Browsing by Author "Pratheepa, K."
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ArticleItem Open Access Assessment of the Immune Status in “Withaferin A” Treated Rats in Experimental Mammary Carcinogenesis by Flow Cytometry(TANUVAS, 2018-01) Pratheepa, K.; Balachandran, C.; Vijaya, K.; TANUVASThe present study was carried out to evaluate the immunomodulatory effect of Withaferin A in DMBA (7,12-dimethylbenz[a]anthracene) induced mammary carcinogenesis in rats. Twenty four female Sprague-Dawley (SD) rats were equally distributed to control, DMBA, DMBA+Tamoxifen (Standard drug) and DMBA+Withaferin A groups. Peripheral blood was collected at the 120th day from the initial dose of DMBA and CD3, total T lymphocytes were determined by using ow cytometry. There was signi cant (P<0.05) increase in the total T lymphocytes (CD3) in the DMBA+Withaferin A group from DMBA+Tamoxifen group indicating immunostimulatory effect of Withaferin A.ArticleItem Open Access Immunohistochemical Localisation of Cytokeratin and Vimentin Expression in the Experimentally Induced Rat Mammary Tumour(Indian Veterinary Association, 2018-04) Pratheepa, K.; Balachandran, C.; TANUVASThe present study was designed to examine the expression of cytokeratin (CK 8 and 18) and vimentin in DMBA (7,12-dimethylbenz[a] anthracene) induced rat mammary carcinogenesisby immunohistochemical analysis. Twentyfour female Sprague-Dawley rats were equally distributed to form the control, DMBA, tamoxifen (Standard drug) and Withaferin A groups. Mammary tumours were collected on the 120th day after initial dose of DMBA administration. Increased cytoplasmic expression of cytokeratin and vimentin were observed in all the DMBA administered groups and oral administration of Withaferin A did not reduce the tumour burden.ThesisItem Open Access Pathological Evaluation of Anti-Tumour Effects of Withaferin A against Experimentally Induced Mammary Tumour in Rats(TANUVAS, Chennai, 2014) Pratheepa, K.; TANUVAS; Sakthivelan, S.M.; Sridhar, R.; Ramesh, S.The present study was conducted to explore the anti-tumour effect of Withaferin A in DMBA induced mammary tumours in Sprague-Dawley rats. Seventy two rats were equally and randomly distributed to each of the control, DMBA, tamoxifen and Withaferin A groups. Each group contained eighteen rats. The study was conducted for a period of 16 weeks. Animals from DMBA, tamoxifen and Withaferin A groups were administered with 4 weekly doses of 5 mg DMBA/animal in olive oil by oral gavage beginning at the 45th day of age. From the day of first dosing of DMBA, tamoxifen (100 μg/kg BW/Day) was given daily in gingelly oil to tamoxifen group and Withaferin A (16 mg/kg BW/thrice a week) was given thrice a week in PBS (pH 7.4) to Withaferin A group by oral gavage till the end of study