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  • ThesisItemOpen Access
    EFFECT OF SELECTED HERBS ASHWAGANDHA (WITHANIA SOMNIFERA) AND SHATAVARI (ASPARAGUS RACEMOSUS) AS DIETARY SUPPLEMENTS ON GROWTH AND METABOLIC RESPONSES OF LITOPENAEUS VANNAMEI (BOONE, 1931)
    (SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2020-10) SRINIVASA RAO, APPANA; RAVINDRA KUMAR REDDY, D (MAJOR); CHANDRASEKHARA RAO, A; DHANAPAL, K; SUGUNA, T
    The present study on “Effect of selected herbs Ashwagandha (Withania somnifera) and Shatavari (Asparagus racemosus) as dietary supplements on growth and metabolic responses of Litopenaeus vannamei (Boone, 1931).” was conducted in the Wet Laboratory of the Department of Aquaculture, College of Fishery Science, Sri Venkateswara Veterinary University, Muthukur for a period of 127 days (including acclimatization period of 36 days). The experimental shrimps, Litopenaeus vannamei were fed on the diets containing Ashwagandha and Shatavari at 0.05%, 0.10% and 0.15% individually and in combination. The control diet is without Ashwagandha or Shatavari. The control and experimental diets were prepared to have almost similar crude protein level of 35% using the ingredients such as fish meal, shrimp meal, soya meal, deoiled rice bran, ground nut oil cake as major ingredients. The control and experimental tanks were maintained to have 15 ppt salinity water. Total experiment contained 9 treatments and one control maintained in triplicate. Important water quality parameters (Dissolved oxygen, Temperature, pH, Total ammonia and Nitrite), Growth and Survival were recorded at weekly intervals. The immunological parameters such as pro phenoloxidase, lysozyme and total serum/plasma protein were anlaysed at 60 days and 91 days of the experimental period. The digestive enzymes protease, amylase and lipase activity was analyzed at the end of the experimental period. The highest average body weight gain of 18.82 gm ±1.574 was recorded in the treatment AS1 that contained combination of Ashwagandha 0.05% + Shatavari 0.05% in diet and the lowest average body weight gain of 13.64 gm ±0.895 in control (C). The overall highest survival of 100% in AS1 and A1 and lowest survival of 73.33% in control (C) was recorded. There is significant difference in weight gain, Specific Growth Rate, Feed Conversion Ratio and Protein Effeciency Ratio for L. vannamei fed with various levels of Ashwagandha and Shatavari incorporated diets compared to control diet fed group. The immunological parameters 2 such as pro-phenoloxidase, Lysozyme & total serum/plasma protein and the digestive enzymes protease, amylase and lipase showed significant increase in all the treatment groups containing Ashwagandha and Shataavari in diet compared to the group fed with control diet. The Treatment AS1 that contained both Ashwagandha and Shataviari extracts each @ 0.05% was found significantly superior among all the treatments. Both Ashwagandha and Shatavari extracts showed better performance in terms of growth, digestive enzymes and immunological responses individually as well as in combination at rate of 0.05% inclusion level in the diet for Litopenaeus vannamei. Therefore these findings clearly indicates that inclusion of Ashwagandha and Shatavari extracts in shrimp feed will have positive benefits in increasing growth as well as immunity and approximately 27% extra profit to the marginal and small scale farmer in Litopenaeus vannamei.