Browsing by Author "SUSHMA, R. E."

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    CERTAIN STUDIES ON FELINE HAEMOTROPIC MYCOPLASMOSIS
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2022) SUSHMA, R. E.; P. T. RAMESH
    The present study “Certain studies on Feline Haemotropic Mycoplasmosis” was designed to investigate epidemiological aspects, clinical and haemato-biochemical profile associated with feline haemotropic mycoplasmosis and its molecular diagnosis. Among the cats presented to Veterinary College Hospital, Bengaluru during the period from July 2020 to June 2021, 78 anaemic cats were suspected for Haemotropic Mycoplasmosis and were included for detailed study. Peripheral blood smear stained with Giemsa stain were examined for primary identification of organisms. Among 78 cats screened, 12 were positive for haemoplasma by blood smear examination. Most frequent clinical signs noticed in affected cats were pale mucous membranes, anorexia, lethargy, pyrexia, weight loss and splenomegaly. Haematological studies revealed reduction in RBC, haemoglobin and haematocrit values. Biochemical alterations were Hyperglobulinaemia and elevated total protein level. Higher occurrence was observed in summer season and among adult cats, male, non-descript cats with semi-outdoor access. The blood samples were subjected to PCR for amplification of 16S rRNA gene of haemoplasma. The occurrence of Mycoplasma haemofelis and Candidatus Mycoplasma haemominutum was five and thirteen per cent, respectively. Phylogenetic analysis of Mycoplasma haemofelis derived from the hemoplasma infected cats showed high sequence identity to M. haemocanis sequences in Genbank and Candidatus Mycoplasma haemominutum showed 98 – 99 per cent sequence identity to Candidatus Mycoplasma haemominutum isolates of domestic cats from USA, Trinidad, Hungry, Italy, Yamaguchi, Brazil and Thaiwan. Statistically significant reduction in plasma reduced glutathione concentration was seen and no statistically significant difference in IgG quantity was observed between healthy and infected groups of cats. Key Words: Feline Haemotropic Mycoplasmosis, Blood smear, Polylerase Chain Reaction. Annexures