Browsing by Author "Raja, P"
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PresentationItem Open Access Complete genome sequence analysis of canine Parvovirus new 2a variant strain isolated from a dog with severe haemarrogic gastroenteritis in South India(2020-02) Raja, P; Parthiban, M; Maha Prabhu, M; et al.; TANUVASThe canine parvovirus (CPV) infection is characterized by severe hemorrhagic gastroenteritis and myocarditis in dogs caused by different antigenic variants CPV-2a, CPV-2b, New CPV-2a, New CPV-2b and CPV-2c and these antigenic variants are evolved rapidly and completely replaced the original CPV-2 variants in throughout the world. In India, there are several reports even in vaccinated dogs with CPV infections in recent years and the predominant type of antigenic variant is CPV-2a. Here, we report a virulent field CPV-2a strain, ABT/CPV/MVC02, was isolated from a faecal sample of a dog with severe haemorrhagic gastroenteritis in Chennai, Tamil Nadu, India. The genome sequence of ABT/CPV/MVC02 was determined and analyzed to study the CPV-2 antigenic variants currently circulating in India. The genomic sequence of ABT/CPV/MVC02 comprises of 4495 nucleotides (nt) containing two open reading frames (ORFs). ORF1 (nt 1-2007) encoded two non structural proteins (NS1 and NS2), and ORF2 (nt 2014-4270) encoded two structural proteins (VP1 and VP2). The genome sequence of ABT/CPV/MVC02 shares 99.33% nucleotide identity with those of CPV new 2a variants (MH476591 and MH476580) strains and shares 99.20% and 98.98% nucleotide identity with JQ268284 (CPV 2b) and KU508407 (CPV 2c) strains respectively. There is one insertion site at VP1 region (nt- 2113) and two deletion sites were found at 3’ untranscribed region (UTR) (nt-4478 and 4481). The present study reveals that the ABT/CPV/MVC02 CPV-2a new variant strain circulating in India is evolving rapidly with unique variations at nucleotide level. Nucleotide sequence accession number: The genome sequence of new CPV-2a strain ABT/CPV/MVC 02 has been deposited in Gen Bank under the accession number MN661243.ArticleItem Open Access CompleteGenome Sequence of a Chicken Embryo Fibroblast - Adapted Attenuated Infectious Bursal Disease Virus Isolate from India(American Society for Microbiology, 2016) Senthilkumar, T.M.A.; Priyadharsini, C.V.; Raja, P; Kumanan, K; TANUVASThesisItem Open Access Development of nucleocapsid gene mediated resistance in tomato against groundnut bud necrosis virus(Indian Agricultural Research Institute;New Delhi, 2005) Raja, P; Jain, R. K.ThesisItem Open Access GENOME ANALYSIS AND GENOME BASED DIAGNOSTICS FOR INFECTIOUS BURSAL DISEASE VIRUS (IBDV)(TANUVAS, 2016) Raja, P; Senthilkumar, TMA; Thangavelu, A; Parthiban, M; TANUVASThe present study was undertaken to analyse the complete genome sequences of the IBDV isolates circulating in the field outbreaks in lndia. This will throw light on the nature of the viruses which cause outbreaks even alter vaccination and to develop strategy for effective vaccination. Further, genome based diagnostics were developed for rapid field level diagnosis of the disease. The bursal tissue samples collected from suspected cases of lBD from different outbreaks in southern states of India were screened by VPI and VP2 gene based RT-PCR.ArticleItem Open Access Genotypic Characterization of Indian Isolates of Infectious Bursal Disease Virus strains by reverse Transcription-Polymerase Chain Reaction Combined with Restriction Fragment Length Polymorphosm Analysis(Acta Virologica, 2016) Priyadharsini, C.V.; Senthilkumar, T.M.A.; Raja, P; Kumanan, K.; TANUVASArticleItem Open Access Metagenomics and their Applications in Veterinary Science(2017-02) Raja, P; Senthilkumar, TMA; PalanisammI, A; TANUVASMetagenomics is analogous approach to the genomics with the difference that it does not deal with the single genome from a clone or microbe cultured or characterized in laboratory It deals with the entire microbial community present in an environmental sample hence it is also called as community genome. The challenge of microbiologists for decades is how to access the microorganisms that cannot be cultured in the laboratory. Ahnost all of our knowledge of microbial life is based on organisms raised in pure culture but metagenomics provides an additional set of tools to study uncultured species. This new field offers an approach to study microbial communities as entire units, without cultivating individual members. Metagenomics entails extraction of DNA from a community and these genomes are usually fragmented and cloned into an organism that can be cultured to create ’metagenomic libraries' and these libraries are then subjected to analysis based on DNA sequence or on functions conferred on the surrogate host by the metagenomic DNA. The metagenomic information allows in-depth understanding of the ecological role, metabolism, and evolutionary history of microbes in a ecosystem. One of the most outstanding discoveries of metagenomics is the first description of proteorhodopsin in marine bacteria. Metagenomics will help in continuous detection and description of novel viruses in the future that leads to a better understanding of both emerging and existing diseases as well as to the increased knowledge of the role of different viruses in humans and animals.ArticleItem Open Access Molecular characterization of canine adenovirus type 2 in dogs from India(2021-04) Raja, P; Sachin, V; Parthiban, M; Aishwarya Janaki, P; TANUVASA total of 26 nasal swab samples were collected from dogs with gastroenteritis and respiratory tract infections in and around Chennai, India during 2019–20. All the samples were subjected to PCR using common primers for rapid diagnosis and differentiation of CAV1 and CAV2. Only one sample produced an amplicon of 1030 bp indicating the presence of CAV2 which was confirmed by further sequencing. The analysis of the sequence revealed 100 per cent identity with other CAV type 2 isolates from Brazilian, Canadian and USA strains and 95.9 per cent identity with other Indian CAV2 strains. The phylogenetic analysis of E3 gene reveal two distinct clusters (Asian and America-Europe subgroup) in which our strain (ABT/ MVC/CAV2/001) grouped with CAV2 of America-Europe subgroup instead of Asian continent subgroup.This study confirms a novel CAV2 strain using molecular techniques which are genetically distinct in nature from other Indian CAV2 strains that is currently circulating in India.ArticleItem Open Access A rare case of congenital renal duplex system with renal calculi in a seventy five years old woman(2020) Jayamurugan, B; Senthil Kumar, P; Raja, P; Subapriya, S; TANUVASUnilateral and bilateral renal calculi are more commonly observed in patients presented with pain in the loin region. Renal duplex system has been encountered occasionally as a congenital anomaly and it is more commonly diagnosed as an incidental finding during intraoperative renal surgical intervention of the patient. The present case is significant as in this case both renal calculi and union of both moieties of the ureters were observed in a seventy five years old woman.ArticleItem Open Access Restriction Fragment Length Polymorphism Analysis of Isolates of Infectious Bursal Disease Viruses from Southern Region of India(2018) Raja, P; Senthilkumar, TMA; Parthiban, M; Thangavelu, A; Mangala Gowri, A; Vijayarani, K; Kumanan, K; TANUVASThe Restriction Fragment Length Polymorphism (RFLP) is used for the differentiation of classical virulent (cv), virulent (v) and very virulent (vv) strains of Infectious Bursal Disease Virus (IBDV) isolates collected from recent outbreaks in southern region of India. In the present study, five different isolates (BGE15, EDE14, RPM14, MDI14 and THI14) of IBDV strains were subjected for genotyping along with vaccine (Georgia, intermediate strain) by performing RT-PCR for amplification of a 743 bp from the hypervariable region of VP2 gene followed by restriction enzyme digestion with seven different enzymes (HhaI, SacI, SspI, StyI, BspMI, StuI and TaqI) for the differentiation of classical, virulent and very virulent strains of IBDV. The RT-PCR product obtained from all the five isolates were not cleaved by SspI and SacI enzyme and thus revealed the absence of restriction enzyme (RE) site for SspI and SacI enzyme, respectively. The HhaI enzyme cleaved vaccine and field isolates with similar restriction profiling pattern. The StuI enzyme did not digest vaccine strain whereas field isolates were cleaved by this enzyme. The BspM1 was not able to differentiate the field isolates from vaccine strain. TaqI enzyme cleaved both vaccine and field isolates of IBDV with different profile pattern. The StyI enzyme showed single RE site on vaccine strain and other field isolates with similar RE pattern. Thus, from the present study, it may be concluded that all the isolates belonged to vvIBDV and they do not have site for SspI marker.