FREEZABILITY AND IN VITRO FERTILITY OF MALABARI BUCK SEMEN CRYOPRESERVED IN LIPOSOME-BASED EXTENDER
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Date
2023-03-23
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COLLEGE OF VETERINARY AND ANIMAL SCIENCS, MANNUTHY, KERALA VETERINARY AND ANIMAL SCIENCES UNIVERSITY
Abstract
The present study evaluated the freezability and in vitro fertility of Malabari buck semen cryopreserved in liposome based extender. Fresh semen ejaculates collected on six different days from three adult, healthy Malabari bucks were used for the study. Initially, they were evaluated for volume of the ejaculate (mL), progressive motility (%), concentration of spermatozoa (x106/mL), viability of spermatozoa (%), morphological abnormalities of spermatozoa (%), functional membrane integrity of spermatozoa (%), acrosome integrity of spermatozoa (%), malondialdehyde concentration (nmol/mL) and cervical mucus penetration distance by spermatozoa (mm). After evaluation, fresh semen ejaculates were pooled and divided into two groups (n=6) as Group I (semen extended with tris-egg yolk based extender and Group II (semen extended with liposome based extender) and subjected to cryopreservation. Post thaw evaluation of Group I and Group II showed that progressive motility of spermatozoa (%), viability of spermatozoa (%), functional membrane integrity of spermatozoa (%), acrosome integrity of spermatozoa (%) and cervical mucus penetration distance by spermatozoa (mm) were significantly higher in Group II (p<0.05) compared to Group I. The morphological abnormalities of spermatozoa (%) and malondialdehyde concentration (nmol/mL) were significantly higher in Group I (p<0.05) compared to Group II. In vitro fertilisation rate of oocytes fertilised by semen extended with liposome based extender (Group II) was significantly higher (p<0.05) compared to those fertilised by semen extended with tris-egg yolk based extender (Group I). Therefore, it can be concluded that liposome based extender is more efficient than tris egg yolk based extender for cryopreservation of Malabari buck semen.