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Subject: Veterinary Anatomy × Author: BINDU, MOTUPALLI × End date: 2013 × Start date: 2013 ×
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    ThesisItemOpen Access
    HISTOLOGICAL AND HISTOCHEMICAL STUDIES ON THE COAGULATING AND PREPUTIAL GLANDS OF ALBINO RAT (Rattus norvegicus)
    (SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI – 517 502. (A.P) INDIA, 2013-11) BINDU, MOTUPALLI; KISHORE, P V S (Major); RAJU, N. K. B; SREENU, MAKKENA; NAGA MALLESWARI, Y
    ABSTRACT : The study was undertaken on the light microscopic and ultra structure of the coagulating and preputial glands. These were the two additional paired male accessory sex glands in the rat. For this study 10 albino rats were used. The coagulating glands of either side were located along the inner curvature of the seminal vesicles. Each gland formed a single narrow duct that opened into the urethra alongside the duct of the seminal vesicle. The gland was compound tubular. The secretory units and the ducts were lined by simple cuboidal or low columnar epithelium which projected into the lumen and formed folds inside the compartments. The mode of secretion was both merocrine and apocrine. Ultrastructural study revealed secretory cells of different electron density. The cytoplasm also contained the secretory granules and the vacuoles of different sizes towards the luminal side which showed the surface microvilli. A weak PAS positive reaction was observed in the gland. A positive reaction for proteins was observed in the cytoplasm of the secretory cells and in the luminal fluid. No reaction for lipids was observed. A mild acid phosphatase activity was observed in the secretory cells located peripherally. A very weak alkaline phosphatase activity was observed towards the luminal side in the epithelial cells of the secretory units and the ducts. The preputial glands were small and elongated flask shaped structures on either side of the penis in the male. They were compound alveolar glands of the holocrine type. Each acinus increased in size with the continued storage of fatty substances. Small , rounded acini with a layer of flattened peripheral cells, enlarged acini composed of hypertrophied, vacuolated cells and acini with cellular degeneration and a distinct lumen, several of which fused to form large masses of disintegrating cells were also observed. The above states of activity represented the cycle of sebum formation. The gland was not associated with the hair follicle. The main excretory duct emptied into the area between the inner surface of the prepuce and glans penis. Ultrastructural study revealed cells of different sizes in different phases of secretory activity. The cytoplasm contained several small secretory granules and small to large lipid droplets. Well developed endoplasmic reticulum along with numerous golgi complexes were observed. The large cells had spherical nuclei with evenly dispersed euchromatin along with peripheral heterochromatin. A PAS positive reaction was observed in most regions of the gland. It showed only a slight decrease in reactivity but remained almost similar upon treatment with saliva. Fine protein granules were observed in the smaller acinar cells and large sized granules were observed in the central acinar cells approaching degeneration. Abundant lipid droplets were observed throughout the acinar cells. A mild acid phosphatase activity was observed in some of the acinar cells. A weak alkaline phosphatase activity was observed in the secretory acini that were in active phase of secretion.