Browsing by Author "Sharma, Sheetal"
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ThesisItem Open Access Effect of irrigation management and planting methods on broccoli (Brassica oleracea var.italica) under stored rain water resource(CSKHPKV, Palampur, 2004) Sharma, Sheetal; Masand, S.S.ThesisItem Open Access Effect Of Vegetation On Runoff Production And Utilization Of Stored Water To Evolve Profitable Vegetable Crop Sequences(CSK Himachal Pradesh Krishi Vishvavidyalaya;Palampur, 2008) Sharma, Sheetal; Kapur, O.C.; CSK Himachal Pradesh Krishi Vishvavidyalaya; PalampurThesisItem Open Access Prevalence and antimicrobial resistance of Enterococcus spp. and Acinetobacter spp. in pet animals(Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 2022) Sharma, Sheetal; Arora, A.K.In the present study a total of 100 samples (rectal swab) collected for isolation and identification of Enterococcus spp. Eighty (70 from dog, 10 from cats) samples were collected from diseased animal and 20 (18 from dog, 2 from cat) sample were collected from apparently healthy animals. The isolates which found positive from diseased animals were 68 and the isolates which found positive from apparently healthy animals were 10. Antibiogram against 23 antibiotics indicate a high resistance against erythromycin i.e. 83.33%, co-trimoxazole (75.64%), ciprofloxacin (71.79%). Resistance to vancomycin was found 28.57% in E. fecalis and 35% in E. faecium in the isolates taken from diseased animals whereas none of the isolate was found resistant to vancomycin in both the species in isolates taken from healthy animals. Resistance to linezolid and teicoplanin was found 20.51% and 8.94% respectively. Resistance to HLG was found 17.94% while resistace to HLS was found 25.64%. 93% of Enterococcus species were multidrug resistant. Forty two isolates were found as Vancomycin resistant and among them 2 were carried van B gene. Enterococci isolates were also found to carry tet A, tetB, ermB, ermC, aadA genes. For isolation of Acinetobacter spp., a total of 160 samples (urine, blood, skin sample, wound) were collected from pet animals. Eight of the isolates (6 from urine, 1 from blood, 1 from wound) were found positive for Acinetobacter. Antibiogram against 26 antibiotics in Acinetobacter spp. revealed highest resistance against Amoxycillin/Clavulanc acid, erythromycin and tetracyclin i.e. 87.5%. None of the isolates was found resistant to colistin. Three of the isolates were found positive for ESBL production. blaOXA51 gene was used as confirmation for A. baumannii. Int I gene was detected in all isolates. ermB, ermC, qnrD genes were present in 75% of isolates. blaOXA51, NDM-1, ampC, tetA genes were present in 62.5% of isolates. The result indicate that dogs can be considered as a potential reservoir of multidrug resistant A. baumannii and Enterococcus spp.