Loading...
Thesis
Browse
Search Results
ThesisItem Open Access HAEMATOLOGICAL, BIOCHEMICAL AND ENDOMETRIAL HISTOMORPHOLOGICAL STUDIES IN POSTPARTUM EWES(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2006-06) ANJAIAH, V; MOULI KRISHNA, K(MAJOR); VENU GOPAL NAIDU; SRI LATHA, Ch; MOHAN NAIDU, GThesisItem Open Access EFFECT OF GnRH ON CONCEPTION RATE IN REPEAT BREEDER GRADED MURRAH BUFFALOES(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2012-06) VENKAT RAO, B; CHANDRA SHEKAR REDDY, K(MAJOR); SADASIVA RAO, K; PURUSHOTHAM, GABSTRACT: The present investigation “Effect of GnRH on conception rate in repeat breeder graded Murrah buffaloes” was taken up on 86 repeat breeder buffaloes to study the incidence of repeat breeding and various aspects of estrous cycle. The efficacy of GnRH protocols (10μg or 2.5 ml, 20μg or 5.0 ml given 8 hours before AI and on day 12 post AI either once or twice in different treatment groups) for enhancement of fertility was studied in 70 repeat breeding graded Murrah buffaloes. The estrous cycle length in six treatment groups was 21.6±0.69, 20.1±0.43, 20.3±0.58, 21.5±0.34, 21.3±0.54, and 20.2±0.47. The estrus duration in six treatment groups were 23.8±0.36, 24.5±0.31, 23.4±0.48, 24.2±0.47, 23.6±0.40, and 24.3±0.30 and the estrus intensity were 13.2±0.36, 14.1±0.28, 13.7±0.21, 14.1±0.31, 13.1±0.31, 13.2±0.25 days in group 1, group 2, group 3,group 4, group 5, group 6, respectively. In control groups the length of estrous cycle, duration of estrus and intensity of estrus were 21.5±0.60 days, 22.2±0.36 hours and 12.9±0.18 points, respectively. Normal estrus pattern was observed in 87.20 per cent of repeat breeder buffaloes. The repeat breeder buffaloes were randomly divided into seven groups (each group consisting of 10 buffaloes). The repeat breeding buffaloes in group 1 and 2 were treated with 10 μg or 2.5 ml and 20 μg or 5 ml of GnRH (Buserlin) 8 hours before AI, respectively. In group 3 and 4 were treated with 10 μg or 2.5 ml and 20 μg or 5 ml of GnRH on day 12 post AI respectively. Group 5 were treated with 10 μg or 2.5 ml of GnRH (Buserlin) 8 hours before AI and on day 12 of post AI. Group 6 were treated with 20 μg or 5 ml of GnRH (Buserlin) 8 hours before AI and on day 12 of post AI. Group 7 were consisted of untreated control buffaloes. The conception rates of repeat breeding graded Murrah buffaloes in group 1, 2, 3, 4, 5, 6 and control group were found to be 50.00, 60.00, 40.00, 40.00, 50.00, 60.00 and 30.00 per cent, respectively. It was concluded that all treatments significantly (P < 0.05) enhanced the conception in repeat breeder buffaloes when compared to control buffaloes. The GnRH could be effectively used for higher conception rates in repeat breeding buffaloes.ThesisItem Open Access STUDIES ON ESTRUS SYNCHRONIZATION AND FOLLICULAR DEVELOPMENT PATTERN IN ONGOLE COWS(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2012-07) VENKATA RAMANA, K; Sadasiva Rao, K(MAJOR); Chandrasekhar Reddy, K; Raghavender, K.B.P; Gopal Reddy, AABSTRACT: The present investigation was aimed to study the follicular dynamics, serum progesterone concentration during postpartum and effect of GnRH and PGF2α analogues to induce estrus in postpartum anoestrus lactating Ongole cows to reduce the service period. In the ultrasound monitoring of present study in thirteen cows, follicles measuring above 6 mm diameter and corpus luteum measuring 8-10 mm were detected by day 20-25 postpartum period In the present study, the first and second wave of the dominant follicle emerged on day 1.80 + 0.8 and 12.46 + 0.20 days. Where as in three wave cycles first, second and third waves emerged on 1.52 + 0.03, 9.60 + 0.40 and 15.14 + 1.13 days of the estrous cycle, respectively. The mean follicle diameter of the dominant follicles determined by ultrasonography of the ovaries. The first and second wave pattern revealed that 10.23 + 0.40 and 12.30 + 0.36 mm in the two wave cycle. It was observed in the present study that the first wave dominant follicle of three wave cycles attained a maximum size of 10.70+0.20 mm with a mean growth rate of 0.87+0.03 mm per day. In the second wave of the dominant follicle maximum size was 9.80+0.37 mm with growth rate of 0.77+0.06 per day and the third wave maximum diameter was 12.80+0.37 mm with a mean growth rate of 1.46+0.90 mm per day, which is significantly (P< 0.05) bigger and faster in growth rate than the first and second waves in the same estrous cycle. However, the growth rate of second wave and third wave were not significant (P<0.05). In the present study, in natural cycle, the corpus luteum grew to a mean value of maximum diameter of 15.93 + 0.37 and 17.8 + 0.37 mm on the day of 13.1+1.50, 14.6+0.56 and remained up to 14.6+ 1.50 and 15.9+0.45 days of the estrous cycle in two and three wave cycles, respectively. The wave emergence initiated by the GnRH administration was recorded in the recent study was 1.33+0.21 days and the dominant follicle grew to the maximum diameter of the 12.48 + 0.57 mm with a growth rate of 1.66 + 0.26 mm per day. In the present investigation about 22.85 per cent (16/70) of the postpartum lactating Ongole cows have shown estrus by day 60 out of seventy postpartum cows. The postpartum onset of behavioral estrus was highly variable and varied from 25 – 195 days with a mean value of 109.50 + 4.66. Estrus was exhibited by 32.50 and 62.50 per cent cows during the day and night time respectively. In the present study, estrus behaviour was classified as intense, normal and weak. Only 18.75 per cent of cows exhibited intense and 18.75 per cent cows exhibited weak estrus and the remaining (62.50 per cent) cows showed normal estrus. In the present study, the mean estrus duration recorded in Ongole cows was 18.23 + 4.20 hours and the mean estrous cycle length was 21.50+0.21 days. In the present study, 56.25, 57.14 and 100.00 per cent cows required one, two or more artificial inseminations per conception. The mean time required for conception after calving was recorded as 163.60+10.72 (75 -200) days. The serum progesterone concentration increased steadily from day 0 to 10 in cyclic cows and further increased in fertile cows from day 17 to 25 after breeding and there was a sharp decline in progesterone levels between day 17 to 25 (estrus). Among the postpartum anoestrus cows synchronized with GnRH + PGF2α + GnRH (Group A) and PGF2α at 12 days interval (Group B) the reproductive performance of group B was found to be better. The mean conception rate was recorded as 46.15+0.21, 54.54 + 0.36 and 67.00+0.26 in natural estrus , group A and group B, respectively. The conception rate in natural estrus was significantly (P<0.05) lesser than induced groups. The overall mean time interval from parturition to conception in natural postpartum lactating Ongole cows in control (group C) was 163.60+ 10.72 days with a range of 75-200. Where as the same in treatment groups was 84.95+3.79 with a range of 70-117 in group A and 89.40 + 3.89 with a range of 70-115 days in group B. The statistical analysis of the data revealed that the service period in treatment groups was significantly (P<0.01) shorter than control group (Group C). From this study, it may be concluded that, treatment of postpartum cows at day 60 with GnRH and PGF2α enhances the fertility significantly (P<0.01) and reduces the service period.ThesisItem Open Access STUDIES ON THE CRYOPRESERVATION AND FERTILITY OF BOVINE SEMEN WITH BlOCIPHOS PLUS DILUTOR(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 1998-11) KARUNAKARA RAO, N; Seshagiri Rao, A(MAJOR); Subramanyam Naidu, K; Venkateswara Rao, NThesisItem Open Access STUDIES ON OESTROUS CYCLE IN CROSS-BRED PIGS (Large White Yorkshire x Indigenous)(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 1997) VENKATA RAMANA, KUDIKILLA; SESHAGIRI RAO, A(MAJOR); VENUGOPAL NAIDU, K; VENKATESWARA RAO, N; KRISHNA REDDY, NThesisItem Open Access STUDIES ON THE SEMEN CHARACTERISTICSS AND PRESERVATION IN NATIVE BUCKS WITH PARTICULAR REFERENCE TO PROSTAGLANDIN F2~ AND VITAMIN A TREATMENT(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 1993-07) VENKATESWARLU, A; VENKATAMUNI CHETTY, A(MAJOR); VENUGOPALA NAIDU, K; RAMA RAO, PThesisItem Open Access EFFECT OF MINERAL SUPPLEMENTATION IN POST PARTUM ANOESTRUS GRADED MURRAH BUFFALOES(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2012-07) GOPALA KRISHNA, M.V.; RAM CHANDRA REDDY, K(MAJOR); SADASIVA RAO, K; GIREESH KUMAR, V; KISHAN KUMAR, MABSTRACT: The present investigation ―Effect of mineral supplementation in post partum anoestrus graded Murrah buffaloes‖ was undertaken in post partum pluriparous true anoestrus buffaloes (above 2 months) after calving, having a body condition score ranging from 3.0 to 4.0 screened clinically for reproductive disorders. 60 animals free from reproductive problems and having completely involuted uterus were selected for the study. Animals were divided into 4 groups comprising 15 in each group and subjected to different mineral supplementation (Group-I: Cyclomin-7 at the dose rate of 2boli/week/animal and Dicalcium phosphate at the dose rate of 30 gm/day/animal, Group-II: Non-chelated minerals at the dose rate of 50 gm/day/animal, Group-III: Chelated minerals at the dose rate of 50 gm/day/animal, Group-IV: Control group, without any mineral supplementation) protocols for 30 days and were fed with dry fodder, green fodder and concentrates as per the production requirements in intensive feeding system. During the course of treatment estrus symptoms were monitored with the help of teaser bulls twice or thrice per day and by visual observation 5 to 6 times per day. Animals which were not showing estrus symptoms were examined once in a week per rectally to know the ovarian activity. Animals which showed estrus symptoms have been inseminated twice during late estrus period. After breeding, animals were examined for subsequent estrus cycle and inseminated if required during subsequent cycles. Pregnancy Diagnosis was done per rectally after 50 days of breeding to know the conception status. Blood samples have been collected on zero, 15th, 30th days of treatment for estimation of serum minerals and haematological parameters. Estrus intensity and duration of estrus has been recorded based on the physiological symptoms. The mean haemoglobin levels on zero, 15th and 30th day in group I were 11.05 ± 0.26, 11.31 ± 0.27 and 11.55 ± 0.27, in group II were 11.01 ± 0.22, 11.32 ± 0.23 and 11.63 ± 0.22, in group III were 11.03 ± 0.18, 11.46 ± 0.26, and 11.89 ± 0.27 and in group IV were 11.01 ± 0.16, 11.03 ± 0.17 and 11.06 ± 0.17 g% respectively. Haemoglobin levels on 15th and 30th day of treatment group III was significantly (p < 0.05) higher. In between groups there was no significant difference in haemoglobin levels on zero, 15th day. But on 30th day three treatment groups were having statistically similar concentrations which were significantly (p < 0.05) higher than the control group. The mean Serum calcium levels on zero, 15th and 30th day were 7.20 ± 0.23, 7.69 ± 0.23 and 8.08 ± 0.22 in group I, 7.15 ± 0.21, 7.85 ± 0.12 and 8.63 ± 0.16 in group II, 7.24 ± 0.23, 7.97 ± 0.12 and 8.79 ± 0.07 in group III and 7.31 ± 0.10, 7.34 ± 0.06 and 7.35 ± 0.07 mg/dl in group IV respectively. On 30th day of treatment the serum calcium levels were higher in group III followed by group II, group I and lowest in group IV. The mean Serum phosphorus levels on zero, 15th and 30th day in group I were 5.46 ± 0.29, 5.79 ± 0.32 and 6.24 ± 0.18 mg/dl, in group II were 5.78 ± 0.18, 5.87 ± 0.28 and 6.30 ± 0.25 mg/dl, in group III were 5.56 ± 0.07, 5.96 ± 0.17 and 6.94 ± 0.05 mg/dl and in group IV were 5.56 ± 0.07, 5.83 ± 0.11 and 5.80 ± 0.10 mg/dl respectively. On 30th day of treatment the serum phosphorus level was significantly (p < 0.05) higher in group III followed by group II, I and IV. The mean Serum iron levels on zero, 15th and 30th day in group I were 2.716 ± 0.112, 3.122 ± 0.084 and 3.180 ± 0.110, in group II were 2.656 ± 0.058, 3.147 ± 0.068 and 3.246 ± 0.140, in group III were 2.689 ± 0.047, 3.174 ± 0.194, and 3.680 ± 0.081 and in group IV were 2.679 ± 0.039, 2.690 ± 0.061 and 2.702 ± 0.060 ppm respectively. Serum iron levels in group I, II were significantly (p < 0.05) lower on zero day when compare to 15th and 30th day. In between four groups, on 30th day group III was having significantly (p < 0.05) higher Iron levels. The mean Serum copper levels on zero, 15th and 30th day were 0.047 ± 0.003, 0.097 ± 0.003 and 0.142 ± 0.006 ppm in group I, in group II were 0.049 ± 0.005, 0.127 ± 0.005and 0.163 ± 0.005, in group III were 0.052 ± 0.002, 0.149 ± 0.003 and 0.184 ± 0.005 and in group IV were 0.049 ± 0.002, 0.052 ± 0.002 and 0.054 ± 0.003 ppm respectively. The serum copper levels were significantly (p < 0.05) higher on 30th day followed by 15th day and lowest on zero day in all three treatment groups. In between four groups there was significant difference (p < 0.05) on 15th and 30th day and group III was having significantly (p < 0.05) higher serum copper levels. The mean serum manganese levels on zero, 15th and 30th day in group I were 0.056 ± 0.003, 0.076 ± 0.003 and 0.151 ± 0.007 ppm, in group II were 0.058 ± 0.002, 0.112 ± 0.002 and 0.165 ± 0.006 ppm, in group III were 0.054 ± 0.002, 0.148 ± 0.002 and 0.182 ± 0.002 ppm and in group IV were 0.056 ± 0.002, 0.056 ± 0.003 and 0.055 ± 0.003 ppm respectively. The serum manganese levels were significantly (p < 0.05) different in all three treatment groups on zero, 15th, 30th day. In between four groups there was significant (p < 0.05) difference on 15th and 30th day and group III was having higher serum manganese levels. The mean serum zinc levels on zero, 15th and 30th day in group I were 1.062 ± 0.023, 1.162 ± 0.033 and 2.639 ± 0.061, in group II were 1.089 ± 0.008, 1.248 ± 0.018 and 3.038 ± 0.017, in group III were 1.075 ± 0.010, 1.427 ± 0.039 and 3.767 ± 0.042 and in group IV were 1.032 ± 0.012, 1.050 ± 0.010 and 1.054 ± 0.010 ppm respectively. On 15th and 30th day group III was having significantly (p < 0.05) higher serum zinc level followed by II, I and IV. The percentage of estrus response was 46.66 per cent, 66.66 per cent, 86.66 per cent and 33 per cent; mean duration of estrus was 22.13 ± 0.83, 26.46 ± 0.98, 32.93 ± 0.81 and 15.80 ± 0.65 hours; the intensity of estrus was 10.57 ± 0.69, 12.30 ± 0.76, 13.15 ± 0.72 and 11.4 ± 1.36 points; the interval between treatments to the estrus exhibition 46.67 ± 0.95, 35.20 ± 1.06, 25.0.98 and 70.13 ± 1.79 days and conception rates were 20 per cent, 40 per cent, 73.33 per cent and 13.33 per cent in group I, II, III and IV respectively. Regarding estrus response, duration of estrus and estrus intensity there was significant (p < 0.05) difference in between all four groups and group III was having significantly (p < 0.05) higher and followed by group II, I and lowest in IV. The interval between treatments to the estrus exhibition there was significant (p < 0.05) difference in between all four groups, group III was having significantly (p < 0.05) lesser interval next higher in group II, I and highest in group IV. The conception rates were significantly (p < 0.05) higher in group III and followed by group II and I lowest in IV. Based on the results of this study come to the concluded that due to the supplementation of chelated mineral mixture, the mean serum mineral levels and bioavailability increased more than non chelated mineral mixture and Di Calcium phosphate plus Cyclomin – 7. Consequently it enhanced the estrus response, duration of estrus and estrus intensity and decreased the interval between the treatment to the estrus exhibition and increased the conception rate in post partum anoestrus graded Murrah buffaloes.ThesisItem Open Access STUDY ON THE EFFICACY OF CABERGOLINE AND PGF2 α IN THE TREATMENT OF CANINE PYOMETRA(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2011-10) BASANTI JENA; SADASIVARAO, K(MAJOR); CHANDRA SEKHAR REDDY, K; RAGHAVENDER, K.B.PThesisItem Open Access INCIDENCE OF UTERINE NEOPLASMS IN ABATTOIR GENITALIA OF BUFFALOES (Bubaius bublis)(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2011-09) ANUSHA, K; MOULI KRISHNA, K(MAJOR); VEERABRAHMAIAH, K; SRILATHA, ChABSTRACT: The objective of the present study was to s&ndardize the diagnostic procedure and asaxhh the incidence of u-e neoplasms in slaughter bopse genitalia. To fulm the objective, cytology* histopathology and imm- ' ' j wae studied. In the present study grossly no tumour mass was obsd in majority of ganitalia (98.63%). Of course, in oae case grossly neoplastic masses of small to big size were noticed (1.37%). Wbaogo in otha samples the cbmga obsemd wae small whitish foci over ~ldomctrium (821Ya), thickmad utabe wall (6.84%), congested atdometrim and mpmetrium (5.47%). petahbe ova claunJes (2.73%), cystic ddum(1 .37%). pmmmian oysts (2.73%) and purulent discharge (137.h). Cytological studies mealed almmnd cdlular pattan in 73.97% samples ~~@daofu n~~o plssiaW haaas, 26.03% gaait.lia pnsootcd normal apithelial cell pttan. Basad w ~tho10gical aadicp 52.05% @tab wen donned for xqhh, while 21.92% far dysplasia Cbrwic odomatritis was fi,md in 26.03% gsnitaIia. 'Ibe n40plmma obsmd ware (421 I%), lymphsarcoma with (23.68%). hammngiosamoma (7.90%) cand hacammggoma with (7.90%). Mocytic ad- and schhus ad-oma ltcmuntod b 25 d 12.5% mspsctivdy of all adamamrhoma samples. Whw, lymphosarooma with schhous dsnoouoiwma mted to 1 1.1 1 % of lymphosarcoma with adcolooatdwuna (23.68%). Other woph foumd ware leiomp-ma, myxosarcoma, carcinoaanwnna, ~opaadomietrial~ * sarccnm hneosmmna with and leiomyoma with adeaocarcinoma each in one out of 38 neoplasms (2.63%). The mean AgNOR wunts for the neoplasia, drsplasia and chronic endomehitis were 6.76, 5.05 and 3.08, respectively which differed significantly. Positive labeling for VEGF was observed in neoplasia. Finally it was concluded that in chronic infatile animals cytological and histopathological investigations could be considered in orda to arrive at uterine status, because very often red examin8tion might be arbitrary, erroneous and insensitive to detect all forms of uterine abnWties.