Differential Diagnosis of Avian Mycoplasmosis
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Date
2013-06
Journal Title
Journal ISSN
Volume Title
Publisher
Indian Veterinary Journal
Abstract
In the present study, differential diagnosis of avian mycoplasmosis was conducted with 282 samples collected from different poultry farms in Tamil Nadu. The samples were cultured in Frey’s Mycoplasma broth and agar. When MG- and MS- specific primers were used for the detection of Mycoplasma infections, 95 samples showed positive results for MS (33.7%) and none of the samples were positive for MG by PCR assay. For differential diagnosis of respiratory infections, isolations with selective media were
attempted for organisms such as E. coli, Pasteurella multocida and Haemophilus paragallinarum. PCR assay was used for detection of Haemophilus paragallinarum as well as Newcastle Disease Virus and infectious Bronchitis Virus.
Pure cultures of E. coli were obtained in 184 samples out of 282 samples tested (65.2%), which were further confirmed by biochemical tests. Pasteurella multocida isolates were obtained in 169 samples out of 282 samples (59.9%) and these isolates were also confirmed by biochemical tests. The samples were also screened by PCR using specific primers and only 3 samples showed the presence of Haemophilus paragallinarum out of 282 samples tested. Of 100 samples tested by RT-PCR for detection of NDV and IBV, only seven samples were found to be positive for IBV.