Browsing by Author "Rajendran, C."
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ThesisItem Open Access Evaluation of recombinant Theileria annulata merozoite surface antigen for serodiagnosis of tropical bovine theileriosis(Indian Veterinary Research Institute;Izatnagar, 2006) Rajendran, C.; Ray, D. D.ArticleItem Open Access Isolation and characterization of Toxoplasma gondii from small ruminants (sheep and goats) in Chennai City, South India(Journal Impact Factor, 2017-07) Satbige, Ajay Suryakant; Sreekumar, C.; Rajendran, C.; Bharathi, M. Vijaya; TANUVASThe present study aimed for the isolation and genotyping of Toxoplasma gondii from small ruminants (sheep and goats). 14 out of 193 tissue samples (either brain and heart) tested positive by MDAT for anti-T. gondii antibodies, were selected and bioassayed, which resulted 4 samples positive for T. gondii after 40 days of post inoculation. Four samples consisting of 3 numbers of sheep and 1 number of goat tissues out of 14 samples detected by B1 PCR, were genotyped at SAG3 locus by nested polymerase chain reaction–restriction fragment length polymorphism technique (nPCR–RFLP). The results of the present study revealed that the four isolates designated as TgShIn19, TgShIn76, TgShIn77 and TgGtIn27 were circulating in small ruminants, were belonged to genotypes of type II (TgShIn19) and type III (TgShIn76, TgShIn77 and TgGtIn27) which are in concordance with the previously reported genotypes from other animal species and further this presumptive results indicating that the genotype II and III could be the predominant in different animal species including birds and humans in India.OtherItem Open Access PCR-RFLP Genotyping of Toxoplasma Gondii from Sheep and Goats from Chennai, Tamil Nadu State: New Genotypes and A New SAG3 Marker Allele(TANUVAS, 2016-02) Satbige, Ajay. S.; Bharathi, M. Vijaya; Sreekumar, C.; Rajendran, C.ArticleItem Open Access Polymerase chain reaction based detection of Toxoplasma gondii from lymph node aspirates of dogs(Journal Impact Factor, 2014-01) Bharathi, M. Vijaya; Sreekumar, C.; Rajendran, C.; TANUVASdogs of different age groups. Lymph node aspirates were analysed with polymerase chain reaction (PCR). Two numbers of dogs were found to be positive by PCR. The PCR positive dogs showed modified direct agglutination test (MDAT) titre of 1:512 and 1:4,096. The remaining sera samples showed the titre value of less than 1:64 byMDAT andwere also negative by PCR. This study indicates that lymph node aspirates could be an alternative ideal specimen for the detection of T. gondii.