Browsing by Author "Singh, Vijay"
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ThesisItem Open Access Development of inclined plate metering device for manually operated onion bulb planter(JNKVV, 2015) Singh, Vijay; Nilatkar, D.K.ABSRTRACT India is the second largest producer of onion in the world next after China. Mechanization of farming is increasing day by day to produce yield of crop for gaining the profit. Mechanizination not only increases the production and productivity, but also reduces the labour cost and drudgery. As a result it produced more within the shortest possible period of time by using minimum manpower and improved cultural practices. This present study is related to the development of an inclined plate metering device for planting onion bulb for the seed purpose and this metering device is applicable to meter a small onion bulb usually less than an inch in diameter which is used for propagation. The main concept of design and development of onion bulb planter is metering device which is simple in their structure and easy to manufacture. In this study,three differentinclination angles of 500, 600 and 700evaluated for elevating error, cell fill and bulb damage at different peripheral speed and also evaluated the seed uniformity was describe by using the actual planting distance, mean plating distance, planting error and the quality of feed index at different travel speed under the laboratory and the precision in addition to the means and standard deviations of the sample method. The result indicated that the elevating error was minimum at the inclination angle of 500 is 2.27 % at the peripheral speed of 7.6 m/min and maximum 10.82 % at the peripheral speed of 45.59 as compare to 600 and 700i.e. 2.28 to 11.63 and 4.95 to 13.77% at different peripheral speeds (7.6 to 45.59 m/min) it also indicate the peripheral speed increased the elevating error is also increase.ThesisItem Open Access Effect Of Addition Of Glutathione On Tyrosine Phosphorlation And Apoptosis Like Changes In Cryopreserved Hariana Bull Semen(U.P. Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, (DUVASU), Mathura - 281001, 2016-07-27) Shah, Nadeem; Singh, VijayThe present study evaluated the beneficial effects of incorporation of Glutathione as an additive in Tris egg yolk based extender in Hariana bull semen opted for ultralow freezing. The study valuated physical seminal attributes (motility, livability and membrane integrity), process of ryocapacitation, immunoblotting for identification of tyrosine phosphorylated proteins, immunolocalization of osine phosphoproteins in spermatozoa, and apoptosis like changes in terms of mitochondrial transmembrane potential and DNA integrity during after equilibration and thawing. Ten ejaculates from four Hariana bulls were divided into three aliquots: One aliquot diluted with egg yolk tris citrate (EYTC) extender (Control), second aliquot was diluted with EYTC but supplemented with 0.5Mm Glutathione (T1) and the third aliquot was diluted with EYTC and supplemented with 1.0Mm Glutathione (T2) and were cryopreserved. Semen evaluation at equilibration and post-thaw showed supplementation of Glutathione (0.5Mm) to EYTC extender significantly (P < 0.05) increased motility, viability and membrane integrity of spermatozoa. The degree of cryocapacitation was significantly (P < 0.05) decreased in Glutathione supplemented group. Immunoblot revealed six proteins which were tyrosine phosphorylated and protein of 30kDa (p30) showed differential variation in intensity in the three samples. There was significant reduction in band intensity of 30kDa in T1 as compared to control and T2. It was also found that tyrosine phosphorylated proteins were differently located during different stages of semen preservation. Addition of GSH significantly decreased percentage of spermatozoa showing fragmented DNA after thawing as compared to control. Along with this, GSH supplementation significantly increased the percentage of spermatozoa with high transmembrane mitochondrial potential. The result of the present study clearly demonstrated beneficial effects of Glutathione supplementation on post thaw cryocapacitation and apoptosis like changes in spermatozoa and it can be suitably incorporated for long term preservation of spermatozoa.ThesisItem Open Access Effect of pfertility and potassium levels on yield and nutrient composition of wheat under irrigated conditions(jnkvv, jabalpur, 2001) Patil, Ajay; Singh, VijayThesisItem Open Access Effect of Pre-Harvest Sprays of Plant Growth regulators and Chemicals on shelf Life of Mango(jnkvv, jabalpur, 2004) Singh, Vijay; Patel, R. M.ThesisItem Open Access Endocrinological and biochemical Studies During Prepuberal and Puberal Periods in Female Goats (Capra Hircus)(College of Veterinary Sciences Chaudhary Charan Singh Haryana Agricultural University Hisar, 1992) Singh, Vijay; Bugalia, N. SThesisItem Open Access Estimation of crossbreeding parameters for production traits in complete diallel cross involving two native and one exotic chicken breeds(IVRI, Izatnagar, 2016) Singh, Vijay; Raj NarayanThesisItem Open Access Farmers Knowledge and Adoption of Integrated Pest Management Practices in Sugarcane cultiation in Haryana(College of Agriculture Chaudhary Charan Singh Haryana Agricultural University Hisar, 1981) Singh, Vijay; laharia, S. N.ThesisItem Open Access Functional characterisation of TRPV1 channel in bull spermatozoa(U.P. Pandit Deen Dayal Upadhyaya Pashu-Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, (DUVASU), Mathura – 281001, 2018-06-23) Kumar, Akshay; Singh, VijayCalcium influx in flagellated cell like spermatozoa plays critical role in regulation of sperm motility, capacitation, hyperactive motility and acrosome reaction. Calcium influx is primarily mediated by Catsper channels and transient receptor potential (TRP) channels. TRP Vanilloid 1 is considered as most versatile thermosensitive, pH sensitive and chemosensitive ion channel found in spermatozoa. Little information is available with contrasting reports regarding the role of TRPV1 in regulation of sperm function. Molecular and functional characterisation of TRPV1 was carried out in spermatozoa of Hariana bulls. Sixty four ejaculates were collected from four bulls and were used for series of experiments. Immunoblotting and immunocytochemistry were employed for the molecular characterisation of TRPV1. Immunoblotting identified a single band of 104 kDa corresponding to TRPV1 in Hariana bull spermatozoa. Positive immune-reactivity was seen in acrosomal, preacrosomal, post acrosomal and flagellar regions corresponding to TRPV1. Functional study was carried out using TRPV1 blocker namely Capsazepine (Cp) @ 10µM and one activator was used namely Anandamide (AEA) @ 0.3µM. In the study, three groups were used namely, control (100 µL of sperm dilution medium (SDM) containing 1×106 cells), vehicle (3µL) and drug (Cp, AEA and their combinations). Different time of incubations was used depending on the experiments. Blocking of TRPV1 resulted in significant (P<0.05) reduction in progressive sperm motility as compared to the control; and with activation using AEA, PSM was decreased significantly (P< 0.05) till 1h and after that PSM was sustained as compared to control. However, both during blocking and activation of TRPV1, per cent spermatozoa showing hyperactive motility was increased (20-30%) (P< 0.05). Evaluation of Cp and AEA treated spermatozoa stained with CTC revealed significant (P< 0.05) increase in B-pattern of spermatozoa indicating induction of capacitation. Spermatozoa treated with different pH gradients showed significant (P< 0.05) reduction in motility as compared to control both with and without drugs modulating TRPV1. Functions of TRPV1 were found to be mediated through cAMP and PKA pathway in the induction of hypermotility in sperm cells as evident from inhibition of sAC and PKA. Both L- and T- type of calcium channels were found to be associated with TRPV1 function as evident from their respective blocking and its effect on PSM. Blocking as well as activation of TRPV1 showed significant (P< 0.05) reduction in sperm livability, per cent spermatozoa having intact membrane, per cent spermatozoa having intact acrosome, per cent spermatozoa showing high mitochondrial transmembrane potential indicating the involvement of TRPV1 in the process of regulation of sperm functional dynamics. From the study, it was concluded that TRPV1 channels are found in bull spermatozoa and are pH dependent. These channels mediate number of sperm functions like hyper motility, capacitation and acrosome reaction through complex interacting pathways through calcium and pH dependent mechanisms. Further studies are required to find out the possible relationship between TRPV1 channels and other channels in regulating spermatozoa function and possible mechanisms associated with TRPV1 activation as well as its role in sperm function regulationThesisItem Open Access Functional Characterisation of Voltage -Gated Potassium Channels in Bull Spermatozoa(U.P.Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan Mathura-281001(UP, 2017-07-07) Gupta, Rishi kumar; Singh, VijayIn the current study , molecular and functional characterisation ok Kv 1.I was carried out in spermatozoa of experiments .Sixty four ejaculates were collected from hariana bulls and were molecular characterisation of K.v.1.Immunoblotting identified a single band of 56 k da corresponding to Kvl 1 in Hriana bull spermatozoa.Positive immunoreactivity-------------------------------------------functionThesisItem Open Access Genetic analyses of growth and immunocompetence in different breeds of ducks(IVRI, Izzat Nagar, 2013) Singh, Vijay; Mishra, S. K.ThesisItem Open Access Impact of jalagam prabandh pariyojana on dairy farmers of Tehri district (U.P)(NDRI, KARNAL, 1993) Singh, Vijay; Tyagi, K CThesisItem Open Access Investigations on therapeutic aspects of erythrocytic oxidative stress and hypophosphatemia in buffaloes (Bubalus bubalis)(College of Veterinary Sciences Chaudhary Charan Singh Haryana Agricultural University Hisar, 2003) Singh, Vijay; Jain, V. KThesisItem Open Access Phytophagous mites and their natural enemies in different horticultural ecosystems of Himachal Pradesh(YSPU, 2015) Singh, Vijay; Chauhan, UshaOf the seventy nine species recorded in the present study, twenty one species were of phytophagous mites and fifty eight were natural enemies. Phytophagous mites were Tetranychus urticae Koch, T. ludeni Zacher, T. sp. nr. ludeni, T. hypogeae Gupta, T. neocaledonicus Andre, T. udaipurensis Gupta & Gupta, T. sayedi Baker & Pritchard, T. macfarlanei Baker & Pritchard, T. sp. nr. macfarlanei, Panonychus ulmi (Koch), P. citri (McGregor), Eotetranychus frosti McGregor, Eutetranychus orientalis (Klein), Bryobia praetiosa Pritchard & Baker, Porcupinychus abutiloni Anwarullah, Brevipalpus essigi Baker, B. cucurbitae Mohansundaram, B. euphorbiae Mohansundaram, Aculus malus Zaher & Abou Awad, Aceria litchi (Keifer) and Tarsonemus sp. Of fifty eight natural enemies, forty six were predatory mites and twelve species were of predatory arthropods. Predarory mites were Amblyseius aerialis (Muma), A. largoensis (Muma), A. cucurbitae Rather, A. herbicolus (Chant), A. multidentatus (Swirski & Shechter), A. guajavae Gupta, Euseius prasadi (Chant & McMurty), E. eucalypti Ghai & Menon, E. sp. nr. ahaioensis, E. alstoniae Gupta, E. finlandicus (Oudemans), E. neococcinae Gupta, E. sp. nr. coccineae, E. delhiensis (Narayanan & Kaur), E. chitradurgae Gupta, N. paspalivorus (DeLeon), N. longispinosus (Evans), N. sp. nr. neoghanii, Typhlodromus (Anthoseius) hadii Chaudhri, T. (A.) mori Gupta, T. (A.) pruni Gupta, T. (A.) sp. nr. channabasavannai, T. (A.) himalayensis Gupta, T. (A.) darjeelingensis Gupta, T. (A.) sp. nr. darjeelingensis, Phytoseius roseus Gupta, P. maldahensis Gupta, P. sp. nr. maldahensis, P. intermedius Evans & Mcfarlanne, P. crinitus Swirski & Shechter, P. corniger Wainstein, P. kapuri Gupta, Agistemus fleschneri Summers, A. terminalis (Quayle), A. obscura Gupta, A. sp. nr. obscura, A. sp. nr. macrommatus, A. sp. nr. heterophylla, Tydeius cumini Gupta, Pronematus fleschneri Baker, Acarus gracilis Margaret, Tyrophagus putrescentiae (Schrank), Anystus baccarum (Linnaeus), Walzia darjeelingensis Gupta, Lasioseius sp., Leptus sp. Eleven species were new record. T. urticae, T. (A.) mori and T. (A.) himalayensis were relatively abundant on fruits, T. urticae, Euseius prasadi and E. delhiensis on ornamental and T. urticae, A. largoensis and E. finlandicus on vegetable crops. Maximum number of prey and predatory mites were recorded on fruits (48) followed by vegetables (25) and ornamentals (17). Maximum numbers of natural enemies were also recorded on fruits (36) as compare to vegetables (21) and ornamentals (17). The most dominant species on apple were T. urticae, P. ulmi, T. (A.) himalayensis and T. (A.) mori, whereas, on rose were T. urticae, Feltiella sp. and E. prasadi, on carnation were, T. urticae, T. ludeni and S. sexmaculatus, on cucumber were T. macfarlanei and A. largoensis and on tomato were T. ludeni and N. sp. nr. neoghanii. T. (A.) hadii and Oligota sp. were the new record on apple and rose in the state.ThesisItem Open Access Relative performance of important cultutivars of coriander (Coriandrum sativum L.) As Affected by Row spacing and nitrogen fertilization(MPUAT, Udaipur, 1993) Yadav, Surendranath; Singh, VijayThesisItem Open Access Relative performance of important cultutivars of coriander (Coriandrum sativum L.) As Affected by Row spacing and nitrogen fertilization(MPUT, UDAIPUR, 1993) Yadav, Surendra nath; Singh, VijayThesisItem Open Access Residual effect of fertility levels and sulphur soruces applied to black gram on yield and quality of wheat(JNKVV, Jabalpur, 1999) Yadav, Padam Singh; Singh, VijayThesisItem Open Access Studies on assessment of capitation like changes in the cryopreserved sperms in Hariana Bull(College of Veterinary Science and Animal Husbandry, U.P.Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalay Evam Go-Anusandhan Sansthan, Mathura 281001, 2015-07-14) Verma, Meena; Singh, VijayThesisItem Open Access Studies on effect of additives on cryopreservation of Hariana bulls semen(College of Veterinary Science and Animal Husbandry, U.P.Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalay Evam Go-Anusandhan Sansthan, Mathura 281001, 2014-01-06) Pal, Ajendar Singh; Singh, Vijay