Browsing by Author "Gomathy, VS"
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ThesisItem Open Access INFLUENCE OF EARTHWORM MEAL SUPPLEMENTATION ON INTESTINAL PHYSIOLOGY AND GROWTH PERFORMANCE IN JAPANESE QUAILS(TANUVAS, 2016) Deepika, B; Leela, V; TANUVAS; Gomathy, VS; Ezhilvalavan, SThe biological experiment was carried out in 240 numbers of clay old Japanese quails (Coturnix coturnix japonica) to evaluate the influence of earthworm meal supplementation on intestinal physiology, haemato-biochemical changes, growth performance and morphological changes in small intestine. The quail chicks were fed ml Iibitum upto 6 Weeks of age with four different equi-nitrogenous and equi-caloric diets formulated by replacing dietary fish meal partially by earthworm meal. in four diets Gl (0), G2 (50), G3 (75) and G4 (100) per cent earthworm meal as replacement for fish meal.ThesisItem Open Access INFLUENCE OF HEPARIN BINDING PROTEIN ON IN VITRO SPERM CHARACTERISTICS OF FROZEN BUFFALO SEMEN(TANUVAS, 2016) Kikon, Yanben M; Loganathasamy, K; TANUVAS; Gomathy, VS; Vijaya Rani, KThe present study was conducted to assess the effect of exogenous supplementation of heparin binding protein (HBP) on sperm motility, viability, morphology, functional membrane integrity, acrosomal integrity, mitochondrial membrane potential (MMP), DNA integrity and lipid peroxidation status (LPO) of frozen buffalo semen. Buffalo semen straws from 10 bulls were procured from Central Frozen Semen Production and Training Institute, Hesseraghatta. Banglore- 560088.ArticleItem Open Access Osteopontin ameliorates sodium nitroprusside induced free radical damage on sperm motility of frozen thawed buffalo semen(2020) Viswam, Visakh; Loganathasamy, K; Gomathy, VS; Reena, D; TANUVASThe experiment was undertaken to study sodium nitroprusside (SNP) induced free radical damage on sperm motility of frozen thawed buffalo semen and the ameliorative effects of osteopontin (OPN) supplementation on sperm motility. Buffalo frozen semen straws from 8 ejaculates of 6 bulls were procured from Central Frozen Semen Production and Training Institute, Hessarghatta, Banglore and stored at Semen Bank, Madras Veterinary College, Chennai. The semen straws were thawed and seminal plasma and semen extender were removed from spermatozoa by centrifugation. Spermatozoa were suspended in 1mL capacitation medium (control), with the addition of 100μg/mL of OPN (treatment I) or 100μM/mL of SNP (treatment II) or 100μg/mL of OPN + 100μM/mL of SNP (treatment III). Semen with capacitation medium alone without any supplementation served as control. The contents were incubated at 37ºC for 4 h and the post capacitation sperm motility was observed under bright field microscopy. The post capacitation sperm motility was significantly (P<0.05) higher in treatment I (71.80% ± 0.04) as compared to control (61.71% ± 0.03), treatment II (24.82% ± 0.07) and treatment III (46.64% ± 0.05). But, the post capacitation motility in treatment II and III were significantly (P<0.05) lower than control. The post capacitation motility in treatment III was significantly (P<0.05) higher than treatment II. The study indicated that addition of SNP alone in the capacitation medium has detrimental effects on the sperm motility and addition of OPN alone in the capacitation medium exerts beneficial effects on sperm motility. When both OPN and SNP were added in the capacitation medium, OPN partially ameliorated the toxic effects of SNP on the sperm motility of frozen thawed buffalo semen.ArticleItem Open Access Sperm Morphology and DNA Integrity of Frozen Thawed Buffalo Semen Treated with Heparin Binding Protein(2020-07) Kikon, Yanben M.; Loganathasamy, K; Gomathy, VS; Vijayarani, K; TANUVASThe present study was conducted to assess the sperm morphology and DNA integrity of frozen thawed buffalo semen treated with heparin binding protein (HBP). Buffalo semen straws from 10 bulls were procured from Central Frozen Semen Production and Training Institute, Hesseraghatta, Banglore-560088. The frozen straws were thawed at 37ºC for 30 seconds and emptied into a 15mL sterile plastic centrifuge tube containing 1mL capacitation medium (control), addition of 25μg/mL (treatment I), 50μg/mL (treatment II) and 100μg/mL (treatment III) of HBP. The contents were incubated at 37ºC for 2 hours. After incubation, the sperm morphology was determined by Rose Bengal stain technique. Morphologically normal sperm in control, HBP treatment I, II and III were 86.70% ± 1.52, 87.00% ± 1.21, 87.00% ± 1.28 and 86.55% ± 1.59 respectively. There was no significant difference between control and HBP treatments with respect to sperm morphology. The sperm DNA integrity was assessed by acridine orange stain method. There was no significant difference among control (90.15% ± 1.14), HBP treatment I (90.25% ± 1.25), II (89.85% ± 1.05) and III (90.40% ± 1.16) with respect to sperm DNA integrity. This study indicated that HBP supplementation in capacitation medium did not alter the sperm morphology and DNA integrity.