Browsing by Author "Panda, S."
Now showing 1 - 7 of 7
Results Per Page
Sort Options
ThesisItem Open Access Biochemical and molecular analysis of tolerance to iron toxicity in Rice(Department of Agricultural Biotechnology, OUAT, Bhubaneswar, 2020) Palo, Geetanjali; Mohanty, I. C.; Rout, G. R.; Panda, S.Rice, Oryza sativa (2n=24), is the staple food of more than half of the world’s population. It serves as main food for about 36% of the world population. Rice grain contains high nutritional value and calories as compared to other crops and has become an intentional commodity across the whole world. Iron toxicity is one of the most important abiotic stress which limits the rice production in lowland areas (Dobermann& Fair-Hurst,2000). Iron toxicity is common in acid sulfate and water logged condition. The excessive iron uptake causes poor growth and tillering, leaf discoloration (bronzing), and leads to severe yield reductions. In India, it is reported that 43% yield loss in rice is due to iron toxicity. One of the important ways to control the yield loss due to iron toxicity is by developing the new varieties with tolerance to iron toxicity. The research work being reported here was done at the research farm of Regional Research and Technology Transfer station, OUAT, Bhubaneswar. In this present research, a set of 102 rice germplasms including land races and high yielders released for cultivation was evaluated for various morphological characteristics with special reference to identify the iron rich rice genotypes. In a replicated field trail, all the test genotypes were phenotyped using various morphological descriptors both in control and toxicity plot. These phenotypic parameters included the leaf bronzing index (LBI), 50% flowering, number of tillers/ hill, 50% days of panicle initiation, plant height, panicle length, total number of grains/ panicle, 1000 grain weight and yield. All the phenotypic traits were statistically analysed through ANOVA andCV % and CD at (5%) were calculated. Based on LBI score it was revealed that the genotypes like Swarna ,Ganjamgedi , Haribhoga, Mahsuri were tolerant and Hasant , Pratikhsya , Pooja were susceptible to iron toxicity. Out of 102 genotypes, a core population of 45 genotypeswere selected taking 15 genotypes each from tolerant, moderately tolerant and susceptible group for further analysis to study the biochemical and molecular analysis of tolerance to Fe-toxicity.For this purpose,the chlorophyll content with the help of the spadmeter was measured. The grain iron fraction from brown rice of individual genotypes was isolated but the content could not be analysed by Atomic absorption Spectrophotometer due to sudden closed down of the laboratoryowing to COVID-19 pandemic situation. Had the situation been normal, various other biochemical analysis using enzymatic assay and molecular analysis using iron homeostasis related gene specific primers would have been done.ThesisItem Open Access Effect of Metal Nanoparticles on E. Coli Induced Pathology in Broilers(MAFSU, Nagpur, 2020) Panda, S.; Kurkure, N. V.Colibacillosis is a prevalent bacterial disease in the poultry industry throughout the world caused by avian pathogenic Escherichia coli. The multidrug resistance in E. coli strains makes it a potential reservoir for gene resistance possessing zoonotic health risks. To combat the antibiotic resistance alternative techniques for diagnosis and treatment of E. coli are need at the moment. The present research was outlined to elucidate the therapeutic potential of metal nanoparticle mixture as an alternative for the antibiotic resistance in E. coli infection. During the research, the revival of E. coli isolates was done and confirmed by PCR. One of the E. coli isolates positive for both genes was used for challenging the broilers at the concentration of 1x 108 CFU/bird by giving 1 ml inoculum through oral route. A notable difference (P<0.01) in the efficacy of Ag nanoparticles followed by the mixed metal nanoparticle mixture was seen in the in vitro assay as compared with other metals viz. Mg, Cu, Fe, Se, Zn, Cr, Sn. In the present experiment, the addition of metal nanoparticle mixture (1- 28 days) in birds challenged with E.coli infection showed significantly (P<0.01) higher body weight, lower feed intake and comparatively better FCR in respect with other groups. Histopathological lesions in the lungs revealed congestion, haemorrhages, exudates and infiltration of leucocytes. Severe pericarditis and perihepatitis were evident. Peritonitis with distended caeca was observed. Haemorrhages in lamina propria, disruption of villi, sloughing of mucosa along with mononuclear infiltration of cells was found in the intestine. Nonetheless, significant (P<0.01) reduction in intensity of the lesions and bacterial colonization in intestine of N3 fed with nanoparticles from the 10th day of age was observed at 21st day of age. The result of present study indicates that metal nanoparticles can be further explored as an alternative to antibiotic growth promoters.ThesisItem Open Access Genome wide association analysis for tolerance to iron-toxicity in rice(Department of Agricultural Biotechnology, OUAT, Bhubaneswar, 2021) Saha, Debanjana; Mohanty, Iswar Chandra; Rout, G.R.; Pradhan, S.K.; Panda, S.; Bastia, D.N.; Garnayak, L.MRice is one of the main staple food crops and a prominent source of calories for billions of people worldwide. Iron toxicity is a root related abiotic stress; affect the yield of rice in lowland production systems. High concentration of reduced iron (Fe2+) leads to iron toxicity and yield losses due to this varied from 15% to 100% depending on the genotype of rice, growth stage of plant, soil fertility and stress severity. The production of resistant rice genotypes through breeding programs is one of the most promising ways to increase yield under iron toxicity conditions. A powerful way to classify novel loci involved in complex phenotypic characteristics, is Genome-Wide Association Studies (GWASs).The most important aspect is morphological and genotyping characterization, according to this genome mapping method. In this present study, 150 germplasm lines of rice were screened in iron toxicity a hot spot and in control condition as well. Germplasm lines like Ganjamgedi, Kusuma, Pratikhsya, Ranidhan, Lalat etc are found iron toxicity tolerant by LBI scoring (IRRI 2013). Based on the morphological characterization, 120 panel populations have been created for the molecular characterization with 70 SSR markers. A total of 238 alleles were amplified with the 70 SSR markers and average alleles per locus were 4. The highest number of allele was 6 in the marker RM296. Minimum allele frequency was present in RM335 and lowest in RM174.The PIC value was maximum in RM335 and minimum value in RM339. Gene diversity (He) or expected heterozygosity was lowest in RM339 and highest observed in RM335. Estimation of K = 2 based on the ΔK determined by structure harvester, the entire population can be grouped into two sub groups (SP1 and SP2). While considering both the model GLM and MLM, RM346, RM492, RM293, RM201 and RM258 markers are strongly associated with leaf bronzing score. Significant association of RM293 present on chromosome 3 (193.4 cM) designated as qFeTox3.1and RM346 present on chromosome 7 (78.3 cM) designated as qFeTox7.1 was detected as novel QTL for Fe-toxicity tolerance.ThesisItem Open Access Marker validation and introgression of the gene for tolerance to iron toxicity in rice(Department of Agricultural Biotechnology, OUAT, Bhubaneswar, 2020) Behera, Sanjib Kumar; Mohanty, I. C.; Rout, G. R.; Panda, S.; Bastia, D.N.Rice (Oryza sativa L) having chromosome no. 24 belongs to the family poaceae (Gramineae), sub family Bamboosoideae and tribe Oryzeae.It is an Asian originated ,widely important and staple diet of more than fifty percent people of the world, but there are so many biotic and abiotic factors that cause the reduction of the yield of the rice. Among the abiotic stresses iron toxicity is a main problem. Iron toxicity is mainly seen in the water logged area and generally causes 15-30% yield reduction but sometimes it may be severe and cause the entire crop loss in the harsh toxicity at the younger stage. Though there are many methods to control the iron toxicity, advancement of the new variety with tolerance to iron toxicity is the most likely-looking process. Development of the tolerant genotypes using the transgenic methods is a headway process but it is highly denounced due to the biosafety affair. Cis-genic breeding involving introgression of a particular desired trait followed by marker assisted selection is therefore a break-through process for development of high yielding Fe tolerant varieties. Therefore, identifying a closely linked marker that is associated with the trait is the matter of concern. In this research 102 germplasm which include land races and high yielding varieties were grown in the hotspots for the phenotypic assessment regarding the iron toxicity in the regional research and technology transfer station (RRTTS) OUAT, Bhubaneswar. Different parameters were taken for the morphological screening of the total 102 germplasm of the control and toxicity plot each in the two block manners. These phenotypic parameters included the leaf bronzing index (LBI), 50% flowering, number of tillers/ hills, 50% days of panicle initiation, plant height, panicle length, total number of grains/ panicles, 1000 grain weight and yield. The analysis of variance was calculated was calculated and CV % and CD at (5%) was also calculated. Basing upon the average leaf bronzing score it was revealed that their different kinds of plants like highly tolerant, moderately tolerant, tolerant, susceptible and moderately susceptible there. From the previous study and also from this year it was found Swarna, Ganjamgedi Haribhoga, Mashuri, were tolerant to iron toxicity and Hasant, Pratikshya, Pooja, were high yielders but susceptible to iron toxicity. An introgression programme was developed by choosing Hasant and Pratikshya as the female parents and Swarna as the male parents. The F1 seeds thus obtained were again sown in the pot to get F1 plants and from which the genetic analysis could be done and the hybridity test can be conformed using the markers like RM144, RM 456, RM 7102, OsNRAMP5b, RM 217 that would be validated to give contrasting band after PCR reaction previously.Technical ReportItem Open Access Production technology for a high yielding early transplanted rice variety CR Dhan 321(2024) Anandan, A.; Sah, R.P.; Meher, J.; Panda, S.; Bose, Lotan; Pandi, G.G.P.; Keerthana, U.; Annamalai, M.; Kumar, G.A.K.; Nayak, A.K.Technical ReportItem Open Access Production technology for a high yielding early transplanted rice variety CR Dhan 321 (Hindi)(2024-03) Sah, R.P.; Singh, Lalan Kumar; Anandan, A.; Meher, J.; Panda, S.; Bose, L.K.; Pandi, G.G.P.; Keerthana, U.; Annamalai, A.; Kumar, G.A.K.; Nayak, A.K.Technical ReportItem Open Access Production technology for a high yielding early transplanted rice variety CR Dhan 321 (Odia)(2024-03) Anandan, A.; Sah, R.P.; Meher, J.; Panda, S.; Bose, L.K.; Pandi, G.G.P.; Keerthana, U.; Annamalai, M.; Kumar, G.A.K.; Nayak, A.K.