Browsing by Author "Kavitha, KT"
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ArticleItem Open Access Characterization of Excretory and Secretory Larval Antigen of Toxocara canis by Western Blotting(Excellent Publishers, 2019) Kavitha, KT; Sreekumar, C; Latha, BR; Mangala Gowri, A; Nagarajan, BToxocara canis is a dog helminth which causes visceral larva migrans (VLM) in humans. The identification of specific antigens of T. canis is important in order to develop better diagnostic techniques. The excretory-secretory larval antigens of T. canis (ESLA) were prepared by in vitro culturing of T. canis larvae in RPMI 1640 medium. These antigens were separated using sodium dodecyl sulphate-poly acrylamide gel electrophoresis (SDSPAGE) which revealed 9 protein bands at a molecular weight of 17, 18, 22, 24, 26, 28, 30, 32 and 120 kDa. The immuno reactivity of excretory-secretory larval antigens of T. canis was checked by Western blotting using hyper immune serum raised in rabbits against ESLA antigen which showed six immuno reactive bands at a molecular weight of 17, 18, 22, 24, 30 and 32 kDa. These antigens merit further evaluation as candidate for use in diagnosis of toxocariasis in humans and adult dogs.ArticleItem Open Access Chewing lice (Phthiraptera) of pigeons (Columba livia) in Chennai, Tamil Nadu(2021-12) Kavitha, KT; Bino Sundar, ST; Ravi Latha, Bhaskaran; TANUVASThe purpose of this study was to document the finding of chewing lice infesting pigeons in Chennai, Tamil Nadu. A total of 18 pigeons were examined for the presence of lice, out of which five (27.8%) showed severe infestation. The infested pigeons showed restlessness, weak body condition, pruritus, skin reddening, dermatitis, anaemia and lesions on their feathers. A total number of 230 lice were collected from the infested pigeons. Three species of chewing lice, Colpocephalum turbinatum (n=149), Columbicola columbae (n=70), and Campanulotes compar (n=11) were identified morphologically. It was observed that C. turbinatum (64.8%) was the most common parasite, followed by C. columbae (30.4%). C. compar (4.8%) showed the lowest rate of infestation.ArticleItem Open Access Entomopathogenic Fungi Prevalent in Soil and Rhipicephalus (B) microplus Ticks in Tamil Nadu(2020-07) Kavitha, KT; Saranya, D; Ravi Latha, Bhaskaran; TANUVASA study to determine the best methods to isolate entomopathogenic fungal species from fieldcollected samples of soil and ticks at different regions of Tamil Nadu was conducted. The isolation was carried out by soil plating method with selective media and insect bait method with Rhipicephalus (B) microplus ticks. A total of 12 isolates of entomopathogenic fungi were isolated from tick as well as soil samples. The most predominant species was Beauveria bassiana (7 isolates) followed by Metarhizium anisopliae (4 isolates) and Isaria fumosorosea (1 isolate). The bait method with ticks isolated 14.5 per cent while the soil plating method it was 8.8 per cent. The other colonies of fungi included Aspergillus flavus, A. niger, Mucor, Rhizopus and Fusarium sp. The higher incidence of entomopathogenic fungi was observed using the bait method with high frequencies of B. bassiana and M. anisopliae. The bait method with R. (B) microplus ticks was a sensitive method for the isolation of entomopathogenic fungi in soil.ArticleItem Open Access IMMUNOLOCALISATION OF MIDGUT ANTIGEN OF RHIPICEPHALUS HAEMAPHYSALOIDES BY INDIRECT IMMUNOPEROXIDASE TEST USING METHYLENE GREEN COUNTER STAIN(TANUVAS, 2011-03) Kavitha, KT; Latha, Bhaskaran Ravi; Raj, G Dhinakar; John, Lalitha; TANUVASImmunolocalisation of midgut antigen of Rhipicephalus haemaphysaloides was performed by indirect immunoperoxidase test using methylene green as counter stain. Semi engorged adult female ticks were fixed in bouins fixative, embedded in paraffin wax and sectioned. The tick sections were treated with sera from immunized animals showed positive brown colour reaction on the entire surface of digestive cells and midgut epithelial membrane. The methylene green was used for counter staining which clearly differentiated the midgut from other tick tissues.ArticleItem Open Access Relationship between infective dose of larvae on pathology and antibody response in experimental toxocarosis in mice(2019-12) Kavitha, KT; Sreekumar, C; Latha, BR; Mangala Gowri, A; et al.; TANUVASToxocara canis, a cosmopolitan parasite of canines and the major causative agent of human toxocariasis, constitutes a serious epidemiologic problem in many countries. In the present study, the dynamics of IgG antibody production, larval distribution and pathological changes were evaluated after experimental infection of BALB/c mice with two doses of embryonated eggs of T. canis. The mice were divided into 3 groups; Group I, infected with 100 eggs; Group II, infected with 1000 eggs and Group III, uninfected control mice. The mice were bled at 0, 7, 14, 28, 45, 60, 90, 120 and 150 days post infection (dpi). Indirect ELISA was performed for detecting IgG antibody response. On each date, one mouse from each group was sacrificed for larval recovery from lung, liver, brain, eye and musculature and for histopathology. The IgG antibodies in both the infected groups were detected from 7 dpi which increased gradually and peak at 60 dpi. After, 90 dpi, the IgG antibody response began to decline but it was detected until the end of the experiment. The IgG antibody response of group II mice was significantly higher than the group I mice throughout the observation period. Statistically highly significant difference was observed between the infected groups (P<0.01) from 7-150 dpi. These results showed that the magnitude of the anti-Toxocara antibody response was directly proportional to the dose of the inocula. In the lung and liver, larvae were found in both the infected groups from 7 to 21 dpi where as in the brain and musculature, these were recovered in both the groups from 7 to 150 dpi. The group inoculated with 1000 eggs showed a greater number of larvae in the brain. It was found that the degree of larval recovery was dependent on the size of the infecting dose. Histopathological examination revealed inflammatory infiltrate and haemorrhages in the lung and liver parenchyma from 7 to 60 dpi of both the infected groups except brain. Granulomas were seen around the larvae in the section of lung and liver from 28 to 60 dpi. However, in the section of brain, the larvae were seen from 14 to 150 dpi but in the eye, the larva was seen from 90 and 120 dpi without inflammatory reaction surrounding the larvae.ArticleItem Open Access ஆடுகளில் குடற்புழு நீக்க மருந்தின் எதிர்ப்புத் தன்மையை எதிர்கொளல் - ஒரு புதிய அணுகுமுறை(TANUVAS, 2021-03) Kavitha, KT; Sreekumar, C; Ravi Latha, Baskaran; TANUVAS