Browsing by Author "SAGAR SHANKAR VYAVAHARE"
Now showing 1 - 2 of 2
Results Per Page
Sort Options
ThesisItem Open Access COMPARATIVE ANALYSIS OF INTRADERMAL TUBERCULIN TEST AND γ- INTERFERON ASSAY FOR DIAGNOSIS OF BOVINE TUBERCULOSIS(Anand Agricultural University, Anand, 2012) SAGAR SHANKAR VYAVAHARE; Dr. M. K. JhalaA comparative study on interferon-γ (IFN-γ) assay, single intradermal tuberculin skin test (TST) and Enzyme linked immunosorbant spot (ELISPOT) for the diagnosis of Bovine tuberculosis was conducted. A total of 252 cattle stationed at a cattle farm were tested for Bovine tuberculosis by TST, IFN-γ assay and 23 of them by ELISPOT analysis. TST was carried out as per OIE Terrestrial Manual (2009) using PPD- Tuberculin. The reaction was considered to be positive if there was an increase of 4 mm or more in skin-fold thickness along with the local signs. Out of 252 cattle tested for Bovine tuberculosis, 66 (26.19%) cattle were found positive by TST. Breedwise, 13 (16.45%) animals of Kankrej (n=79), 30 (38.96%) of Gir (n=77) and 23 (23.95%) of Triple cross (n=96) breed were positive by TST. Agewise, 47 (37.90%) adults (n=124), 11 (11.22%) heifers (n=98) and 8 (26.66%) calves (n= 30) were positive by TST. Sexwise, 18 (56.25%) males (n=32) and 48 (21.81%) females (n=220) were positive by TST. All the 252 cattle at a farm were tested for Bovine tuberculosis by IFN-γ assayThesisItem Open Access COMPARATIVE ANALYSIS OF INTRADERMAL TUBERCULIN TEST AND γ- INTERFERON ASSAY FOR DIAGNOSIS OF BOVINE TUBERCULOSIS(Anand Agricultural University, Anand, 2012) SAGAR SHANKAR VYAVAHARE; Dr. M. K. JhalaA comparative study on interferon-γ (IFN-γ) assay, single intradermal tuberculin skin test (TST) and Enzyme linked immunosorbant spot (ELISPOT) for the diagnosis of Bovine tuberculosis was conducted. A total of 252 cattle stationed at a cattle farm were tested for Bovine tuberculosis by TST, IFN-γ assay and 23 of them by ELISPOT analysis. TST was carried out as per OIE Terrestrial Manual (2009) using PPD- Tuberculin. The reaction was considered to be positive if there was an increase of 4 mm or more in skin-fold thickness along with the local signs. Out of 252 cattle tested for Bovine tuberculosis, 66 (26.19%) cattle were found positive by TST. Breedwise, 13 (16.45%) animals of Kankrej (n=79), 30 (38.96%) of Gir (n=77) and 23 (23.95%) of Triple cross (n=96) breed were positive by TST. Agewise, 47 (37.90%) adults (n=124), 11 (11.22%) heifers (n=98) and 8 (26.66%) calves (n= 30) were positive by TST. Sexwise, 18 (56.25%) males (n=32) and 48 (21.81%) females