COMPARATIVE ANALYSIS OF INTRADERMAL TUBERCULIN TEST AND γ- INTERFERON ASSAY FOR DIAGNOSIS OF BOVINE TUBERCULOSIS
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Date
2012
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Anand Agricultural University, Anand
Abstract
A comparative study on interferon-γ (IFN-γ) assay, single intradermal tuberculin skin
test (TST) and Enzyme linked immunosorbant spot (ELISPOT) for the diagnosis of Bovine
tuberculosis was conducted. A total of 252 cattle stationed at a cattle farm were tested for
Bovine tuberculosis by TST, IFN-γ assay and 23 of them by ELISPOT analysis. TST was
carried out as per OIE Terrestrial Manual (2009) using PPD- Tuberculin. The reaction was
considered to be positive if there was an increase of 4 mm or more in skin-fold thickness
along with the local signs. Out of 252 cattle tested for Bovine tuberculosis, 66 (26.19%) cattle
were found positive by TST. Breedwise, 13 (16.45%) animals of Kankrej (n=79), 30
(38.96%) of Gir (n=77) and 23 (23.95%) of Triple cross (n=96) breed were positive by TST.
Agewise, 47 (37.90%) adults (n=124), 11 (11.22%) heifers (n=98) and 8 (26.66%) calves (n=
30) were positive by TST. Sexwise, 18 (56.25%) males (n=32) and 48 (21.81%) females
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Keywords
Veterinary Microbiology, Comparative analysis