Browsing by Author "Legadevi, R."
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ArticleItem Open Access Gross, Histopathology and Molecular diagnosis of oesophagostomosis in Sheep(2018) Satish, A.C.; Nagarajan, K.; Balachandran, C.; Soundararajan, C.; Legadevi, R.; TANUVASA total of 120 tissue samples (Rumen, reticulum, omasum, abomasum, small intestine and large intestine) were collected from slaughter houses in Chennai, Kanchipuram and Tiruvallur districts and five samples from necropsy room at Department of Veterinary Pathology, Madras Veterinary College, Chennai, Tamil Nadu. Overall prevalence of GI parasites was 52.00%. Among the positive samples, single infection was found to be more (53.84%) than themixed infection (46.15%). Out of which, oesophagostomosis was 17.00% and found as single infection. In oesophagostomosis, significant gross lesions observed in the small and large intestine were multiple small to large, round to irregular, hard, fibrotic, raised nodules. The wall of the large intestine was greatly thickened and oedematous and the nodules were seen projecting into the lumen and mucosal surface was intact. On histopathological examination, ileum of sheep showed parasitic nodules with central baso-eosinophilic necrotic area, larval stages in the necrotic area, surrounded by layers of inflammatory cells and finally encapsulated in a thick fibrous connective tissue capsule. Immunohistochemistry (IHC) with Vimentin showed intense cytoplasmic positive signals DAB Brown. PCR was carried out targeting ITS2 regions of the O. columbianum and O. venulosum. DNA isolated from both the faecal eggs and adult worms showed good amplification.ArticleItem Open Access Isolation and Identification of Bacteria of Normal Flora in Handfed Exotic Psittacine Birds at Tamil Nadu, India(TANUVAS, Chennai, 2019-03) Legadevi, R.; Dillibabu, V.; Karunakaran, N.; Nagarajan, K.; Soundararajan, C.; TANUVASBacterial normal flora were identified in handfed psittacine birds (lory, amazons, lorikeet, cockatoo. conure, grey parrot, macaw, eclectus and African love birds) at Cavin estate exotic bird farm, Chennai. Tamil Nadu. A total of 54 cloacal swabs were collected from different species of psittasine birds for bacteriological screening to identify the microorganisms by morphological and biochemical characteristics.ArticleItem Open Access Isolation of Bacteria From Dead - In - Shell Chick Embryos of Psittacines(TANUVAS, Chennai, 2019-03) Dillibabu, V.; Legadevi, R.; Karunakaran, N.; Nagarajan, K.; Tamilmani, S.; Kaushika, S.; Jayakeerthil, S.; Soundararajan, C.; TANUVASThe objective of the present study is to explore the bacteria associated with the dead- in-shell eggs of psittacines. A total of 132 dead-in-shell eggs of different psittacine breeds (Macaws, Lories, Conures. Amazons, Eclectus, Cockatoos, Grey parrots and Jardine parrots) were obtained from the incubation unit of Cavin estate exotic bird farm, Chennai, Tamil Nadu and subjected to microbological analysis. The overall prevalence of bacteria isolated from the dead-in-shell was 80.30% (l06/ 132). Among these, Gram-negative bacteria were predominant (49.24%, 65/132) was observed followed by Gram-positive organisms (28.78%, 38/132) and Gram-positive filamentous organisms (2.27%, 3/132) were also isolated from dead in shell embryos.