Antimicrobial properties of Chrysomyia megacephala larvae in chronic wound healing
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Date
2019-11-26
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Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu (J&K)
Abstract
The present study was conducted to elucidate the effectiveness of antimicrobial properties of Chrysomyia megacephala larvae in healing of contaminated excisional wound in Wistar rats. A total of 36 male Wistar rats were taken for the study. Wounds were created on all and induced with pure colonies of bacteria Staphylococcus aureus. The animals were then divided into three groups according to the treatment given i.e. Control (C), Antibiotic (A) and Maggot (M) treated groups with 12 animals each. Colonization of larvae to be used for medicinal purpose was done on three different diets i.e. sheep liver, fish meat and liver extract. The variable wound kinetic parameters revealed significant reduction in wound area with maximum contraction (90%), early elimination of bacterial bioburden (3.33±0.02) and significant reduction in wound pH (6.14±0.09) in two weeks in maggot treated group when compared to control and antibiotic treated groups. Colonization of larvae of Chrysomyia megacephala in three different diets showed total duration of life cycle on fish meat was shorter (240.88±28.09h) as compared to sheep liver(295.63±13.82h) and liver extract (557.38±11.7h) at the temperature of 27±2o C and 70% RH. Biochemical analysis of excretions and secretions (ES) of L1 larvae were found to be alkaline with the mean pH values of 8.83±0.17 and total protein was found to be 1.54±0.16mg/ml. Protein profiling of the same was done by SDS-PAGE which revealed several polypeptides of different molecular weights,among which polypeptides of 25 and 35 kDa were more prominent. In vitro antimicrobial activity of ES was tested using optical density method and showed its antimicrobial activity against S. aureus.
Description
Antimicrobial properties of Chrysomyia megacephala larvae in chronic wound healing
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