EXPRESSION OF GONADOTROPINS AND THEIR RECEPTORS IN ATTAPPADY BLACK AND MALABARI GOATS

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dc.contributor.advisorK. Raji
dc.contributor.authorBHUVANA PLAKKOT
dc.date.accessioned2022-10-29T09:30:10Z
dc.date.available2022-10-29T09:30:10Z
dc.date.issued2019-12-31
dc.descriptionThesis Submitted in partial fulfilment of the requirement for the degree of Master of veterinary Science in Veterinary Physiologyen_US
dc.description.abstractThe difference in prolificacy between high prolific Malabari and low prolific Attappady Black goat breeds of Kerala has been evaluated by gonadotropins and their receptors gene expression studies, and analysis of steroid hormones. Six numbers of experimental animals, each from Malabari and Attappady Black breeds were selected for the study from the culling list of University Sheep and Goat Farm, Kerala Veterinary and Animal Sciences University, Mannuthy. Ovaries, pituitary and serum samples were collected from animals slaughtered at the proestrus stage of the oestrus cycle. By radioimmunoassay technique, a significant difference was observed in serum oestradiol concentrations, with Malabari having a higher mean (44.22±7.00 pg/mL) when compared to the Attappady Black breed (13.98±4.35pg/mL). The ovarian antral follicles were isolated and classified into small (1-3 mm) and large (>3 mm) follicles. RNA isolated from the follicles and pituitaries were used for expression studies. By performing quantitative real-time PCR technique, it was observed that between the breeds, there was no significant difference in the level of FSHβ expression in the pituitary, FSHR expression and LHR expression in follicles. However, there was a significant difference in the pituitary LHβ expression between breeds, with a significantly (P<0.01) high level in Attappady Black compared to Malabari breed. A non-significant decrease in (fold-change of 0.64) FSHR expression and a non-significant increase in (fold-change of 1.4) LHR expression were observed from small to large follicles in goats. Irrespective of breeds, a non-significant negative correlation (-0.49) was observed between oestradiol and FSHβ expression. Also, a non-significant negative correlation (-0.15) was observed between LHβ expression and progesterone concentration. A positive correlation although not very significant was found between FSHβ expression and FSHR expression in goats irrespective of breeds. Similarly, between FSHβ expression and LHR expression in goat antral follicles, a significant positive (P<0.05) correlation was observed. The antral follicle FSHR expression irrespective of breeds, showed a significant (P<0.05) positive correlation (0.46) with the oestradiol concentration. No correlation was found between pituitary overall LHβ and follicle LHR expression in antral follicles irrespective of the breed. A significant (P<0.05) positive correlation was also observed between oestradiol and oestrus signs and Malabari breed exhibited prominent oestrus signs compared to Attappady Black breed.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810189447
dc.keywordsGONADOTROPINS , ATTAPPADY BLACK GOAT, MALABARI GOATS, AMH Anti-Mullerian Hormone, FSHβ Follicle Stimulating Hormone Beta,FSHR Follicle Stimulating Hormone Receptor BLAST Basic Local Alignment Search Tool BMP Bone Morphogenic Protein BMPR1B Bone Morphogenic Protein Receptor type 1B, cAMP Cyclic Adenosine Monophosphate cDNA Complementary Deoxyribonucleic Acid CL Corpus Luteum DEPC Di-ethyl Pyrocarbonate dNTP Deoxynucleotide Triphosphate EDTA Ethylene Diamine Tetra Acetic aciden_US
dc.language.isoEnglishen_US
dc.pages136p.en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHY, THRISSUR,en_US
dc.research.problemThe pattern of expression of FSHR and LHR in antral follicles of low prolific Attappady Black and high prolific Malabari goat breeds of Kerala. , Comparison of expression of FSHR and LHR in antral follicles of the two breeds. , Comparison of expression of FSHβ and LHβ in the anterior pituitary of the two breeds., Oestradiol and progesterone concentration in the serum samples.en_US
dc.subVeterinary Physiologyen_US
dc.themeGONADOTROPINS AND THEIR RECEPTORS IN ATTAPPADY BLACK AND MALABARI GOATSen_US
dc.these.typeM.V.Sc.en_US
dc.titleEXPRESSION OF GONADOTROPINS AND THEIR RECEPTORS IN ATTAPPADY BLACK AND MALABARI GOATSen_US
dc.typeThesisen_US
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