Browsing by Author "Borkataki, Sanku"
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ThesisItem Open Access Antimicrobial properties of Chrysomyia megacephala larvae in chronic wound healing(Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu (J&K), 2019-11-26) Tariq, Mufeeda; Borkataki, SankuThe present study was conducted to elucidate the effectiveness of antimicrobial properties of Chrysomyia megacephala larvae in healing of contaminated excisional wound in Wistar rats. A total of 36 male Wistar rats were taken for the study. Wounds were created on all and induced with pure colonies of bacteria Staphylococcus aureus. The animals were then divided into three groups according to the treatment given i.e. Control (C), Antibiotic (A) and Maggot (M) treated groups with 12 animals each. Colonization of larvae to be used for medicinal purpose was done on three different diets i.e. sheep liver, fish meat and liver extract. The variable wound kinetic parameters revealed significant reduction in wound area with maximum contraction (90%), early elimination of bacterial bioburden (3.33±0.02) and significant reduction in wound pH (6.14±0.09) in two weeks in maggot treated group when compared to control and antibiotic treated groups. Colonization of larvae of Chrysomyia megacephala in three different diets showed total duration of life cycle on fish meat was shorter (240.88±28.09h) as compared to sheep liver(295.63±13.82h) and liver extract (557.38±11.7h) at the temperature of 27±2o C and 70% RH. Biochemical analysis of excretions and secretions (ES) of L1 larvae were found to be alkaline with the mean pH values of 8.83±0.17 and total protein was found to be 1.54±0.16mg/ml. Protein profiling of the same was done by SDS-PAGE which revealed several polypeptides of different molecular weights,among which polypeptides of 25 and 35 kDa were more prominent. In vitro antimicrobial activity of ES was tested using optical density method and showed its antimicrobial activity against S. aureus.ThesisItem Open Access EVALUATION OF MAGGOT THERAPY IN THE TREATMENT OF CHRONIC WOUND IN RAT(Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, 2017) Borkataki, Sanku; Katoch, RajeshThe present study was aimed to evaluate the effectiveness of maggot therapy in healing of chronic wound in non-diabetic and Streptozotocin induced diabetic Wistar rat. A total 96 male Wistar rats were separated in to two equal halves as non-diabetic and diabetic groups. Wounds were created in all and inoculated with mixed colonies of bacteria namely Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Post inoculation each group was further divided in 4 groups with 12 rats in each being represented as control, antibiotic, maggot and maggot and antibiotic treated. All treatments were applied once and held for 24 hours. Different wound kinetics in maggot treated wounds revealed significant reduction in wound area with maximum contraction, early elimination of bacterial bioburden, significant reduction of wound pH and increase in Hb level in comparison to antibiotic and control group respectively (P<0.05). Oxidative stress parameters revealed significant increase of Glutathione-S-transferase with a decreased level of Lipid peroxidation in maggot treated wounds in comparison with other groups (P<0.05). The formation of collagen tissue was also evident by higher concentration of hydroxyproline in granulation tissues in maggot treated groups. The histopathological examination of wounded tissue of maggot treated groups showed early and better epithelialization, collagenation and neovascularization with complete healing of wound in non-diabetic rat in two weeks and in diabetic rat in three weeks in comparison to antibiotic and control group, respectively. The effect of maggot and maggot with antibiotic combination, used in the present study, didn’t reveal any significant difference in healing of wound. Colonization of Lucilia sericata larvae in three different diets in room temperature showed total duration of life-cycle on red meat of sheep was shorter (12.29±0.16 days) where as in artificial diets i.e. blood agar and nutrient agar were (14.41±0.21) and (17.55±0.19) days, respectively.