Browsing by Author "Heena Rani"
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ThesisItem Open Access An analytical study of community radio stations of Haryana(CCSHAU, 2012) Heena Rani; Sethi, NishiCommunity Radio is a type of radio service that offers a third model of radio broadcasting beyond commercial and public service. Thus an analytical study of CRS of Haryana was conducted. The locale of the study was Haryana state. From Haryana state all the 5 CRS were selected and programmes of a month i.e. October, 2011 constituted the sample size. Existing status of CRS of Haryana and their programmes were analysed separately through content analysis. CRS, Chaudhary Charan Singh Haryana Agricultural University of Hisar district was selected to study the perception of programme on rural listeners. Dabra and Satroad Khurd villages were purposively selected. A total sample of 100 CR listeners i.e. 50 from each village were selected through snowball technique. Different socio- personal, economic, psychological and communication variables were selected as independent variables whereas perception of listeners was selected as dependent variable. A well structured interview schedules were constructed for collecting information in view the objectives of the study. Percent time, percent PQP percent mean score index, percentages, mean score and ranks were calculated for interpretation of data. The findings revealed that all the CRS were started between January, 2009 to July, 2010. A lot of variation was observed in broadcast time ranging between 4 hours to 22 hours. CRS, Hello Sirsa, CCS HAU, Hisar, Radio Manav Rachna, Faridabad were run by educational institutes. Most of the CRS were adopting feedback mechanism through phone in programmes and were inviting members of local communities for their programmes. CRS, Radio Manav Rachna, Faridabad got 1st rank for most well equipped CRS of Haryana Maximum outdoor shootings with community were conducted by CRS, Radio Mewat. CCS HAU, Hisar and Radio Mewat CRS’s emphasis was on information and educational programmes, (69.47% and 48% respectively) and majority of the listeners (46%) were utilizing high level of localite information sources but low level of cosmopolite information sources (49%) and mass media exposure (53%). Maximum utility in terms of gain in knowledge was perceived from programmes related to agriculture and animal science and veterinary. Regarding perception of listeners programme on agriculture got the highest rank and PQP value 80.7 per cent in overall perceptions about quality of programme. ‘CRS should be built up in the villages’ was the main implication suggested by all 5 CRS. Man power, administrative and technical limitations were found major limitations. The major implication suggested by the CR listeners was the ‘Range with good audio quality of the CR should be increased’. ‘Duration of broadcast programmes is not sufficient’ was main limitation by the CR listeners of CCS HAU, Hisar CRS.ThesisItem Restricted Expression patterns of glucosinolate transporter genes GTR1 and GTR2 in relation to variation patterns for leaf and seed glucosinolates in Brassica juncea(Punjab Agricultural University, Ludhiana, 2018) Heena Rani; Sharma, SanjulaThe present study was aimed at relating expression patterns of glucosinolates transporter genes (GTR1 and GTR2) with varied pattern of glucosinolate (GLS) content at different developmental stages in mustard (B. juncea). A total of two hundred genotypes were screened for their leaf and seed GLS content and were categorized into two sets. One set with GLS genotypes containing high GLS content (3.29 to 23.18 μmoles/g DW in leaves and 68.17 to 116.38 μmoles/g defatted meal in seeds), and the another set with genotypes containing low GLS content (2.42 to 8.64 μmoles/g in leaves and 24.42 to 30.14 μmoles/g defatted meal in seeds). A huge difference was observed between leaves and seed GLS content indicating the involvement of some other plant tissue as major site for GLS biosynthesis and the information was uncovered by analyzing GLS content in tissues from various developmental stages for GLS content. GLS content increased from open buds (22.02-24.91 μmoles/g DW in high GLS set; 11.17-19.06 μmoles/g DW in low GLS set) through wilted flowers (23.8-35.72 μmoles/g DW in high GLS set; 16.3-21.3 μmoles/g DW in low GLS set) to premature siliques at 14 DAF (43.54-69.14 μmoles/g DW in high GLS set and 21.9-27.68 μmoles/g DW in low GLS set). However, followed by a decrease from 14 DAF through 21 DAF siliques (25.28-42.69 μmoles/g DW in high GLS set; 17.63-25.09 μmoles/g DW in low GLS set) to 30 DAF siliques (9.59-32.68 μmoles/g DW in high GLS set; 9.01-14.7 μmoles/g DW in low GLS set) which indicates the transport of GLS occurs from silique walls towards developing seeds. The results were confirmed by the qRT-PCR gene expression studies where fold change in gene expression of GTR1 and GTR2 increased from open buds (almost negligible) to siliques at 30 DAF (1.93-12.81 fold for GTR1 and 0.38-3.60 fold for GTR2) which indicate an increase in GLS transport as a plant progresses towards maturity. GLS profiling results showed sinigrin as the most abundant GLS in leaves, whereas, gluconapin was highest in seeds. Removal of these anti-nutritional compounds (GLSs) will improve the mustard meal quality and allow its usage as food and feed purpose that could help in eradicating malnourishment problem from the country. Our study could prove helpful in lowering the GLS content by identifying alternate alleles that prevent accumulation of GLSs in developing seeds.ThesisItem Restricted Malt quality and thermostability studies in mutants, hulled, hull-less and wild genotypes of barley (Hordeum vulgare L.)(Punjab Agricultural University, 2023) Heena Rani; Bhardwaj, Rachana DDespite various attempts to develop malt hydrolytic enzymes, the catalytic activity and thermostability of these enzymes are still far from sufficiency for their use in the malting process. The present study explored diverse barley germplasm to identify the genotypes with high activity and thermostability of enzymes alongwith the proteins responsible for high malt yield. Based on enzymatic assays, we selected BL1714T, DWRB137T and BL2105T with highly activity and thermostability of α and β amylases and limit dextrinase, which are key diastatic power enzymes. The barley proteome of these genotypes was analyzed before and after kilning using label-free quantitative global proteomic approach against the control genotypes; BL1718Ctrl, DWRUB64Ctrl and BL2083Ctrl, respectively. Among various isoforms of hydrolytic enzymes Bamy 1 and Amy1_2 were more abundant in thermostable genotypes indicating their role in controlling the activity of these enzymes at high temperatures. Gene ontology enrichment analysis indicated disulfide reductase, exopeptidase, serine-type peptidase (serpin), cell wall hydrolase and oxidoreductase activities as most common enriched terms in thermostable genotypes. These proteins work to release the bound form of hydrolytic enzymes, resulting in their increased overall activity. These proteins could be employed as biochemical indicators for identifying barley lines with high activity and thermostability. Using GC-MS/MS, we identified maltose, glucose, talose, sucrose, fructose, turanose and other minor sugars in green and kilned malt. Talose and turanose were identified among major sugars for the first time during malting. Further, malt quality analysis suggested that βglucanase and malt β-glucan can be used as additional traits to produce malt with superior quality.