Effect of Metal Nanoparticles on E. Coli Induced Pathology in Broilers
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Date
2020
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MAFSU, Nagpur
Abstract
Colibacillosis is a prevalent bacterial disease in the poultry industry
throughout the world caused by avian pathogenic Escherichia coli. The multidrug
resistance in E. coli strains makes it a potential reservoir for gene resistance possessing zoonotic health risks. To combat the antibiotic resistance alternative
techniques for diagnosis and treatment of E. coli are need at the moment. The
present research was outlined to elucidate the therapeutic potential of metal
nanoparticle mixture as an alternative for the antibiotic resistance in E. coli
infection. During the research, the revival of E. coli isolates was done and
confirmed by PCR. One of the E. coli isolates positive for both genes was used
for challenging the broilers at the concentration of 1x 108 CFU/bird by giving 1
ml inoculum through oral route.
A notable difference (P<0.01) in the efficacy of Ag nanoparticles
followed by the mixed metal nanoparticle mixture was seen in the in vitro assay
as compared with other metals viz. Mg, Cu, Fe, Se, Zn, Cr, Sn. In the present
experiment, the addition of metal nanoparticle mixture (1- 28 days) in birds
challenged with E.coli infection showed significantly (P<0.01) higher body
weight, lower feed intake and comparatively better FCR in respect with other
groups. Histopathological lesions in the lungs revealed congestion, haemorrhages,
exudates and infiltration of leucocytes. Severe pericarditis and perihepatitis were
evident. Peritonitis with distended caeca was observed. Haemorrhages in lamina
propria, disruption of villi, sloughing of mucosa along with mononuclear
infiltration of cells was found in the intestine. Nonetheless, significant (P<0.01)
reduction in intensity of the lesions and bacterial colonization in intestine of N3
fed with nanoparticles from the 10th
day of age was observed at 21st day of age.
The result of present study indicates that metal nanoparticles can be
further explored as an alternative to antibiotic growth promoters.