Sperm Morphology and DNA Integrity of Frozen Thawed Buffalo Semen Treated with Heparin Binding Protein
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Date
2020-07
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Abstract
The present study was conducted to assess the sperm morphology and DNA integrity of
frozen thawed buffalo semen treated with heparin binding protein (HBP). Buffalo semen
straws from 10 bulls were procured from Central Frozen Semen Production and Training
Institute, Hesseraghatta, Banglore-560088. The frozen straws were thawed at 37ºC for 30
seconds and emptied into a 15mL sterile plastic centrifuge tube containing 1mL
capacitation medium (control), addition of 25μg/mL (treatment I), 50μg/mL (treatment II)
and 100μg/mL (treatment III) of HBP. The contents were incubated at 37ºC for 2 hours.
After incubation, the sperm morphology was determined by Rose Bengal stain technique.
Morphologically normal sperm in control, HBP treatment I, II and III were 86.70% ± 1.52,
87.00% ± 1.21, 87.00% ± 1.28 and 86.55% ± 1.59 respectively. There was no significant
difference between control and HBP treatments with respect to sperm morphology. The
sperm DNA integrity was assessed by acridine orange stain method. There was no
significant difference among control (90.15% ± 1.14), HBP treatment I (90.25% ± 1.25), II
(89.85% ± 1.05) and III (90.40% ± 1.16) with respect to sperm DNA integrity. This study
indicated that HBP supplementation in capacitation medium did not alter the sperm
morphology and DNA integrity.
Description
TNV_IJCMAS_2020_9(7)3572-3579
Keywords
Veterinary Science