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  • ThesisItemOpen Access
    STUDIES ON DIETARY SUPPLEMENTATION OF PROBIOTICS IN CULTIVABLE FISHES
    (SRI VENKATESWARA VETERINARY UNIVERSITY , TIRUPATI - 517 502 , A.P, 2014-03) GANAPATHI, CHELPURI; CHAMUNDESWARI DEVI, B(Major); RAVINDRA KUMAR REDDY, D; BALASUBRAMANIAN, A
    ABSTRACT: Fish nutrition has advanced dramatically in recent years with the development of new, balanced commercial diets that promote optimal fish growth and health. Feed accounts for a major part (30-70%) of the total operation cost of an average fish farm. Traditionally, animal protein sources, particularly fishmeal have been the major ingredient of aqua feeds. Fishmeal is one of the most expensive ingredients in formulated fish feeds. Due to increasing demand, decreasing supply and the high cost of fish meal, fish nutritionists have concentrated their efforts to find alternative protein sources to substitute fish meal in the diet of fish. There are reports of growth promotion and improved health when probiotic were used. The present studies on “Studies on Dietary Supplementation of Probiotics in cultivable fishes” was carried out at Fisheries Research Station, Kakinada. Probiotics containing Bacillus spp. and Lactobacillus spp. was used in the diets at four different levels 0.2%, 0.4%, 0.6% and 0.8%. Control diet was prepared with soybean meal (SBM), groundnut cake (GNC) , de oiled rice bran (DOB), fish meal and maize. All the formulated diets were is nitrogenous with 30% crude protein. Proximate composition and mineral composition of diets were analyzed by AOAC (1995) methods. Each diet was fed to Mullet and rohu in triplicate tanks twice daily at 5% average body weight of the fish for three months for determination of growth response, survivability and health. Fortnightly sampling was done for the estimation of the water quality parameters and growth parameters [growth, percentage of survival, feed conversion ratio (FCR), protein efficiency ratio (PER), Specific growth rate (SGR); gut microflora were estimated at the end of the experiment. Isolation of gut probiotics in the intestine of experimental fishes was conducted at the end of the experiment. Fishes were analyzed for proximate composition at the beginning as well as at the end of the experiment. Significant difference was observed in growth, FCR and PER in both Mullet and Rohu. Good survival rate was observed in mullet . No mortality was observed in Mullet and Rohu . Inclusion levels of Probiotic at 0.2% , 0.4%,0.6% and 0.8% effected microflora in the intestine of only Mullet . Carcass composition was observed to be better at Probiotic 0.2%, 0.4%, 0.6% and 0.8% in Mullet and Rohu respectively. Better growth performance was observed at 0.8% of Probiotic level of inclusion in Mullet. However, no significant differences were observed in the growth, and survival at inclusion level of 0.4% and 0.8% Probiotic in Mullet. Better performance was observed at 0.2% and 0.4% level in Rohu. Hence, it can be recommended that Probiotic can be included at 0.8% and 0.4% level of probiotic in the diets of Mullet and Rohu respectively.