STUDY ON COMPARATIVE FIELD EFFICACY OF AAU DEVELOPED MICROBIAL FORMULATIONS AGAINST Meloidogyne incognita CAUSING ROOT-KNOT DISEASE OF OKRA.

Abstract
Pot experiment was carried out during Kharif 2022 in the Net House of Department of Nematology, AAU, Jorhat-13 to evaluate the efficacy of five AAU developed bioformulations viz.,Bio-Veer, Bio-Monas, Biofor-Pf2, Biogreen and AAU Bioguard against Meloidogyne incognita in okra under Net house condition. 2000 cc capacity pots were filled with sterilized pot mixture of soil: sand : clay @ 2:1:1. Bioformulations were applied in the pots as per the treatments viz., T1 : Bio-Veer @20g/kg soil; T 2 : Bio-Monas @ 20g/kg soil; T 3 : Biofor- Pf2 @ 20g/kg soil; T4 : Biogreen @ 20g/kg soil; T5: AAU Bioguard @ 20g/kg soil one week prior to sowing the seeds. One untreated control was kept for comparison. Three to four leaved okra seedlings were inoculated with freshly hatched second stage juvenile of M. incognita 1J2/g of soil. The experiment was terminated at 60 days after sowing. It was revealed that all the AAU developed bio-formulations significantly increased the growth of okra and reduced the nematode infestation. However, Biogreen exhibited the best result in increasing the plant growth parameters and reducing the nematode infestation, followed by and Biofor-Pf2. Two promising AAU developed bio-formulations (Biogreen and Biofor-Pf2) were tested under field condition in a naturally infested field of M. incognita in okra during Kharif 2023. The treatments were: T1 : Application of Biogreen @ 1.5 tons/ha; T2 : Application of Biogreen @2 tons /ha; T3 : Application of Biofor Pf2 @ 1.5 tons/ha; T4 : Application of Biofor Pf2 @ 2 tons/ha; T5 : Application of decomposed cowdung @ 2 tons/ha; T6 : Chemical control (Fluensulfone 2% Gr @ 1 kg ai/ha) T7 : Control. The experiment was conducted in 3mx4m size plots following RBD. The result revealed that both the bio-formulation was effective in increasing plant growth parameters of okra and reducing the infestation of M. incognita. Amongst the bio-formulations, application of Biogreen @ 2t/ha gave the best result in increasing plant growth parameters of okra and reducing the infestation of M. incognita followed by application of Biogreen @ 1.5 t/ha.
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