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Subject: Aquaculture × Author: PRAMODA MALLI, G J × End date: 2016 ×
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    ThesisItemOpen Access
    EVALUATION OF COTTONSEED MEAL AND ACETES INDICUS MEAL AS REPLACEMENTS TO FISH MEAL IN LITOPENAEUS VANNAMEI DIETS REARED UNDER VARYING WATER SALINITIES
    (SRI VENKATESWARA VETERINARY UNIVERSITY , TIRUPATI-517502, Andhra Pradesh, 2014-12) PRAMODA MALLI, G J; RAVINDRA KUMAR REDDY, D (Major); HARIBABU, P; SUDHAKAR, O
    ABSTRACT: The present study “Evaluation of Cottonseed meal and Acetes indicus meal as replacements to fish meal in Litopenaeus vannamei diets reared under varying water salinities.” was conducted in the Wet Laboratory of the Department of Aquaculture, College of Fishery Science, Sri Venkateswara Veterinary University, Muthukur. Cottonseed meal, (CSM) Acetes indicus (AI) meal, and Fish meal (FM) were used at three different protein levels (25%, 30% and 35%). Shrimp diet was prepared by mixing all the ingredients in required proportions, ground and sieved to the required size and made in to pellets. Studies were conducted to evaluate the replacement of fish meal with cottonseed meal and Acetes indicus meal at three different salinities 5ppt, 15ppt and 30ppt salinities. The experiments were conducted for 63 days in aquaria. Triplicates were maintained for each of the treatment. Feed was given three times a day. Weekly sampling was done for water quality parameters viz., pH, Temperature, Dissolved Oxygen (DO), Total alkalinity and Total hardness. The growth, survival and feed conversion ratio were estimated by sampling the shrimp once in a week. The DO was found to be in the range of 5.02ppm and 8.42ppm at 5ppt, 6.29ppm to 8.52ppm at 15ppt and 7.2ppm to 8.99ppm at 30ppt salinity. The pH ranged from 8.0 to 8.3 at 5ppt, 8.1 to 8.3 at 15ppt and 8.3 to 8.5 at 30ppt, during the period of experiment. The range of total alkalinity varied between 150 mg/l to 340 mg/l at 5ppt salinity, 612mg/l to 632 mg/l at 15ppt salinity and 660mg/l to 68mg/l at 30ppt salinity. Total hardness of water ranged between 225 mg/l to 237 mg/l at 5ppt salinity, 660mg/l to 684 mg/l at 15ppt salinity and61mg/l to 743mg/l at 30ppt salinity. The growth performance of L. vannamei was found to be best when fed with diet AI30 (11.05gm) and the least with CSM25 (9.95gm) at 5ppt salinity, AI30 (12.01gm) and CSM25 (10.01gm) at 15ppt salinity, AI30 (12.22gm) and CSM25 (10.00gm) at 15ppt salinity. L. vannamei fed on diet AI30 showed the highest average weight gain of 1.38gm by the 63rd while the FM25 recorded the lowest weight gain of all the treatments (1.15gm) at 5ppt salinity.AI30 (2.02gm) and CSM25 (1.12gm) recorded the highest and lowest average weight gain at 15ppt salinity.AI30 (2.21gm) and CSM35 (0.41gm) recorded highest and lowest average weight gain at 30ppt salinity. Specific growth rate was highest (3.159) in shrimps fed with diet AI30, and least (2.930) in the CSM25 at 5ppt salinity. In 15ppt salinity, Specific growth rate was highest (3.302) in shrimps fed with diet AI30, and least (2.960) in the CSM25. Similarly, Specific growth rate was highest (3.287) in shrimps fed with diet AI30, and least (2.969) in the CSM25 at 30ppt salinity. Survival rates were best in shrimps fed on FM30 and FM35, which showed 78% survival rate at 5ppt salinity, FM35 showed 88% survival rate at 15ppt salinity and FM30 and FM35 showed 85% survival rate at 30ppt salinity at the end of the experiment. The shrimps fed with CSM25 and CSM30 diets showed the least survival rate (65-75%). The shrimps fed on FM30 diet showed the best FCR of 1.66 followed by FM30 with an FCR of 1.72. The shrimps fed with CSM25 showed the least response with an FCR of 2.95 From the above studies, it can be concluded that Cottonseed meal and Acetes indicus meal can be included in the diets of L. vannamei up to 25% protein inclusion for the total replacement of fish meal.