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Open Access
EFFECT OF GnRH ON CONCEPTION RATE IN REPEAT BREEDER GRADED MURRAH BUFFALOES
(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2012-06) VENKAT RAO, B; CHANDRA SHEKAR REDDY, K(MAJOR); SADASIVA RAO, K; PURUSHOTHAM, G
ABSTRACT: The present investigation “Effect of GnRH on conception rate in repeat breeder graded Murrah buffaloes” was taken up on 86 repeat breeder buffaloes to study the incidence of repeat breeding and various aspects of estrous cycle. The efficacy of GnRH protocols (10μg or 2.5 ml, 20μg or 5.0 ml given 8 hours before AI and on day 12 post AI either once or twice in different treatment groups) for enhancement of fertility was studied in 70 repeat breeding graded Murrah buffaloes. The estrous cycle length in six treatment groups was 21.6±0.69, 20.1±0.43, 20.3±0.58, 21.5±0.34, 21.3±0.54, and 20.2±0.47. The estrus duration in six treatment groups were 23.8±0.36, 24.5±0.31, 23.4±0.48, 24.2±0.47, 23.6±0.40, and 24.3±0.30 and the estrus intensity were 13.2±0.36, 14.1±0.28, 13.7±0.21, 14.1±0.31, 13.1±0.31, 13.2±0.25 days in group 1, group 2, group 3,group 4, group 5, group 6, respectively. In control groups the length of estrous cycle, duration of estrus and intensity of estrus were 21.5±0.60 days, 22.2±0.36 hours and 12.9±0.18 points, respectively. Normal estrus pattern was observed in 87.20 per cent of repeat breeder buffaloes. The repeat breeder buffaloes were randomly divided into seven groups (each group consisting of 10 buffaloes). The repeat breeding buffaloes in group 1 and 2 were treated with 10 μg or 2.5 ml and 20 μg or 5 ml of GnRH (Buserlin) 8 hours before AI, respectively. In group 3 and 4 were treated with 10 μg or 2.5 ml and 20 μg or 5 ml of GnRH on day 12 post AI respectively. Group 5 were treated with 10 μg or 2.5 ml of GnRH (Buserlin) 8 hours before AI and on day 12 of post AI. Group 6 were treated with 20 μg or 5 ml of GnRH (Buserlin) 8 hours before AI and on day 12 of post AI. Group 7 were consisted of untreated control buffaloes. The conception rates of repeat breeding graded Murrah buffaloes in group 1, 2, 3, 4, 5, 6 and control group were found to be 50.00, 60.00, 40.00, 40.00, 50.00, 60.00 and 30.00 per cent, respectively. It was concluded that all treatments significantly (P < 0.05) enhanced the conception in repeat breeder buffaloes when compared to control buffaloes. The GnRH could be effectively used for higher conception rates in repeat breeding buffaloes.
Open Access
EFFECT OF MINERAL SUPPLEMENTATION IN POST PARTUM ANOESTRUS GRADED MURRAH BUFFALOES
(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2012-07) GOPALA KRISHNA, M.V.; RAM CHANDRA REDDY, K(MAJOR); SADASIVA RAO, K; GIREESH KUMAR, V; KISHAN KUMAR, M
ABSTRACT: The present investigation ―Effect of mineral supplementation in post partum anoestrus graded Murrah buffaloes‖ was undertaken in post partum pluriparous true anoestrus buffaloes (above 2 months) after calving, having a body condition score ranging from 3.0 to 4.0 screened clinically for reproductive disorders. 60 animals free from reproductive problems and having completely involuted uterus were selected for the study. Animals were divided into 4 groups comprising 15 in each group and subjected to different mineral supplementation (Group-I: Cyclomin-7 at the dose rate of 2boli/week/animal and Dicalcium phosphate at the dose rate of 30 gm/day/animal, Group-II: Non-chelated minerals at the dose rate of 50 gm/day/animal, Group-III: Chelated minerals at the dose rate of 50 gm/day/animal, Group-IV: Control group, without any mineral supplementation) protocols for 30 days and were fed with dry fodder, green fodder and concentrates as per the production requirements in intensive feeding system. During the course of treatment estrus symptoms were monitored with the help of teaser bulls twice or thrice per day and by visual observation 5 to 6 times per day. Animals which were not showing estrus symptoms were examined once in a week per rectally to know the ovarian activity. Animals which showed estrus symptoms have been inseminated twice during late estrus period. After breeding, animals were examined for subsequent estrus cycle and inseminated if required during subsequent cycles. Pregnancy Diagnosis was done per rectally after 50 days of breeding to know the conception status. Blood samples have been collected on zero, 15th, 30th days of treatment for estimation of serum minerals and haematological parameters. Estrus intensity and duration of estrus has been recorded based on the physiological symptoms. The mean haemoglobin levels on zero, 15th and 30th day in group I were 11.05 ± 0.26, 11.31 ± 0.27 and 11.55 ± 0.27, in group II were 11.01 ± 0.22, 11.32 ± 0.23 and 11.63 ± 0.22, in group III were 11.03 ± 0.18, 11.46 ± 0.26, and 11.89 ± 0.27 and in group IV were 11.01 ± 0.16, 11.03 ± 0.17 and 11.06 ± 0.17 g% respectively. Haemoglobin levels on 15th and 30th day of treatment group III was significantly (p < 0.05) higher. In between groups there was no significant difference in haemoglobin levels on zero, 15th day. But on 30th day three treatment groups were having statistically similar concentrations which were significantly (p < 0.05) higher than the control group. The mean Serum calcium levels on zero, 15th and 30th day were 7.20 ± 0.23, 7.69 ± 0.23 and 8.08 ± 0.22 in group I, 7.15 ± 0.21, 7.85 ± 0.12 and 8.63 ± 0.16 in group II, 7.24 ± 0.23, 7.97 ± 0.12 and 8.79 ± 0.07 in group III and 7.31 ± 0.10, 7.34 ± 0.06 and 7.35 ± 0.07 mg/dl in group IV respectively. On 30th day of treatment the serum calcium levels were higher in group III followed by group II, group I and lowest in group IV. The mean Serum phosphorus levels on zero, 15th and 30th day in group I were 5.46 ± 0.29, 5.79 ± 0.32 and 6.24 ± 0.18 mg/dl, in group II were 5.78 ± 0.18, 5.87 ± 0.28 and 6.30 ± 0.25 mg/dl, in group III were 5.56 ± 0.07, 5.96 ± 0.17 and 6.94 ± 0.05 mg/dl and in group IV were 5.56 ± 0.07, 5.83 ± 0.11 and 5.80 ± 0.10 mg/dl respectively. On 30th day of treatment the serum phosphorus level was significantly (p < 0.05) higher in group III followed by group II, I and IV. The mean Serum iron levels on zero, 15th and 30th day in group I were 2.716 ± 0.112, 3.122 ± 0.084 and 3.180 ± 0.110, in group II were 2.656 ± 0.058, 3.147 ± 0.068 and 3.246 ± 0.140, in group III were 2.689 ± 0.047, 3.174 ± 0.194, and 3.680 ± 0.081 and in group IV were 2.679 ± 0.039, 2.690 ± 0.061 and 2.702 ± 0.060 ppm respectively. Serum iron levels in group I, II were significantly (p < 0.05) lower on zero day when compare to 15th and 30th day. In between four groups, on 30th day group III was having significantly (p < 0.05) higher Iron levels. The mean Serum copper levels on zero, 15th and 30th day were 0.047 ± 0.003, 0.097 ± 0.003 and 0.142 ± 0.006 ppm in group I, in group II were 0.049 ± 0.005, 0.127 ± 0.005and 0.163 ± 0.005, in group III were 0.052 ± 0.002, 0.149 ± 0.003 and 0.184 ± 0.005 and in group IV were 0.049 ± 0.002, 0.052 ± 0.002 and 0.054 ± 0.003 ppm respectively. The serum copper levels were significantly (p < 0.05) higher on 30th day followed by 15th day and lowest on zero day in all three treatment groups. In between four groups there was significant difference (p < 0.05) on 15th and 30th day and group III was having significantly (p < 0.05) higher serum copper levels. The mean serum manganese levels on zero, 15th and 30th day in group I were 0.056 ± 0.003, 0.076 ± 0.003 and 0.151 ± 0.007 ppm, in group II were 0.058 ± 0.002, 0.112 ± 0.002 and 0.165 ± 0.006 ppm, in group III were 0.054 ± 0.002, 0.148 ± 0.002 and 0.182 ± 0.002 ppm and in group IV were 0.056 ± 0.002, 0.056 ± 0.003 and 0.055 ± 0.003 ppm respectively. The serum manganese levels were significantly (p < 0.05) different in all three treatment groups on zero, 15th, 30th day. In between four groups there was significant (p < 0.05) difference on 15th and 30th day and group III was having higher serum manganese levels. The mean serum zinc levels on zero, 15th and 30th day in group I were 1.062 ± 0.023, 1.162 ± 0.033 and 2.639 ± 0.061, in group II were 1.089 ± 0.008, 1.248 ± 0.018 and 3.038 ± 0.017, in group III were 1.075 ± 0.010, 1.427 ± 0.039 and 3.767 ± 0.042 and in group IV were 1.032 ± 0.012, 1.050 ± 0.010 and 1.054 ± 0.010 ppm respectively. On 15th and 30th day group III was having significantly (p < 0.05) higher serum zinc level followed by II, I and IV. The percentage of estrus response was 46.66 per cent, 66.66 per cent, 86.66 per cent and 33 per cent; mean duration of estrus was 22.13 ± 0.83, 26.46 ± 0.98, 32.93 ± 0.81 and 15.80 ± 0.65 hours; the intensity of estrus was 10.57 ± 0.69, 12.30 ± 0.76, 13.15 ± 0.72 and 11.4 ± 1.36 points; the interval between treatments to the estrus exhibition 46.67 ± 0.95, 35.20 ± 1.06, 25.0.98 and 70.13 ± 1.79 days and conception rates were 20 per cent, 40 per cent, 73.33 per cent and 13.33 per cent in group I, II, III and IV respectively. Regarding estrus response, duration of estrus and estrus intensity there was significant (p < 0.05) difference in between all four groups and group III was having significantly (p < 0.05) higher and followed by group II, I and lowest in IV. The interval between treatments to the estrus exhibition there was significant (p < 0.05) difference in between all four groups, group III was having significantly (p < 0.05) lesser interval next higher in group II, I and highest in group IV. The conception rates were significantly (p < 0.05) higher in group III and followed by group II and I lowest in IV. Based on the results of this study come to the concluded that due to the supplementation of chelated mineral mixture, the mean serum mineral levels and bioavailability increased more than non chelated mineral mixture and Di Calcium phosphate plus Cyclomin – 7. Consequently it enhanced the estrus response, duration of estrus and estrus intensity and decreased the interval between the treatment to the estrus exhibition and increased the conception rate in post partum anoestrus graded Murrah buffaloes.
Open Access
Evaluation of superovulatory response in Sahiwal x Jersey crossbred cows with different doses of FSH
(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2013-01) PRIDHVIDHAR REDDY, Y.V; Veerabramhaiah, K(MAJOR); Mouli Krishna, K; Ravindra Reddy, Y
Open Access
MORPHOMETRIC EVALUATION OF CERVIX AND ITS INFLUENCE ON INTRACERVICAL PASSAGE OF INSEMINATION PIPETTE IN NATIVE SHEEP
(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI – 517 502. (A.P) INDIA, 2017-06) SATYA PRASAD, B; SRINIVAS, M(Major); VENKATA NAIDU, G; VAIKUNTA RAO, V
ABSTRACT: Morphometric evaluation of native sheep genitalia from slaughter house was carried out and biometry revealed a significant difference (P<0.01) was observed between the size of ovaries and uterus in ewe lambs and adult ewes where as no significant difference (P>0.05) was observed in these parameters between the adult ewes during follicular and luteal phases. The mean length (cm) and diameter (cm) of cervix of ewe lambs, adult ewes during follicular and luteal phases in present study were 2.94±0.13 and 0.81±0.05; 5.23±0.11 and 1.04±0.03 and 4.83±0.12 and 0.9±0.03 cm, respectively. The predominant type of external os in ewe lambs was papilla, while in adult ewes it was rose. The mean depth of penetration (cm) and number of cervical folds recorded in ewe lambs, adult ewes during follicular and luteal phases were 0.75±0.03, 3.10±0.21 and 0.87±0.05 and 3.30±0.12, 3.92±0.07 and 4.00±0.00, respectively. The occurence of grade 1 cervix was more in ewe lambs and adult ewes with least occurence of grade 3 while the occurence of grade 2 was intermediate with a similar trend for average depth of penetration for cervix. In the present study the measurements of the cervical moulds of ewe lambs and adult ewes revealed a significant difference (P<0.01) in the length (cm), weight (gm), number of folds, height of fold, diameter of fold (cm), internal diameter of fold (cm) and distance from os to folds (cm) between the ewe lambs and adult ewes. From the current study it was concluded that the alignment of the second or third cervical ring, in relation to the first ring was the principal determinant of depth of penetration and the major impediment to transcervical AI. Thus, it was opined that application of transcervical AI and ET could be practically possible at ages greater than 4 years, or at 3-4 years of age.
Open Access
COMPARATIVE EVALUATION OF VARIOUS METHODS OF EARLY PREGNANCY DIAGNOSIS IN GRADED MURRAH BUFFALOES (Bubalus bubalis)
(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI – 517 502. (A.P) INDIA, 2014-10) SRILATHA, BETHAPUDI; VENKATA NAIDU, G (Major); SRINIVAS, MANDA; DEVI PRASAD, V
ABSTRACT: The aim of the present investigation was to evaluate the different methods of early pregnancy diagnosis viz., Heat-aid rapid detection kit, Serum progesterone assay, Punyakoti test and Transrectal ultrasonography and to compare between and among different methods. The present study was conducted on 86 Graded Murrah buffaloes aged between 4 to 8 years showing estrus signs and were artificially inseminated upon rectal and ultrasonographic observations. These were randomly divided into four test groups and pregnancy diagnosis was carried out at an early stage from day 20 post insemination. Pregnancy diagnosis in Group 1 (Heat-aid kit) was carried out using qualitative commercial kit on day 21 post insemination with appearance of pink colour in nonpregnant and no colour change in pregnant buffaloes. Buffaloes in Group II (Serum progesterone assay) were analyzed for early pregnancy with estimation of serum progesterone concentration on day 21 post insemination using ELISA and were recorded as 4.63 ± 0.93 and 0.954 ± 0.318 ng/ml in pregnant and nonpregnant buffaloes, respectively. Pregnancy diagnosis in Group III (Punyakoti test) was undertaken using seed germination inhibition test at day 26 post insemination and the buffaloes diagnosed pregnant showed significant inhibition of seed germination and shoot length than the nonpregnant buffaloes with a mean germination inhibition percentage (%) and shoot length (cm) of 70.66 ± 1.63 ; 3.33 ± 0.074 and 45.99 ± 1.84 ; 5.44 ± 0.17 in pregnant and nonpregnant buffaloes, respectively. Buffaloes in Group IV (Transrectal ultrasonography) were analyzed for early pregnancy using ultrasound at day 20, 25 and 30 post insemination. Pregnant buffaloes showed anechoic embryonic vesicle within the echogenic uterus on day 20, expansion of embryonic vesicle and embryo proper in some buffaloes on day 25 and clear visualization of anechoic embryonic vesicle and echogenic embryo proper in all pregnant buffaloes on day 30 post insemination. Confirmation of pregnancy in all the buffaloes that were selected in the four test groups was done by rectal examination at day 45-60 post insemination. The overall diagnostic accuracy of Group I, II, III and IV was 93.75, 85.00, 75.00 and 100 per cent on days 21, 21, 26 and 30 post insemination. The study concluded that Heat-aid kit and Transrectal ultrasonography was 100 per cent accurate in diagnosing nonpregnancy. Among all the diagnostic techniques in the present study, transrectal ultrasonography was more accurate in diagnosing pregnancy and nonpregnancy from day 25 post insemination.
Open Access
EFFECT OF VARIOUS GROWTH FACTORS AND HORMONES ON IN VITRO CULTURE OF GOAT PREANTRAL FOLLICLES
(SRI VENKATESWARA VETERINARY UNIVERSITY , TIRUPATI – 517502. (A.P.) INDIA, 2013-02) TEJA, ALLU; SADA SIVA RAO, K (Major); ARUNA KUMARI, G; RAGHAVENDER, K. B. P
Open Access
COMPARATIVE EVALUATION OF MODE OF ADMINISTRATION (SINGLE AND MULTIPLE DOSES) OF FSH ON SUPEROVULATORY RESPONSE IN SAHIWAL COWS
(SRI VENKATESWARA VETERINARY UNIVERSITY , TIRUPATI – 517502. (A.P.) INDIA, 2013-08) VENKATESWARA REDDY, Ch; Veerabramhaiah, K (Major); Mouli Krishna, K; Veena, P
ABSTRACT : Superovulaory response with different mode of administration of FSHp was studied in Sahiwal cows based on estrus response, number of ovulations, embryos recovery and anovulated follicles including endocrine response. All the selected donor cows were synchronized with double PG prior to superovulation and on 3rd day of superovulation with single PG. The difference in the interval between PG treatment and onset of estrus and duration of estrus between the cows synchronized prior to and after superovulation with 250 mg of FSHp in multiple doses (group I) and single dose (group II) was significant (P<0.05). Higher percentage of cows showed intense estrus in group I cows and intermediate estrus in group II cows. Superovulation of group I donor cows resulted in development of 8.50 ± 0.56 and 5.67 ± 0.56 mean number of follicles and mean number of ovulations, respectively and the same in group II donor cows were 5.17 ± 0.17 and 3.33 ± 0.21, respectively. The difference in the total follicles and total ovulations were significantly (P<0.05) higher in group Icows than in group II 12 cows. The overall total mean number of anovulations were significantly higher in group I (2.83 ± 0.17) compared to group II (1.83 ± 0.31). The overall mean number of embryos, transferable embryos and unfertilized ova recovered from group I donor cows were 3.17 ± 0.65, 2.33 ± 0.42 and 0.83 ± 0.31, respectively per collection with an overall mean recovery percentage of 55.88 ± 7.68. The corresponding values in group II donor cows were 1.67 ± 0.21, 1.00 ± 0.26 and 0.67 ± 0.21, per collection with an overall mean recovery percentage of 50.00 ± 6.09. The total number of embryos and transferable embryos recovered after superovulation were insignificantly (P>0.05) higher in group I cows than in group II cows. Embryos collected were cryopreserved by OPS vitrification method. In Group I and II, the overall mean progesterone concentrations recorded on different days of synchronization and superovulation i.e., at 2nd PGF2α, synchronized estrus, initiation of superovulation, PGF2α on 3rd day of superovulation, super estrus and embryo collection were 5.08 ± 0.83, 2.24 ± 0.22,5.24 ± 0.53, 5.12 ± 0.53, 1.93 ± 0.60 and 6.53 ± 0.41 ng/ml, respectively and 3.98 ± 0.83, 2.47 ± 0.22,5.39 ± 0.53, 4.31 ± 0.53, 2.49 ± 0.51 and 4.80 ± 0.56 ng/ml, respectively. The overall mean estrogen concentrations on same days were 15.05 ± 0.55, 26.30 ± 1.20, 17.46 ± 0.54, 32.16 ± 2.41, 39.04 ± 1.06 and 17.72 ± 0.45 pg/ml, respectively in group I and 20.20 ± 0.99, 24.14 ± 0.86, 21.76 ± 0.74, 32.41 ± 2.08, 38.22 ± 0.86 and 20.05 ± 1.03 pg/ml, respectively in group II. The concentrations of progesterone and estrogen at the time of embryo collection were significantly (P<0.05) different between Group I and II. But the difference between the groups at 2nd PGF2α administration and synchronized estrus and after administration of PGF2α, at the time initiation of superovulation, PGF2α administration, super estrus and embryo collection was not significant (P>0.05). There was a positive and significant (P<0.05) correlation between concentrations of progesterone at synchronized estrus and super estrus in group I while a negative and significant (P<0.05) correlation between 2nd PG and anovlations;initiation of superovulation and embryo collection and superovulation PG and embryo collection in group II. The concentration of estrogen at 2nd PG with recovered embryos and transferable embryos and at initiation of superovulation with anovulations was positively and significantly (P<0.05) correlated in Group I and at 2nd PG with recovered embryos and 13 transferable embryos and at initiation of superovulation with transferable embryos was negatively and significantly (P<0.05) correlated.
Open Access
PATHOMORPHOLOGY OF UTERINE NEOPLASIA IN INFERTILE CROSSBRED COWS
(SRI VENKATESWARA VETERINARY UNIVERSITY , TIRUPATI – 517502. (A.P.) INDIA, 2013-03) SUNNY PRAVEEN, K; Mouli Krishna, K (Major); Veerabramaiah, K; Srilatha, Ch
ABSTRACT : The objective of the present study was to ascertain the prevalence and cytological, histopathological description of uterine neoplasia in infertile crossbred cows. To fulfill the objective, cytopathology, histopathology and AgNOR were taken up. In the present study clinical and gross observations in slaughter house crossbred genitalia were uterine wall thickening (61.53%), purulent discharge (21.15%), slimy discharge (9.61%), whitish foci over endometrium (21.15%), endometrial congestion (11.53%), petechiae over caruncles (7.69%) and atrophied caruncles (flattened endometrium) (5.76%). Furthermore clinical observations in infertile crossbred cows noticed were thickened uterus (73.68%), extended estrus duration (68.42%), irregular estrus cycle (78.94%), and copious discharge (52.63%). Cytological studies revealed abnormal cellular pattern suspicious of neoplasia in 82.69% in slaughter house genitalia and 42.10% in infertile crossbred cows. Whereas, 17.30% slaughter house genitalia and 57.89% infertile crossbred cows presented normal epithelial cellular pattern. Based on histopathological studies 73.07% neoplasia and 21.15% dysplasia in slaughter house genitalia, whereas 31.57% neoplasia and 5.26% eosinophilia in infertile crossbred cows. However chronic endometritis was found in 5.76 and 5.26% in slaughter house genitalia and infertile crossbred cows, respectively. The neoplasms recorded in slaughter house crossbred genitalia were adenocarcinoma (65.78%), followed by lymphosarcoma (7.90%), haemangiosarcoma and haemangiosarcoma with adenocarcinoma both accounted to 5.26% each. In adenocarcinoma, histiocytic adenocarcinoma, scirrhous adenocarcinoma, clear cell adenocarcinoma, and papillary adenocarcinoma were accounted to 20 (5), 12 (3), 12 (3) and 4% (1), respectively. Other neoplasms reported were histiocytoma, fibrosarcoma, leiomyosarcoma, myxosarcoma, leiomyosarcoma with haemangiopericytoma, leiomyosarcoma with adenocarcinoma and lymphosarcoma with adenocarcinoma each accounted to 2.63%. Whereas in infertile crossbred cows the highest prevalence of the neoplasm in uterus was adenocarcinoma, haemangiosarcoma accounted to 50% each. In adenocarcinoma, clear cell adenocarcinoma accounted to 33.33%. The mean AgNOR count varied from 5.2 to 8.7 with a mean of 6.45 ± 0.06 per cell in neoplasia. Whereas for dysplasia and chronic endometritis AgNOR count varied from 4.0 to 4.3 and 3.1 to 3.2 with a mean of 4.17 ± 0.09 and 3.16 ± 0.10 per cell, respectively. The AgNOR counts significantly differed between groups (P ≤ 0.05). Finally it was concluded that uterine neoplasia could be one of the contributing factors to infertility and special focus may be given to cytopathology so as to initially screen the chronic infertile cows for neoplasia.
Open Access
EVALUATION OF OXIDATIVE STRESS IN TRANSITION COWS AND POST PARTUM ENDOMETRIAL CYTOLOGY
(SRI VENKATESWARA VETERINARY UNIVERSITY , TIRUPATI – 517502. (A.P.) INDIA, 2013-03) RAVI BABU, G; Mouli Krishna, K; Veerabramaiah, K; Padmaja, K
ABSTRACT : The transition period is physiologically the most important, highly sensitive and venerable in the reproductive life of dairy cows. The physiological and metabolic changes occurring in peripartum period imposes oxidative stress and directs the subsequent reproductive events in dairy cows. The objective of the study was to evaluate oxidative stress, antioxidant status and endometrial cytology for predicting the uterine involution in cows. A total of 14 multiparous crossbred cows maintained in a commercial farm were assigned to group I (8 Healthy) & II (6 Infected). Blood samples were collected periodically on day -10, 0, 10, 25 and CUI. Spectrophoto-metric procedures were adopted to determine concentrations of MDA, Vit.E, GSH, glucose, total proteins and SOD and Catalase activity, in addition to endometrial cytological smears on day 25 and CUI. Neutrophils counts were decreasing after day 25 and in infected group II the counts were significantly higher. Values of MDA, Vit.E, GSH and Catalase activity were significantly different (p≤0.05) between collections in group I and II while, glucose and total proteins remained within physiological ranges. Progesterone concentration significantly different (p≤0.05) between collections in group I and II. The cows in group II had high lipid peroxidese and low antioxidant status during early part of the experiment and was affected with uterine infections. It was concluded that there were significant differences between two groups of cows investigated. The high MDA profiles recorded on day 0 and low antioxidant status observed in group II animals might have compromised host`s immune defense and suffered them from uterine infections. Further, the presence of neutrophils significantly in high percentages in uterine cytological smears obtained at CUI (after morphological completion of uterine involution) indicates the persistence of infection after declaring completion of involution. From the present study, it was suggested that the routine rectal examination of the tract is insufficient to conclude completion of involution. It was felt that in routine practice the endometrial cytology may be adopted in clinical diagnosis of uterine involution.

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