Heatsynch and G6G groups, respectively. The conception rate at induced estrus with Heatsynch treatment was 66.67 (4/6), and with G6G treatment it was 71.43 (5/7) per cent. Overall conception rate among the postpartum lactating anestrous Ongole cows treated was higher with 85.71 (6/7) per cent in G6G (Group B) followed by 83.33 (5/6) per cent in Heatsynch (Group A). During this treatment period in the control group, only 37.5 (3/8) and 25.0 (2/8) per cent anestrous Ongole cows exhibited estrus and conceived, respectively. The results of present study, with inseminations at induced estrus appears as an effective way of managing reproduction in postpartum lactating anestrous Ongole cows using Heat synch and G6G protocols which revealed acceptable estrus response and conception rates.