Tamil Nadu Veterinary and Animal Sciences University, Chennai
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Browsing Tamil Nadu Veterinary and Animal Sciences University, Chennai by Subject "Animal Biotechnology"
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ThesisItem Open Access 16S rRNA Typing for Identification of Gut Microbes in Termites(TANUVAS, Chennai, 2011) Kavitha, D.; Vijayarani, K.; Karunakaran, R.; TANUVASThesisItem Restricted A PROTEOMIC APPROACH TO AUGMENT QUALITY OF CHICKEN MEAT THROUGH DIETARY NATURAL ANTIOXIDANTS(2021) DIVYA MANJARI K; TANUVAS; PARTHIBAN M; KARUNAKARAN R; APPA RAO V; SENTHILKUMAR TMAProbiotics were reported to improve body weight gain and feed efficiency and reduced mortality in broiler chicken. In continuation of using probiotics as performance enhancers in broilers prebiotics are also supplemented to enhance the activity of probiotics and also aid in scavenging free radicals that are responsible for creating stress in the mitochondria. Here in this study chicory is a natural antioxidant that is used as prebiotic as feed supplement. In this study a total of 21 probiotic organisms belonging to seven different species were isolated from chicken gut. These twenty one probiotic organisms were characterized by Gram staining, Biochemical methods using 21 sugars, pH tolerance and bile salt tolerance test, enzyme analysis using 19 different enzymes. These probiotic organisms were identified using molecular studies using PCR amplification of 16S -23S spacer region and sequencing analysis. Based on the sequencing data, seven different species were found to exist namely Enterococcus faecalis, Enterococcus faecium, Enterococcus hirae, Enterococcus durans, Lactobacillus salivarius. Bacillus subtillis and Pediococcus acidilactici. Based on extracellular and in vitro assays a scoring system was evolved to evaluate the potential probiotic bacteria with antioxidant property. These probiotic bacteria along with prebiotic chicory was supplemented in broilers and the performance was studied and observed that the live weight was increased by 200 grams in the synbiotic treated groups. The pH, meat tenderness and total protein were also improved in synbiotic treated groups. Also the proteome of the breast muscle was studied for differentially expressed proteins which relate to the enhancement of broiler performance in the experimental trial.ThesisItem Open Access Amplification and Cloning of Manganese Peroxidase Gene from Phanerochaete chrysosporium(TANUVAS, Chennai, 2012) Anjpriya, R.; Kumanan, K.; Vijayarani, K.; TANUVASThesisItem Open Access ANALYSIS OF BOVINE SPERM ASSOCIATED MEMBRANE PROTEINS BY TWO DIMENSIONAL GEL ELECTROPHORESIS(TANUVAS, Chennai, 2017) Muralidharan, T.; Palanisammi, A.; TANUVAS; Tirumurugaan, K.G.; Sivaselvam, S.N.This study was carried out in cattle sperm in the aspect of proteomics to find out the differences between the X- & Y- spermatozoa as far as the membrane proteins are concerned. It is a known fact that there are certain proteins present in the plasma membrane of the spermatozoa which are sex-specific and are called as sex associated or sperm associated membrane (SAM) proteins.ThesisItem Open Access ASSESSMENT OF SPERM FERTILITY BIOMARKER USING FLOW CYTOMETRY AND ITS CORRELATION WITH IN VITRO PRODUCTION OF BOVINE EMBRYOS(TANUVAS, Chennai, 2017) Rajaram, V.; Palanisammi, A.; TANUVAS; Kumanan, K.; Sivaselvam, S.N.Osteopontin is a 55 kDa seminal plasma protein which is found predominantly in highly fertile bulls. The present study involves exploring the utility of osteopontin as a fertility biomarker and also to study its influence on in vitro fertilization. In this study, semen from six Jersey crossbred bulls were collected and sperm parameters like motility, viability, abnormality, plasma membrane integrity, acrosomal integrity, sperm chromatin structure assay and oxidative stress were studied by flow cytometry.ThesisItem Restricted BACILLUS SPP AS A NOVEL BIOCONTROL AGENT AGAINST COCCIDIOSIS IN BROILERS(2018) Aadithya, T; Meignanalakshmi, S; Parthiban, M; Raman, M; TANUVASIn the present study, PHB a biopolymer produced by Bacillus spp was evaluated against coccidiosis. PHB production by Bacillus spp was screened by Sudan Black staining and confirmed by Nile Red staining. The produced PHB was characterized by FTIR, NMR and XRD analysis. The characterized PHB was further used to evaluate its effect against sporulation inhibition and cell wall integrity of E.tenella oocyst. PHB at different doses (10, 20, 50 and 100mg) were used for sporulation inhibition studies. Potassium dichromate (2.5%) and oocyst suspension were used as positive and negative control. The sporulated and unsporulated oocyst were counted at 0, 24 and 48 hrs interval and sporulation inhibition percentage was calculated. PHB at the dose rate of 100mg (62.40%) has highest effect on inhibiting the sporulation of E.tenella oocysts when compared to other doses of PHB 50mg (53.33%), 20mg (45.42%), 10mg (46.20%) and positive control group (8.60%) at 24 hours. At 48 hrs, PHB at the dose rate of 100mg (31.70%) showed highest inhibition effect when compared to other doses of PHB 50mg (25.65%), 20mg (19%), 10mg (13.90%) and positive control group (8.60%). At the dose rate of 100mg of PHB, breakage was noticed at the outer proteinaceous wall of E.tenella oocyst.ThesisItem Open Access Bio-Compatability Of Biological Implants In The Treatment Of Induced Thermal Injuries In Dogs(1998) Seshachalam R; TANUVASThesisItem Restricted BIOHYDROGEN PRODUCTION FROM RUMINAL CONTENTS(2021) Merli Maman; TANUVAS; Meignanalakshmi S; Ghadevaru Sarathchandra; Vijayarani K; Meenakshi SundaramHydrogen gas is a promising alternative for fossil fuel at the current time due to the advantages that is environment friendly, it can be derived from renewable sources and it has high energy efficiency. In the present study, hydrogen gas was producedin a biological way by using rumen fluid of cattle collected from slaughter house by dark fermentation of rumen fluid and Microbial Electrolysis Cell (MEC) process. Integration of both dark fermentation and MEC was also carried out. Dark fermentation of rumen fluid was carried out in 100 ml air tight serum bottles using rumen fluid as the substrate and microorganisms present in the rumen fluid as inoculum and the production of biohydrogen was confirmed by gas chromatography with Thermal conductivity detector (GC-TCD). The dark fermentation parameters such as pH, substrates and micronutrient concentrations were optimized. The pH 4, 5, 6, 7 and 8 were used for dark fermentation of rumen fluid and it was found that pH 6 produced higher biohydrogen when compared to other pH. Substrates such as 1% rice bran, 1% powdered potato peel and 1% powdered tapioca peel were used as added substrates to rumen fluid for dark fermentation and it was found that 1% rice bran produced higher biohydrogen. The micronutrients used were O.25M, 0.5M, 0.75M and IM concentrations of Ferrous sulphate. Magnesium sulpahte and Potassium sulphate. It was found that in all the three mucronutrients, 0,5M concentration produced higher biohydrogen. Rumen fluid collected from slaughter house was used for isolation of biohydrogen producing microorganisms and four different biohydrogen producing bacteria were isolated, characterized and the production of biohydrogen was also confirmed using the individual bacteria in dark fermentation. The rumen fluid after dark fermentation was also given for metagenomic analysis to find out the phylum, genus and species abundance in the rumen fluid. The Microbial electrolysis cell (MEC) was designed and made for the biohydrogen production. The parameters such as pH, salt-bridge, catholyte, electrodes and substrates were optimized for the production of biohydrogen. Among the pH 4, 5, 6, 7 and 8, the highest amount of biohydrogen production was obtained in pH 6. 0.8% and 1% of agarose and agar-agar were used as salt-bridge in the MEC and the highest biohydrogen production was obtained in MEC with 0.8% agarose. The electrodes used were carbons rods and graphite plates where the MEC with graphite plates as the electrode showed higher biohydrogen production. Sodium chloride in different concentrations such as 0.25M, 0.5M, 0.75M and IM were used and it was found that 0.5M Sodium chloride produced highest amount of biohydrogen in MEC. The substrates added to the rumen fluid in the anode chamber were 1% rice bran, 1% powdered potato peel and 1% powdered tapioca peel and the highest amount of biohydrogen production was obtained in the MEC with 1% rice bran.The integration of dark fermentation and MEC was carried out for an enhanced amount of biohydrogen production. The total amount of biohydrogen produced was calculated from the individual amount of biohydrogen produced by dark fermentation and MEC. The quantity of biohydrogenproduced were statistically analysed and the obtained p value is less than 0.005 (p<0.005) which shows that the quantity of biohydrogen produced with different pH and substrates were statistically significant.ThesisItem Open Access Biotechnological Methods in Peste Des Petits Ruminants Diagnosis(TANUVAS, 2000) Brindha, K; TANUVAS; Raj, G. Dhinakar; Nachimuthu, K; Nainar, A. MahalingaThesisItem Open Access Characterisation of Indigenous Sheep Breeds by Random Amplified Polymorphic DNA (RAPD)(TANUVAS, 1998) Nagarajan, G; TANUVAS; Nainar, A. Mahalinga; Nachimuthu, K; Raman, K.S.ThesisItem Open Access Characterization of Buffalo Embryonic Cells Using A Specific Marker(TANUVAS, 2010) Vedamanickam, Stalin, A; TANUVAS; Palanisamy, A; Kumanan, K; Ramaswamy, VThesisItem Open Access CHÅRACTERIZATION OF EMBRYONIC STEM CELL LIKE FROM PRE-HVIPLANTATION GOAT (CAPRA HIRCUS) EMBRYOS(TANUVAS, Chennai, 2015) Ramkumaar, R.K.; TANUVAS; Palanisammi, A.; Vijayarani, K.; Asokan, S.A.This study was carried out in goat (Capra hircus) pre-implantation embryos from which the stem cell like cells have been isolated and been characterized. Establishment of stable immortal ES cell lines using embryo as a source of isolation in domesticated farm animals, in particular for Goat, which are closer to human than mouse has not been reported. This information could contribute to the improvement of agricultural biotechnology, genetic modifications, developmental biology and regenerative medicine in humans, biotechnology and agriculture. Therefore, the discovery of effective protocols to derive and maintain ES cells and the induction of somatic cells from ES cells in goat is of importance.ThesisItem Open Access Characterization Of Fusion Gene Of Newcastle Disease Viral Isolates(Animal Biotechnology ; Chennai, 1997) Ramesh Babu Ng; TANUVASThesisItem Open Access Characterization of Leptospiral Serovars by Monoclonal Antibodies and RAPD Methods(Tamil Nadu Veterinary and Animal Sciences University, 2002) Senthilkumar, T.M.A.; TANUVAS; Ramadass, P.ThesisItem Open Access Characterization Of Leptospiral Serovars By Monoclonal Antibodies And Rapd Methods(Tamil Nadu Veterinary and Animal Sciences University, 2002) Senthil Kumar, T.M.A; TANUVAS; Ramadas, PThesisItem Open Access Characterization Of New Castle Disease Virus Isolates Form Desi Chicken(1996) Raghavan V S; TANUVASThesisItem Open Access Characterization of Newcastle Disease Virus Isolates from Desi Chicken(TANUVAS, 1996) Raghavan, V.S.; TANUVAS; Kumanan, K; Ramadass, P; Manohar, B. MuraliThesisItem Open Access Characterization of Secretome from Amniotic Membrane Derived Cells(TANUVAS, Chennai, 2014) Rajaram, Sasane Manoj; TANUVAS; Mangalagowri, A.; Ramani, C.; Senthilkumar, T.M.A.