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20212
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Subject: Biochemistry × Start date: 2017 ×
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    ThesisItemOpen Access
    HEMATOBIOCHEMICAL PROFILE AND ELECTROPHORETIC PATTERN OF SERUM PROTEINS IN PUNGANUR CATTLE DURING DIFFERENT SEASONS
    (SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2021-07) BUJJI, BANDARU; ASWANI KUMAR, K (MAJOR); Prasada Rao, T; Srinivasa Prasad, CH
    A total of sixty apparently healthy Punganur cattle aged two years and above grouped as: Females (n=30) and Males (n=30) maintained at Livestock Research Station, SVVU, Palamaner, Chittor district were selected to study hemato-biochemical profiles and to compare the electrophoretic pattern of serum proteins over different seasons.THI findings during the study period suggests that the summer season was having moderate heat stress (79.03±1.22) on Punganur cattle. Hematological parameters such as Hb, TEC, PCV, MCV, MCH, MCHC, Monocytes, Granulocytes, Lymphocytes and Platelets did not show any significant change among all groups between seasons. WBC values were high during summer in males and herd while there was no change in females. Total Protein and globulins were high during summer while albumins and A/G ratio showed higher values during winter in all the groups. BUN and Creatinine concentration were high in summer compared to other seasons, but the values were within normal range. Blood glucose concentration was low in summer in all the groups while there was no change in Total Bilirubin. Total Cholesterol, Triglycerides and HDL cholesterol concentrations were significantly high in all the groups during summer than in winter and monsoon. No significant difference could be observed in the values of LDL cholesterol. High Sodium values with lower Mg was observed in winter among all the groups of animals. There was no significant difference in Serum Calcium and Phosphorus levels. High AST, ALT and ALP enzyme concentrations during summer with no alterations in GGT was observed in all the groups. SDS PAGE pattern of all the animals during all the seasons showed same pattern with six prominent bands at 22, 27, 40, 53, 66 and 130 kDa. 2D-PAGE analysis of serum protein samples of the Punganur cattle showed More than 415 spots. The present study revealed presence of 12, 23 and 21 differentially expressed protein spots in males; 18, 39 and 37spots in females between summer vs monsoon, summer vs winter and monsoon vs winter respectively.
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    ThesisItemOpen Access
    A STUDY ON SOME OXIDATIVE-STRESS AND ANTIOXIDANT MARKERS IN PUNGANUR CATTLE DURING DIFFERENT SEASONS
    (SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2021-05) SHALOME, SANDRAPATI; ASWANI KUMAR, K (MAJOR); ESWARA PRASAD, P; SRINIVASA PRASAD, CH
    Zebu cattle are reported to be more heat tolerant than crossbred cattle. It is hypothesised that the oxidative status of zebu and crossbred cattle might be compromised differentially to heat stress. Environmental conditions vary significantly during different seasons in tropical climate. The present study was conducted in three different seasons, (summer, Monsoon and winter) in sixty (60) apparently healthy Punganur cattle aged 2 years and above, maintained at Livestock Research Station, SVVU, Palamaner, Chittoor with an objective to assess changes in different markers of oxidative stress, HSP 70 and associated hormones. The mean THI values in the present study were observed to be significantly (p<0.05) higher during summer (79.03±1.22) than monsoon (70.93±1.17). The mean concentrations of glucose values (mg/dl) in males, females and total herd in the present study were significantly low in summer compared to monsoon and winter. Heat stressed cows become hypersensitive to insulin, and will reduce or block adipose mobilization and increasing glucose ‘burning’ in an attempt to minimize metabolic heat production The mean values of TBARS, Catalase (CAT), Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in males, females and total herd were found to be significantly (P<0.05) higher in summer season compared to monsoon and winter. The activity of Glutathione-S-Transferase in males, females and total herd was found to be insignificant in all the three seasons that were studied. The reduced glutathione values in males, females and total herd were found to be significantly higher in summer (P<0.05) compared to monsoon and winter. The concentration of serum Heat Shock Protein 70 (HSP 70) in males, females and total herd were significantly (p<0.05) higher in summer compared to monsoon and winter, indicating Cellular response of the animals to stressors. The mean concentration values of serum cortisol in males, females and total herd of Punganur cattle were significantly (p<0.05) higher in summer compared to monsoon and winter. Serum triiodothyronine (T3) and Thyroxine (T4) activity in males, females and total herd of Punganur cattle showed a significantly lower (p<0.05) levels in summer compared to monsoon and winter. The trends of T3 and T4 hormones in the present study during different seasons indicates the altered metabolism during different seasons.