Browsing by Author "Singh, Amit Kumar"
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ThesisItem Open Access Biometrical Analysis in Vegetable Pea (Pisum sativum L.)(College of Horticulture, Bharsar Campus, V.C.S.G. Uttarakhand University of Horticulture and Forestry, 2015-07) Singh, Amit Kumar; Nautiyal, Dr. B.P.Pea (Pisum sativumL.) genotypes were evaluated to study variability, correlation, path analysis and genetic divergence. The experiment was laid out in a RBD with three replications. Thirty two genotypes including two check cultivars were evaluated for different horticultural traits. Analysis of variance showed highly significant differences among all the characters under study. Fifteen genotypes viz Palam Triloki, Arka Karthika. Pant Pea-96, Kashi Udai, DPP-3, Pant Pca-86, Kashi Shakti, Pant Pea-74, Palam Priya,PSM- 5, Arka Ajii, Pant Pea-14, VL-12 and Arkcl were superior over other entries and over standard checks for yield, quality and other important horticultural traits. High heritability coupled with high genetic advance as percent of mean were observed for number of rode per plant, plant height, pod yield per plant and pod yield per plot indicating presence of additive gene effects suggesting more scope of selection for these traits. The phenotypic and genoiypic correlation coefficients among different characters showed that yield has positive association with number of branch per plant, number of pod per plant, number of cluster per plant. The path coefficient analysis revealed that, number of pods per plant followed by number of seeds per pod, number of branch per plant, internode length and dry matter content have maximum positive direct effect on pod yield per plant. While, number of cluster per plant, harvest index, shelling percentage, plant height, pod length, days to 50 % germination, number of node per plant and days to 50 % flowering were observed to have negative direct effect on pod yield per plant. These findings indicate that selection may be made on the basis of above mentioned characters for improving yielding ability in the pea. These findings showed that selection should be made on the basis of above mentioned characters for improved yield in pea. Studies on 32 genotypes of pea were grouped into five clusters and maximum number of genotypes was arranged in cluster IV which contained 10 genotypes. All the five clusters showed genetic diversity and the genetic diversity was independent of geographic diversity. Maximum average intracluster distance was found in V and minimum in III. Highest inter cluster distance was recorded between cluster V and II and lowest was observed between cluster IV and III. Indicating that hybridization between genotypes from cluster V and III can be utilized for getting the superior recombinants / transgressive segregants in segregating generations in pea.ThesisItem Open Access Comparative study on contract and noncontract broiler farming systems in Eastern Plain zone of Uttar Pradesh(IVRI, Izatnagar, 2015) Singh, Amit Kumar; Sagar, M.P.ThesisItem Open Access Development of transgenic groundnut with Citrate synthase gene through Agrobacterium-mediated transformation for improving phosphorous use efficiency(University of Agricultural Science, Dharwad, 2002) Singh, Amit Kumar; Gowda, T K SABSTRACT NOT AVAILABLEThesisItem Open Access DIVERSITY OF MITE PESTS ON SOME SUMMER VEGETABLE CROPS WITH SPECIAL REFERENCE TO MANAGEMENT OF OKRA MITE (TETRANYCHUS URTICAE KOCH.)(Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, 2016) Koul, Kuldeep; Singh, Amit KumarExperimentation for management of okra mite (Tetranychus urticae Koch.) on okra was conducted at Chatha farm, SKUAST- Jammu from February 2015 till the harvest of crop (July, 2015). In the present investigation, 10 different vegetable crops were surveyed for the diversity of mite pests and recorded five different types of phytophagous mite species such as two-spotted spider mite, Tetranychus urticae Koch; Tetranychus neoealidonicus Andre; Polyphagotarsonemus latus Banks; Red-legged spider mite, Tetranychus ludeni Zacher; and Spider mite, Tetranychus macfarlanei Baker & Pritchard. Apart from phytophagous mites, one predatory mite viz., Amblyseius tetranychivorus (Gupta) was also recorded feeding on okra mite. Further, seven other predatory fauna on Tetranychus urticae mite on okra such as Coccinellids beetles (Coccinella septempunctata and C. sexmaculata); Small black beetle (Stethorus sp.); Rove beetle (Paederus spp.); Green lace wing, Chrysoperla carnea; Spiders (Oxyopes spp. and others); and minute pirate bug (Orius spp.) were also observed in the okra field. The seasonal incidence of okra mite observed at experimental sites indicated that okra mite, T. urticae was found damaging and remain active on the okra crop and attained two peaks i.e., in 18th standard week (44.13 mean number of mites per 2.5 cm2) and in in 25th SW (55.53 mean number of mites population/2.5 cm2), respectively. The increase in temperature coupled with decrease in relative humidity from March 2015 to June 2015 favoured the increase of T. urticae Koch. The correlation studies showed that the highly significant and positively correlated with maximum temperature had existed with okra mite whereas, negatively correlated with relative humidity (highly significant with morning R.H and significant with evening R. H) and negatively correlated and significant with rainfall. Predatory mite population build-up observed on okra crop had two peaks viz., in 19th SW (5.00 mite/leaf) and again in 27th SW (8.67 mite/leaf). Predatory mite population was positively correlated and highly significant with minimum temperature and significant with maximum temperature, respectively. Predation study revealed that A. tetranychivorus (Gupta) prefer the egg stage of prey than larvae, nymph and adult. Mean consumption rate of A. tetranychivorus (Gupta) on T. urticae egg was found to be 4.16 ± 0.33 per day per gravid female. Bio-efficacy of certain acaricides/ botanicals / allelochemicals / insecticide against okra mite in laboratory condition Bio-efficacy of certain acaricides/ botanicals / allelochemicals / insecticide against okra mite was recorded after two spray (1st and 2nd spray). After 14th days of 1st spray, Abamectin was found to be the best treatment in reducing the mites population (74.64 per cent) on okra followed by Diafenthiuron (68.02 per cent) and Fenazaquin (60.43. per cent). Among the biopesticides, pongamia oil gave 46.32 per cent reduction followed by neem oil application resulted in only 42.68 per cent reduction in mites population at 7 days after second spray. The present investigation shall be useful in monitoring the mite pest population by encouraging the predatory mites and devising the sustainable Integrated Pest Management strategies against emerging mite pests of summer vegetable crops.ThesisItem Open Access Economic analysis of technological change in milk production in Karnal district of Haryana(NDRI, Karnal, 2001) Singh, Amit Kumar; Singh, SurendraThesisItem Open Access EFFECT OF INTEGRATED NUTRIENT MANAGEMENT ON THE PERFORMANCE OF GREEN GRAM (Vigna radiata L.) AND BLACK GRAM (Vigna mungo L.) GENOTYPES(Banda University of Agriculture & Technology, Banda210001, Uttar Pradesh, India, 2022-07-21) Pandey, Ved Prakash; Singh, Amit KumarI have worked on the topic referred above under the supervision of Dr. Amit Kumar Singh. The experiment was conducted in a split-plot design at the College Research Farm of BUAT, Banda during the Kharif season of 2021. The holistic aim of t his ex perim ent w as to search out the most suitable variety of Green gram and Black gram that could easily produce the optimum yield with better profitability and also to standardize appropriate nutrient management techniques that suit well to the crops and variety in the region. The experiment consisted of two-factor treatments with 4 levels. Therefore, a total of 16 treatments combination arrived to test, and every treatment(s) was/were replicated thrice in an experimental unit. The main plot consisted of the nutrient management techniques that were applied to the tested crops and their varieties namely, Farmers’ Practice (FP), Recommended dose of Fertilizers (RDF), 50% Recommended doe of phosphorus + 5.0 quintal biofertilizer carrier-based enriched farm yard manure (EFYM) and 25% RDP + 10.0 quintal EFYM. Further, each main plot had divided into 4 sub-plots held 2 crops with their 2 varieties namely Varsha, Kanika of Mung bean and IPU 11-02, IPU 2-43 of Urd bean. In general, the weather condition was quite conducive resulting in harnessing good crop yields during the year of field experimentation. The soils of the experimental unit were silty clayey and had moderate a pH of 7.6 and EC 0.16 dSm-1 . It was poorly fertile having total organic carbon (11.9 g kg-1 ) and nitrogen (2240 kg ha-1 ) and available phosphorus (17.2 kg ha-1 ) and potassium (257.3 kg ha-1 ). As per the standard package and practices for the Urdbean/Mungbean were applied and accordingly, the experimental field was prepared and later sowing of treated seeds was done at a spacing of 45*10 cm apart. Observations on growth parameters and quantitative and qualitative attributes of Mung and Urd bean were recorded as and when required. The conducted field study during Kharif 2021 under rain-fed late sown condition reveals that Urd bean variety “IPU 11-.02” when sown on July 16th was performed significantly superior over other varieties and crops tested irrespective of integrated nutrient management practices applied. The integrated application of nutrients when applied as basal 50% RDP accompanied with Biofertilizer carrier-based enriched FYM @ 5 q ha-1 proved distinct in terms of growth and development that resulted in maximizing yield productivity, and profitability of the crops. Nonetheless, an Urdbean variety “IPU 11-02” was tested with either 50% RDP + 5 quintals EFYM or 25% RDP + 10 quintal EFYM among the treatment combinations, which produced a statistically similar response concerning major growth and yield attributes and ultimately resulted in identical yields. Among the nutrient management, crop and variety studied, the application of 50% RDP accompanied with Biofertilizers carrier-based enriched FYM @ 5 q ha-1 to Urd bean var. IPU 11-02 produced the maximum seed yield and monetary advantage. Therefore, a holistic technology is recommended for the pulse growers of the Bundelkhand area, this technology stands practically feasible and economically viable.ThesisItem Open Access EFFECT OF PRE AND POST EMERGENCE HERBICIDES ON WEED DYNAMICS, GROWTH, YIELD AND ECONOMICS OF BLACK GRAM (Vigna mungo L.)(Banda University of Agriculture & Technology, Banda-210001, Uttar Pradesh, India, 2020-11-21) Kumar, Vivek; Singh, Amit KumarThe field experiment was carried out at the Agricultural farm of Banda University of Agriculture and Technology, Banda on the prescribed topic "Effect of pre and post-emergence herbicides on weed dynamics, growth, yield, and economics of black gram (Vigna mungo L.)" during Kharif season of the year 2019. The experiment was laid out in a randomized block design with three replications and 10 treatments in a clayey loam having soil pH 7.7. The treatments consist of pre-emergence herbicides namely, Pendimethalin @ 0.75 kg a.i./ha and Imazethapyr @ 0.06 kg a.i./ha and post-emergence herbicides viz. Quizalofop-p-ethyl @ 0.05 kg a.i./ha, Halosufuron @ 27 g a.i./ha and Fenoxaprop-p-ethyl @ 0.10 kg a.i./ha applied at 25 DAS along with two checks i.e., weed free and weedy check (Hand weeding). During the field study, it was observed that prominent weed flora Cyperus rotundus (L.), Cynodon dactylon (L.), Saccharum spontaneum (L.), Phyllanthus niruri (L.), Digera arvensis, Ziziphus nummularia, Abelmoschus moschatus, Commelina benghalensis (L.) and Vigna radiata var. sublobata etc. were noticed and their effective and hasty control was also realized. The results revealed that weed-free treatment remained the best though found at par with herbicide layering of different molecules. Therefore, the simplest conclusion can be drawn from the present field study that herbicide layering of pre and post-emergent molecules namely, Pendimethalin @ 0.75 kg a.i.at 2 DAS followed by Quizalofop-p-ethyl@ 0.05 kg a.i. at 20 DAS per hectare was found the most effective and produced greater seed yield (1146 kg/ha), the total biomass of 3660 kg/ha, net returns (Rs. 50383/ha), and B:C ratio (3.28:1). However, weed-free produced the maximum seed yield of 1229 kg/ha but failed to be remunerative due to the intensive engagement of labour during two hand weedings at 20 DAS and 40 DAS. Thus generated technology holds promise for on-farm adoption for enhancing the yield productivity by minimizing weeds in terms of better weed control efficiency (77.89%) and weed index (6.67%) during Kharif season under the prevailing climatic conditions of Bundelkhand in general and Banda in particular.ThesisItem Open Access Feasibility studies on manufacture of basundi using three stage scraped surface heat exchanger.(NDRI, Karnal, 2009) Singh, Amit Kumar; Dodeja, A.K.ThesisItem Open Access Feasibility studies on manufacture of basundi using three stage scraped surface heat exchanger.(NDRI, Karnal, 2009) Singh, Amit Kumar; Dodeja, A.K.ThesisItem Open Access Insect Pest Succession and their Abundance on Pecan nut, Carya illinoinensis (Wangenh.) Koch(Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, 2022-11) Farid, Marya; Singh, Amit KumarThe pecan nut Caryaillinoinensis(Wangenh.)Kochacknowledged as the ‘Queen of Nuts’ is one of the most valuable nut crops grown internationally owing to its distinct flavour and ideal dietary content. However, the production and productivity of this cherished fruit crop has been threatened all over the world due to various biotic factors, especially insect pests leading to colossal monetary losses. In the Union Territory of Jammu and Kashmir, no study has ever been conducted to study the pest fauna of this nut crop. Based on this conception, the present study entitled, "Insect pest succession and their abundance on Pecan nut, Caryaillinoinensis(Wangenh.) Koch" was intended to fill this gap and epitomize an exhaustive inventory of different insect pests associated with this crop in the twin districts of Poonch and Rajouri, where it is grown. Sampling was done on weekly basis in both the districts and insects were collected based on the visual observation and captured employing sweeping nets, beating and hand picking from the plantations not sprayed with any insecticide. The population build-up of different pests mostly occurred during the months of May, June and July. The results of the present investigation indicated that the pecan plants are attractive to a wide variety of insect pests in both the districts at different study sites. In total, 10 insect species including the beneficials belonging to 4 orders and 8 families were recorded and identified. A comparison of the entomofauna of young and mature pecan plants indicated that insect pest species were more diversified on mature pecan plants than on young especially in the Poonch district. Among them, Phyllophaga sp., Hieroglyphus banian andMyllocerusundecimpustulatusappeared as pests of economic significance requiring chemical control. Beneficial insects encountered during the study included Mantis religiosa, Exochomusnigripennisand Camponotusdiscolor.The results of the current investigation present an important baseline for the design and implementation of management strategies of economically important pests of pecans coupled with the exploitation of natural enemies especially in Poonch district.ThesisItem Open Access Marketing of hybrid paddy seed and its future prospects of Annapurna seeds in Ranchi district of Jharkhand(jnkvv, jabalpur, 2007) Singh, Amit Kumar; Raghuwanshi, N. K.ThesisItem Open Access Population Dynamics and Management of Fall Armyworm, Spodoptera frugiperda (J.E. Smith)(Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu, 2024-05-20) Singh, Monika; Singh, Amit KumarThe thesis embodies the experimental research conducted over two years (2021 and 2022) at the Entomology Farm, SKUAST-Jammu, focusing on the “Population Dynamics and Management of Fall Armyworm, Spodoptera frugiperda (J.E. Smith)”. The study encompassed fortnightly surveys across three distinct areas in Jammu district viz., Nagrota, Marh and R.S. Pura Block, to assess the host range and status of S. frugiperda and its natural enemies. The findings revealed widespread fall armyworm infestation, particularly in maize, with minor infestations observed in chilli, cabbage and sugarcane. In the maize ecosystem, ten natural enemies, comprising predators and parasitoids of fall armyworm, were identified. Population dynamics analysis indicated the emergence of adult fall armyworm starting from the 18th standard meteorological week (SMW), with first peak trap catches occurring in the 24th SMW during both years, followed by a second peak in 28th and 27th SMW during 2021 and 2022, respectively after which the population declined till 31st SMW. Similarly, the larval population of fall armyworm commenced from 20th SMW which attained the first peak population in 25th and 26th SMW in 2021 and 2022, subsequently the 2nd peak was observed in 28th and 29th SMW during 2021 and 2022, respectively. Thereafter, the larval population witnessed a gradual decline and observed the minimum population during 31st SMW. Comparative studies between protected and unprotected plots demonstrated higher fall armyworm larval densities in unprotected areas, leading to increased leaf and ear damage and lower yields than the protected plots, and the calculated avoidable yield losses was 33.85 per cent. Among the different management modules evaluated, chemical intensive management exhibited the lowest mean fall armyworm larval counts (0.17 and 0.18 per plant during 2021 and 2022, respectively), followed by IPM, IIMR recommendation, and bio-intensive modules while the control plots showing the highest infestation levels (2.01 and 2.13 per plant during 2021 and 2022, respectively). Evaluating the economic viability of management strategies, the chemical intensive module yielded the highest net profit and cost-benefit ratio (Rs. 25247.00/hectare; 1:2.16), followed closely by IPM (Rs. 21470.00/hectare; 1:1.81), IIMR recommendation (Rs. 19422.00/hectare; 1:1.79), and bio-intensive modules (Rs. 8917.00/hectare; 1:1.48). The present result revealed that IPM module behaved at par with chemical module but it has being advocated that IPM module will be preferred over the chemical module.ThesisItem Open Access SEASONAL INCIDENCE OF MAJOR PESTS OF COWPEA, VIGNA UNGICULATA (L.) WALP. WITH SPECIAL REFERENCE TO MANAGEMENT OF MITE PEST(Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, 2016) Raina, Shallu; Singh, Amit KumarThe present experiment on seasonal incidence of major insect pests of cowpea, Vigna unguiculata (L.) Walp. with special reference to management of mite pest was conducted at Chatha farm, SKUAST- Jammu from February 2016 till the harvest of crop (June, 2016). The study revealed that bean mite (Tetranychus ludeni), thrips (Megaulothrips spp) and blue butterfly (Lampides boeticus L.) are the major insect pest of cowpea. Infestation of T. ludeni was first observed nearly a month after seed sowing i.e., The seasonal incidence of bean mite observed at experimental sites indicated that bean mite, T. ludeni was found damaging and remain active on the bean crop and attained two peaks i.e., in 20th standard week (52.0 mean number of mites per leaf) and in in 22th SW (58.3 mean number of mites population/leaf), respectively. The seasonal incidence of tetranychid mites varies with the species, climate and the host plant. It is evident from the data that mite population is significantly influence by maximum temperature and relative humidity. The increase in temperature coupled with decrease in relative humidity from 3rd week of March 2016 to 4th week of May 2016 favoured the increase of T. ludeni Zacher. The seasonal incidence of thrips observed at experimental sites indicated that the initial mean number of thrips population (0.33 per plant) was recorded in the 11th SW and peak population was observed in 4th week of April to 1st week of May with gradually fluctuated with the aberration in environmental condition and reached the maximum mean population in 21st SW (17.00 per plant). Thereafter, the thrips population was declining due to increase in temperature. The seasonal incidence of blue butterfly was observed at experimental sites indicated that the initial mean number of larval population (0.33 per plant) was recorded in the 10th SW and peak population was observed in April to June with gradually increasing and reached the maximum mean population in 23rd SW(17.67 per m2). Thereafter, the larval population was declining due to maturity of crop. The weather parameters i.e temperature (Maximum and Minimum), relative humidity(Morning and Evening) and rainfall, plays an important role in reproduction, development and survival of the insect pests. Weather parameters had greater influence on fluctuation of mite population. The correlation studies showed that the highly significant and positively correlated with maximum temperature had existed with bean mite whereas, negatively correlated with relative humidity (highly significant with morning R.H and significant with evening R. H) and negatively correlated and significant with rainfall. The correlation studies showed that the broad mite populations was highly significant and positively correlated with maximum and minimum temperature whereas, It was negatively correlated with relative humidity morning and evening with correlation values of -0.772 and -0.685, respectively. However, a negative and non-significant correlation was established with rainfall. The overall impact of weather factors on population build-up of mite was 69.70 per cent which reflects that there were some another environmental factors i.e. solar radiation, sunshine, rainy days and wind velocity who governed the 30.30 per cent role in population build-up of bean mites on cowpea. A significant positive correlation existed between weekly temperature (highly significant with maximum temperature and significant with minimum temperature) and thrips and blue butterfly population on Cowpea whereas, pest population build up was found to be significant and negatively correlated with relative humidity (highly significant with morning R.H and significant with evening R.H) and negatively correlated and significant with rainfall. The corresponding correlation co-efficient of multiple determination (R2) values worked out to be 0.931 and 0.961 for thrips and blue butterfly, respectively. A single unit increase in temperature (maximum and minimum), relative humidity (morning and evening) increases the blue butterfly larval population. Among the weather factor, temperature is a most important factor affecting insect growth, development rate, and survival of Megaulothrips spp. Results from this study indicated that the mean number of Megaulothrips spp. declined with increasing temperature. After fourteen days of first spray, the best effect was given by abamectin1.8 EC which reduced the bean mite population by 70.32 per cent. This was followed by the treatment of spiromesifen in different plots, causing a reduction of 60.43 per cent. On the other hand application of fenpyroximate resulted in 58.68 per cent reduction. The biopesticide (pongamia oil) gave 49.58 per cent reduction in mite population at 7 days after second spray. Overall, abamectin is the most effective treatment after two sprays up to 14th days in reducing the mites population and found to be significantly different from spiromesifen and fenpyroximate at 5 per cent level of significance. After fourteen days of both sprays, the best effect was given by chlorfenapyr 10 EC which reduced the thrips population by 58.24 to 60.88 per cent, respectively. This was followed by the treatment of imidacloprid in different plots, causing a reduction of 48.04 per cent in first spray however 49.58 per cent in second spray. The present investigation shall be useful in monitoring the mite pest population by encouraging the predatory mites and devising the sustainable Integrated Pest Management strategies against emerging mite pests of summer vegetable crops.ThesisItem Open Access Studies on handmade somatic cell cloning in guffalo (Bubalus bubalis).(NDRI, Karnal, 2006) Singh, Amit Kumar; Singla, S KThesisItem Open Access STUDIES ON THE IMMUNOPATHOLOGY OF AFLATOXICOSIS IN DUCKS(Indira Gandhi Krishi Vishwa Vidyalaya, Raipur, 2006-11) Singh, Amit Kumar; Ghosh, R.C.; Jogi, Sudhakar; Vadlamudi, V.P.; Hirpurkar, S.D.; Gupta, NeeluSTUDIES ON THE IMMUNOPATHOLOGY OF AFLATOXICOSIS IN DUCKSThesisItem Open Access STUDIES ON THE IMMUNOPATHOLOGY OF AFLATOXICOSIS OF DUCKS(CHHATTISGARH KAMDHENU VISHWAVIDYALAYA, DURG, 2006) Singh, Amit Kumar; Ghosh, Dr. R.C.ThesisItem Open Access Study on Impact of Herbal Disinfectantsand Protein Supplements on Quality of Silk Fibre(Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu, 2023-09-19) Kamboj, Lovekesh; Singh, Amit KumarBombyx moriL. (Lepidoptera), commonly known as mulberry silkworm reared on the leaves of mulberry for large scale production of silk. The fortification of mulberry leaves withprotein supplements is an emerging technique in advanced sericulture research for enhancing the quality and quantity of silk. Therefore, two different concentration of protein supplements were prepared and administered to the silkworm larvae and herbal and commercial bed disinfectants were also applied on the silkworm larvae for the production of better-quality silk during the research. The study was conducted in the sericulture research laboratory of the Division of Sericulture, SKUAST-J.All the protein supplements and herbal disinfectants along with the traditional disinfectants proved better. However, among various treatments,protein supplement I recorded significantly the highest larval weight (42.38 ±0.38)g and survival (97.48± 0.53%)which was followed by T4 i.e., protein supplement II. Moreover, the cocoon yield by weight (g) and number;(14.09± 0.50) and (9165 ±34.35) were found to be the highest in T3 followed by T4i.e. (12.93±0.20) and (9113±40.96). However, the respective values were the lowest in control (9.90) and (7790±76.38).The revealed significant increase in good cocoon percentage from (56.12 ±1.48%) (control) to (83.67± 1.78%) and (85.89± 0.26%)in T4and T3, respectively. Simultaneously, the pupation percentage among the various supplements increased from (57.08±1.20%) in control to (84.18±1.09%) in T4 to (86.40± 0.60%) in T3. It was also found that the single cocoon weight, shell weight and shell ratio percentage were significantly the highest in T3i.e., (1.60±0.01)g, (0.33±0.02)g,(20.01±0.10%)followed by T4viz., (1.52±0.02), (0.30±0.01)g and (19.73±0.06%),respectively. However, the lowest values for single cocoon weight (1.25±0.02)g, single shell weight (0.23±0.01)g and shell ratio percentage (18.48±0.11%)were recorded in control. The different treatments showed significant influence on the cocoon length and cocoon width i.e., (35.176 ±0.28)mm and (20.126±0.09)mm in T3, (33.090±0.08)mm and (19.150±0.10)mm in T4 followed by (26.700±0.69) mm and (16.200±0.29)mm in control. The study also revealed that the filament size (denier) was found to be the lowest (2.000±0.05)g in T3 and the highest (2.846±0.03)g in control.ThesisItem Open Access VERIFICATION OF PARENTAL LINES AND HYBRIDS THROUGH MOLECULAR MARKERS IN BRINJAL (Solanum melongena L.)(Indira Gandhi Krishi Vishwavidyalaya, Raipur, 2011) Singh, Amit Kumar; Mehta, Nandan; Saxena, (Smt.) Ritu R.; Shukla, Neeraj; Saxena, R.R.The present investigation was undertaken during Rabi-2010 and Summer-2011. The experimental materials were planted at Horticultural Research Farm, All India Coordinated Vegetable Improvement Project, Department of Genetics and Plant Breeding, Indira Gandhi Krishi Vishwavidyalaya, Raipur (C.G.). The experiment was subject to identify different parental lines; test the hybridity and to observe mean performance of parents and hybrids. The experimental materials used in the study comprised of seven parents viz., Green Long (GL), Muktakeshi (MK), Pusa Purple long (PPL), IBWL-2007-1 (IBWL), Pusa Purple Cluster (PPC), Pant Rituraj (PR), Panjab Sadabahar (PS) and their sixteen F1‟s. Two molecular markers viz., RAPD and ISSR were used to fulfill the objectives. To study the mean performance of parents and hybrids the observations were collected on days to 50% flowering, days to first picking, plant height, number of primary branches per plant, average fruit length (cm), average fruit girth (cm), average fruit weight (g), total number of fruits per plant, number of fruits per plant per picking, marketable fruit yield per plant, total fruit yield per plant and total soluble solids (%). For the parental identification and hybridity test a total of forty-nine RAPD and thirteen ISSR primers were screened. Out of which, only two RAPD primers OPA-10 and OPH-20 and only one ISSR primer i.e., UBC-808 was found to be polymorphic among the parents. For the primer OPH-20, Pusa Purple Cluster and Panjab Sadabahar showed polymorphism and for the primer OPA-10, the parent Green Long and Pusa Purple Cluster was polymorphic. A hybrid between P2 and P7 (Green Long x Panjab Sadabahar) was identified on the basis of banding pattern which resembles to female parent Green Long (P2). In another hybrid of P5 and P6 (Pusa Purple Long x Pusa Purple Cluster) banding pattern of F1 hybrid resembles to one parent Pusa Purple Long (P5). F1 hybrid of parent P6 and P1 (Pusa Purple Cluster x IBWL-2007-1), the banding pattern resembles to one parent i.e, Pusa Purple Cluster (P6). F1 hybrid, P3 and P2 (Muktakeshi x Green Long), the banding pattern found to be similar to male parent Green Long. For the primer OPH-20, an F1 hybrid between P5 and P6 (Pusa Purple Long x Pusa Purple Cluster) produced banding pattern which resembles to male parent Pusa Purple Cluster. For the ISSR primer UBC-808, the banding pattern of the hybrid (Pusa Purple Cluster x IBWL-2007-1) resembles to male parent i.e. IBWL-2007-1. In genetic diversity analysis of seven genotypes of brinjal it is found that genotype P3 (Muktakeshi) and P7 (Punjab Sadabahar) are very closely related with genetic similarity of 85 per cent and the genotype P2 (Green Long) forms a separate cluster which means that genotype P2 (Green Long) is extremely different form rest the all genotypes. The mean performance study of parents and hybrids, resulted that among the parent Green Long gave the highest yield potential, followed by IBWL and PPC (Pusa Purple Cluster). In case of hybrids it was recorded highest in GL x PPL, which is followed by GL x PS, and PPL x PPC.