Browsing by Author "Nainar, A.M."
Now showing 1 - 6 of 6
Results Per Page
Sort Options
OtherItem Open Access Assessment of Multi-locus DNA Fingerprint Profiles as Genetic Tools for Analyzing Broiler Pure Lines(TANUVAS, 2000) Mishra, S.K.; Thiagarajan, V.; Nainar, A.M.; Reddy, P. Ranga; Raj, R. Kumara; Mahapatra, S.C.ArticleItem Open Access Comparison of Virus Isolation and Polymerase Chain Reaction for Diagnosis of Peste Des Petits Ruminants(Acta Virologica, 2001) Brindha, K.; Raj, G. Dhinakar; Ganesan, P.I.; Thiagarajan, V.; Nainar, A.M.; Nachimuthu, K.; TANUVASOculonasal swabs and tissue samples collected from peste des petits ruminants (PPR) suspected sheep and goats were tested for presence of the virus of peste des petits, ruminants (PPRV) or its RNA by reverse transcription-PCR (RT-PCR) and virus isolation (VI). Of 44 samples 31.8% and 40.9% were positive by VI and RT-PCR, respectively. The RT-PCR-positive samples were subjected to the nested PCR. Three of six samples positive by RT-PCR but negative by VI were negative by the nested PCR. The specificity and accuracy of the nested PCR were higher than those of the RT-PCR although the sensitivity of both tests were similar. Nucleotide sequencing of one nested PCR product revealed a 92% homology with the sequence available in the GenBank (Acc. No. Z37017).ArticleItem Open Access Development of an Enzyme Linked Immunosorbent Assay for Estimation of Buffalo Immunoglobulins Using Anti Bovine Light Chain Monoclonal Antibodies(Buffalo J., 2003) Raj, G. Dhinakar; Ratnaprabha, S.; Matheswaran, K.; Deshpande, M.; Srikumaran, S.; Nainar, A.M.; Nachimuthu, K.; TANUVASThe monoclonal antibodies (Mabs) against bovine light chain cross-reacted with buffalo immunoglobulin (Ig) in radio immune assay. These Mabs were used as capture antibodies in an enzyme linked immunosorbent assay (ELISA) to capture all buffalo Ig isotypes in serum and colostrums. The captured Igs were detected using a commercial antibovine peroxidise conjugate. This test was compared to the conventional radial immune diffusion (RID) test using purified buffalo If from colostrums and antisera raised against it. Of the 61 samples of serum and colostrums analyzed using both these tests, the range of Ig concentrations in buffalo serum using RID and ELISA were respectively 7.44 – 39.10 mg/ml and 14.67 – 73.66 mg/ml (n=47). Similar concentration in colostrums was 22.32 – 90.77 by RID and 19.83 – 94.75 mg/ml by ELISA (n=14). The correlation coefficient of these concentrations together was 0.91. The capture ELISA was simple to perform, rapid and can be used to rapidly identify buffalo calves with ‘Failure of passive transfer’ to prevent neonatal calf mortality.ArticleItem Open Access Egg: Embryo Weight Ratio as an Indicator of Dwarfism Induced by Infectious Bronchitis Virus(Avian Pathology, 2004-06) Raj, G. Dhinakar; Kumar, K. Suresh; Nainar, A.M.; Nachimuthu, K.; TANUVASA Simple objective method to quantify embryo dwarfism induced by infectious bronchitis virus in embryonated chicken eggs has been used to determine endpoints in virus titration and neutralization assays. The EE ratios were compared with the uninoculated control eggs and endpoints could be calculated objectively. EE indices were also calculated by dividing the EE ratios of inoculated embryonated chicken eggs by the mean EE ratio of uninoculated controls, or in the case of virus neutralization tests by the mean EE ratio of eggs inoculated with virus alone. Although this mean EE index did not reflect the dwarfing (or lack of it) in individual eggs, it served as a group indicator. This method would be useful to observe embryo lesions especially in field (non-egg adapted) infectious bronchitis virus isolates, which does not cause observable dwarfing until several embryo passages.ArticleItem Open Access Interaction Between Genomes of Infectious Bronchitis and Newcastle Disease Viruses Studied by Reverse Transcription-Polymerase Chain Reaction(Acta Virologica, 2004) Subramanian, B.M.; Raj, G. Dhinakar; Kumanan, K.; Nachimuthu, K.; Nainar, A.M.; TANUVASReverse transcription-polymerase chain reaction (RT-PCR) with specific primers for the SI gene of IBV and for the fusion protein cleavage site of NDV was used for detection of Infectious bronchitis virus (IBV, the family Coronaviridae) and Newcastle disease virus (NDV) genomes. The sensitivity of IBV and NDV RT-PCR was 10^3.7 and 10^3.0 EID50, respectively. Although a multiplex RT-PCR could detect and differentiate NDV and IBV genomes present in the same sample, there was a slight inhibition of the IBV PCR if a high amount of NDV genome was present in the sample. To overcome this problem a separate PCR for each virus was used to assess the interaction between vaccine IBV and NDV either inoculated singly or together into chickens. In the groups vaccinated with the Newcastle disease (ND) vaccine alone, the viral genome was detected on days 2, 4 and 7 post vaccination (p.v.), while in the chickens given the infectious bronchitis (IB) vaccine alone, the viral genome was detected only on day 4 p.v. In the group inoculated with both vaccine viruses there was a 10^3-fold reduction in the cDNA dilution factor on day 4 p.v. for both IBV and NDV genomes. This demonstrated clearly that when both these vaccines are administered there is a transient reduction in the replication of both viruses, probably due to their competition for the same target epithelial cells in the respiratory tract.ArticleItem Open Access Pathotyping of Newcastle Disease Virus Isolates from Pet Birds(Acta Virologica, 2005) Senthuran, S.; Vijayarani, K.; Kumanan, K.; Nainar, A.M.; TANUVAS