Browsing by Author "Nagalakshmi, K. Shrine"
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OtherItem Open Access Comparison of Single Intradermal Test and Gamma Interferon Assay for the Detection of Mycobacterium Avium Subsp. Paratuberculosis in Animals(TANUVAS, Chennai, 2007-08) Nagalakshmi, K. Shrine; Senthilkumar, T.M.A.; Ganesan, P.I.; Ramadass, P.ArticleItem Open Access Differential Diagnosis of Avian Mycoplasmosis(Indian Veterinary Journal, 2013-06) Nagalakshmi, K. Shrine; Senthilkumar, T.M.A.; Parthiban, M.; Ramadass, P.; TANUVASIn the present study, differential diagnosis of avian mycoplasmosis was conducted with 282 samples collected from different poultry farms in Tamil Nadu. The samples were cultured in Frey’s Mycoplasma broth and agar. When MG- and MS- specific primers were used for the detection of Mycoplasma infections, 95 samples showed positive results for MS (33.7%) and none of the samples were positive for MG by PCR assay. For differential diagnosis of respiratory infections, isolations with selective media were attempted for organisms such as E. coli, Pasteurella multocida and Haemophilus paragallinarum. PCR assay was used for detection of Haemophilus paragallinarum as well as Newcastle Disease Virus and infectious Bronchitis Virus. Pure cultures of E. coli were obtained in 184 samples out of 282 samples tested (65.2%), which were further confirmed by biochemical tests. Pasteurella multocida isolates were obtained in 169 samples out of 282 samples (59.9%) and these isolates were also confirmed by biochemical tests. The samples were also screened by PCR using specific primers and only 3 samples showed the presence of Haemophilus paragallinarum out of 282 samples tested. Of 100 samples tested by RT-PCR for detection of NDV and IBV, only seven samples were found to be positive for IBV.ThesisItem Open Access Differential Diagnosis of Avian Mycoplasmosis(TANUVAS, 2005) Nagalakshmi, K. Shrine; TANUVAS; Kumar, T.M.A. Senthil; Ramadass, P.ArticleItem Open Access Leptospirosis in a Lion(Indian Journal of Animal Sciences, 2009-04) Vijayarani, K.; Nagalakshmi, K. Shrine; Malathi, G.; Jayathangaraj, M.G.; Sathasivam, S.; Kumanan, K.; TANUVASArticleItem Open Access Simulaneous Infection of Johne's and Tuberculosis in a Cow(Indian Veterinary Journal, 2009-09) Vijayarani, K.; Nagalakshmi, K. Shrine; Balachandran, C.; Kumanan, K.; TANUVASArticleItem Open Access Simultaneous Infection of Johne's and Tuberculosis in a Cow(Indian Veterinary Journal, 2009-09) Vijayarani, K.; Nagalakshmi, K. Shrine; Balachandran, C.; Kumanan, K.; TANUVASMycobacterium avium subsp. paratuberculosis (MAP) causes Johne's disease (JD) in domestic and wild ruminants. Apart from causing JD, MAP is also suspected to be associated with Chrone;s disease in humans (Naser et al., 2002). Similarly, Mycobacterium bovis (MB) infection occurs in diverse groups of animals, which include farm animals of economic importance, wild animals and humans (Ayele et al, 2004). There are only limited reports on these two important infections in India (Kumar et al., 1998; Mishra et al., 2005; Singh et al., 2007). The simultanous infection of JD and tuberculosis in a cow is reported.ArticleItem Open Access Use of Nested PCR for the Detection of Mycobacterium Avium Subsp. Paratuberculosis in Faecal Samples(Indian Veterinary Association, 2014-01) Senthilkumar, T.M.A.; Parthiban, M.; Ganesan, P.I.; Nagalakshmi, K. Shrine; Ramadass, P.; TANUVASMycobacterium avium subsp. paratuberculosis (MAP) is the causal agent of Johne’s disease (JD), a chronic infectious enteritis of domestic and wild ruminants. Detection of MAP by PCR offers the advantage of reduced turnaround time compared with culture as well as PCR has been widely used as a diagnostic tool for detecting pathogens in sputum, stool, cerebrospinal fluids, blood and urine. Amplification of insertion element IS900 has proved to be highly sensitive and specific for detecting MAP strains (Collins et al., 1989).