Browsing by Author "LOHITH KUMAR. T"
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ThesisItem Open Access ISOLATION AND CHARACTERIZATION OF LISTERIA SPECIES FROM POULTRY MEAT(Anand Agricultural University, Anand, 2009) LOHITH KUMAR. T; Dr. M. N. BrahmbhattThe present study is undertaken with intension of isolating and identifying the Listeria spp. with special reference to L. monocytogenes from raw poultry meat sold at retail meat shops in Anand, Gujarat. The samples of raw poultry meat consist different edible parts of chicken such as breast, leg, liver, gizzard and wing (35 each) collected aseptically from local meat market and subjected to two stage enrichment process with UVM 1 and UVM 2 for two days each, followed by plating on three selective media viz. DRIA, PALCAM and Oxford media. The colonies showing typical colony characteristics were further characterized on the basis of their morphological and biochemical characteristics. Cultures identified as Listeria spp. were further subjected to in vitro pathogenicity (hemolysis on sheep blood agar and CAMP test), detection of different virulence associated genes and sensitivity of Listeria spp. to various antibiotics commonly used in human and veterinary treatment