Browsing by Author "Mammen, Lancy"
Now showing 1 - 6 of 6
Results Per Page
Sort Options
ArticleItem Open Access Anthrax vaccine 34F2 Sterne strain growth in nutrient agar and Soyabean casein digest agar(2012) Parthiban, S.; Mammen, Lancy; Kalaivani, S.R.; Sundrapandian, V.; Rahman, A. Habibur; TANUVASThe most widely used strain for anthrax vaccine production is 34F2 Sterne strain. The aim of the study was to determine a suitable solid media for the cultivation of 34F2 Sterne strain for large scale vaccine production. Seed material (34F2 Sterne strain) was cultured in 70 Roux flasks each containing Nutrient agar and Soyabean casein digest agar separately and allowed for sporulation. The spores were washed with sterile saline and the pure spore suspensions were pooled and weighed separately. The weight of the spore suspension was 1.801 kg and 1.830 kg for Nutrient agar and Soyabean casein digest agar respectively. The number of live spore was 1:250 in 1 ml of 10^-7 dilution for both Nutrient agar and Soyabean casein digest agar grown spore suspensions. The spore weight and spore count are almost equal for the both the solid media but based on cost and quantity of medium required the Nutrient agar is preferred over Soyabean casein digest agar.ArticleItem Open Access Enhancement Of Epsilon Toxin Titer in Alum Precipitated Enterotoxemia Vaccine(2013-12) Kishorekumar, R.; Saravanan, S.; Parthiban, S.; Mammen, Lancy; Koruth, Johnsi; Prabhakaran, V.; TANUVASEnterotoxemia, a disease that affects domestic ruminants especially sheep and goats, is caused mainly by the epsilon toxin from Clostridium perfringens type D. Its eradication is virtually impossible, control and prophylaxis are based on systematic vaccination of herds with alum precipitated whole cell with epsilon toxoid vaccines is the only efficient way in inducing protective antibody production.ThesisItem Restricted GENOME BASED STUDIES ON PARVOVIRUSES AFFECTING DOGS AND CATS(2018) Mammen, Lancy; Parthiban, M; Shoba, K; Ramesh, S; Senthilkumar, TMA; TANUVASThe emergence of Canine Parvovirus and Feline panleukopenia virus in canine and feline population has prompted inputs particularly in the areas of diagnosis, as infection rates have been found to increase in the recent past globally and in India and early detection of CPV and FPV during such periods is very important to initiate appropriate diagnostic and prophylactic measures. Vaccines against CPV and FPV has at times resulted in vaccination failure due to introduction of newer antigenic variants.ArticleItem Open Access Molecular methods for identification of antigenic types of canine parvovirus(2018) Mammen, Lancy; Parthiban, M; Shoba, K, et al.,; TANUVASCanine parvovirus and Feline panleukopenia virus are pathogens which cause acute haemorrhagic enteritis and myocarditis and is a contagious disease with high morbidity and mortality. In this study, a total of 90 faecal samples from live dogs, and tissue samples from 16 dead dogs showing symptoms of CPV and 7 samples from dead cats reported with severe clinical symptoms of FPLV were subjected to Haemagglutination assay, Conventional and Differential PCR. Our results indicates the evidence of CPV-2a and CPV-2b variants circulating among dogs population in Tamil Nadu.ThesisItem Open Access Persistence, Distribution and Behavioural Pattern of Lasota Vaccine Virus in Poultry Population(TANUVAS, 1997) Mammen, Lancy; TANUVAS; Chandran, N. Daniel Joy; Paul, W. Manohar; Pandiyan, VArticleItem Open Access Tissue distribution of the antigenic variants of canine parvovirus in various organs using nested PCR(Akinik Publication, 2018-03) Mammen, Lancy; Parthiban, M; Shoba, K; Senthilkumar, TMA; Ramesh, S; Vijayarani, K; TANUVASCanine parvovirus-2(CPV-2) is a highly contagious and fatal disease of dogs causing acute haemorrhagic enteritis and myocarditis. In this study, tissue samples such as brain, cerebellum, cerebral bulb, tonsil, retropharyngeal lymph node, thymus, lungs, myocardium, bone-marrow, liver, spleen, pancreas, kidney, bladder, mesenteric lymph node, stomach, duodenum, jejunum, colon and rectum collected from 3-9 months old dead dogs reported with severe clinical symptoms of CPV were subjected to conventional and nested PCR assay, CPV-DNA was detected in all tissues including brain tissue.