Browsing by Author "SANDEEP"
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ThesisItem Open Access EFFECT OF ORGANIC AND INORGANIC SOURCES OF NUTRITION ON SORGHUM + PEARL MILLET FODDER PRODUCTION, ASSOCIATED WEED FLORA AND SOIL PROPERTIES(CSKHPKV. Palampur, 2020-09-10) SANDEEP; Naveen, KumarA field experiment “Effect of organic and inorganic sources of nutrition on sorghum + pearl millet fodder production, associated weed flora and soil properties” was conducted at Research Farm of Fodder Section, CSKHPKV, Palampur during Kharif 2019. Soil of the experimental field was acidic in reaction, high in organic carbon, low to medium in available nitrogen, medium in available phosphorus and medium in available potassium. The experiment was laid out in randomized block design with three replications, consisting of nine treatments having eight combinations of two nitrogen levels (no nitrogen and 50 per cent of recommended nitrogen), two FYM levels (10 and 15 t/ha) and two levels of Jeevamrit concentration (5 and 10 per cent), plus one treatment of recommended dose of NPK (120, 60, 40 kg/ha) through inorganic sources. Treatments comprised of integrated nutrient management (50 per cent recommended N + FYM + Jeevamrit) and recommended NPK behaving alike resulted in better crop growth (emergence count, plant height, shoot number, leaf stem ratio, dry matter accumulation), fodder yields (green and dry), crude protein content, crude protein yield and lower weed population and weed dry matter accumulation compared to organically managed systems with variable levels and concentration of FYM and Jeevamrit, respectively. The uptake of N, P and K by crops was significantly higher with 50 per cent recommended N + 15 t/ha FYM + 10 per cent Jeevamrit. Application of recommended NPK proved most profitable with highest net return (₹ 59,018/ha) and benefit-cost ratio (2.75). Treatments with integrated use of 50 per cent recommended N + FYM and Jeevamrit increased available soil nitrogen, phosphorus and potassium over initial values and increase was highest with recommended NPK. The soil organic carbon and microbial biomass carbon were highest with 50 per cent recommended N +15 t/ha FYM + 10 per cent Jeevamrit.ThesisItem Open Access PLANT REGENERATION VIA ORGANOGENESIS FROM MATURE TISSUES OF CITRUS ROOTSTOCK HYBRIDS(DIVISION OF FRUITS AND HORTICULTURAL TECHNOLOGY INDIAN AGRICULTURAL RESEARCH INSTITUTE NEW DELHI, 2019) SANDEEP; A. K. DubeyIn the current work, efforts were made to examine culture of nodal explants derived from the mature tissues of field grown two citrus rootstock hybrids CRH-18-2/15 (RLC-6 x pomeroy) and CRH-20-2/15 (small fruited mandarin x Troyer citrange). Additionally, effect of explant type, media supplement and ethylene inhibitors on shoot and root induction were evaluated in citrus rootstock hybrid CRH-18-2/15. Also tested clonal homogeneity of in vitro raised plants using Simple Sequence Repeat (SSR) markers. Results on basic media, explant type and genotype exhibited higher responsive explants (100%) and bud proliferation rate (97.78% and 95.56%) when basal and apical zone segments of CRH-20-2/15 were cultured on DKW medium, but in hybrid CRH18-2/15, higher responsive plants (100%) and bud proliferation (100% and 98.33%) was observed while apical and basal nodal segments inoculated on MT and GB-5 media, respectively. In CRH-20-2/15, highest shoot length (17.67 mm) was measured with middle zone segments when cultured on MT medium, while in other hybrid, higher shoot length was found when middle zone segment cultured either on MT (10.67 mm) or DKW (8.00 mm) medium. In fact, number of leaves/ regenerated explant in CRH20-2/15 hybrid was counted more with middle zone segments inoculated on MT medium but in CRH-18-2/15, it was found higher with apical and basal zone segment while cultured on DKW medium. Earliest shoot induction at 6th day of inoculation was observed with all nodal zones, while cultured on MT medium CRH-20-2/15, though shoot induction was completed within 15 days after inoculation. Media supplement also indicated significant difference in shoot induction of hybrid CRH-18-2/15. DKW medium supplemented either with 2ip or BA along with GA3 and NAA produced satisfactorily results for shoot induction. Higher bud proliferation rate (91.54% and 73.97%) was noticed with apical and middle zone segments, respectively, while cultured on DKW medium supplemented with BA, GA3 and NAA. More number of regenerated explants were found with CoCl2, while silver thiosulfate promoted bud proliferation rate, shoot length and number of buds per regenerated explant. Combined application of both CoCl2 and STS was found to be toxic for shoot induction in hybrid CRH-18-2/15. As judged by SSR primers, all the in vitro raised plants showed 100% uniformity among themselves and with the mother plant. 92 Keywords: Regeneration, explant, basic media, segment