Gene Expression Analysis For Biosynthesis And Catabolism Of Glucosinolates In Rocket (Diplotaxis tenufolia L.) Under Abiotic Stress

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dc.contributor.advisorKumar, M.
dc.contributor.authorMishra, Shubhi
dc.date.accessioned2018-02-06T09:42:31Z
dc.date.available2018-02-06T09:42:31Z
dc.date.issued2017
dc.description.abstractGlucosinolates are one of the important nutritive content of rocket (Diplotaxis tenufolia L.), a leafy vegetable salad crop. They are responsible for its pepper taste, pungent aroma and medicinal properties. In this experiment, content of chlorophyll and chlorophyll fluorescence parameters were measured and biochemical analysis of glucose and glucosinolates content was done. The experiment was further followed by expression profiling of the key genes of metabolic pathway of glucosinolate using real-time PCR. The results indicated that the plant had some adaptation mechanism to mitigate the adverse effects of heat and salt stress which resist the changes in the chlorophyll content and chlorophyll fluorescence of rocket leaves as non-significant changes in the chlorophyll content and chlorophyll a fluorescence of rocket leaves.An increase in glucosinolate content was observed due to heat and salt stress in agreement with the inference derived on the basis of gene expression analysis. However in the case of roots, it appeared that glucosinolates content started decreasing after reaching the saturation point which was amply supported by higher level of expression of glucosinolates catabolism gene DtMyrosinase as observed in roots.It appeared that glucosinolates content can be increased if grown under heat and salt stress and can also be limited if grown under controlled condition. Isolation of the full length of key genes involved in glucosinolates biosynthesis namely, DtCytochromeP79F1 and catabolism namely, (DtThiol-methyl transferase) was also done through RACE. Altogether ten primers including eight primers specific to target the eight genes of glucosinolate metabolic pathway and two RACE specific primers were designed and validated during the present study which may be further utilized as tools to examine target region amplification polymorphism and expression in relation to glucosinolate biosynthesis pathway in rocket plants.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810040463
dc.keywordsRocket, Diplotaxis tenufolia, Biosynthesis, Catabolism, Glucosinolates, Biosynthesis, Abiotic stressen_US
dc.language.isoenen_US
dc.pages72+xiien_US
dc.publisherDr. Rajendra Prasad Central Agricultural University, Pusa (Samastipur)en_US
dc.subAgricultural Biotechnologyen_US
dc.subjectnullen_US
dc.themeGene Analysis For Biosynthesis and Catabolism of Glucosinolates in Rocketen_US
dc.these.typeM.Scen_US
dc.titleGene Expression Analysis For Biosynthesis And Catabolism Of Glucosinolates In Rocket (Diplotaxis tenufolia L.) Under Abiotic Stressen_US
dc.typeThesisen_US
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